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Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals
BACKGROUND AND OBJECTIVES: The Escherichia coli strains are greatly important in nosocomial and community acquired infections. The aim of this study was to determine the transmission of bacterial infections using genetic analysis. MATERIALS AND METHODS: Two hundred and thirty Escherichia coli strain...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696847/ https://www.ncbi.nlm.nih.gov/pubmed/23825729 |
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author | Ramazanzadeh, Rashid Zamani, Serveh Zamani, Saman |
author_facet | Ramazanzadeh, Rashid Zamani, Serveh Zamani, Saman |
author_sort | Ramazanzadeh, Rashid |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: The Escherichia coli strains are greatly important in nosocomial and community acquired infections. The aim of this study was to determine the transmission of bacterial infections using genetic analysis. MATERIALS AND METHODS: Two hundred and thirty Escherichia coli strains, isolated from different clinical samples, were characterized by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique. The results and the similarity between the strains were determined on the basis of Jaccard similarity coefficient in the SAHN program of the NTSYS-pc software. RESULTS: The ERIC–PCR profiles allowed typing of the 230 isolates into 205 ERIC-types which were grouped into twenty main clusters (C01–C20).The first group makes up 4.34% of the total isolates. Out of the 230 isolates, 34.2% belonged to D phylogenic group which were associated with extra-intestinal samples. CONCLUSION: Our results showed high diversity in E. coli isolates indicating low rate of hospital infection in our university hospitals. The majority of the isolates belonged to the D phylogenic group. |
format | Online Article Text |
id | pubmed-3696847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-36968472013-07-02 Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals Ramazanzadeh, Rashid Zamani, Serveh Zamani, Saman Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: The Escherichia coli strains are greatly important in nosocomial and community acquired infections. The aim of this study was to determine the transmission of bacterial infections using genetic analysis. MATERIALS AND METHODS: Two hundred and thirty Escherichia coli strains, isolated from different clinical samples, were characterized by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique. The results and the similarity between the strains were determined on the basis of Jaccard similarity coefficient in the SAHN program of the NTSYS-pc software. RESULTS: The ERIC–PCR profiles allowed typing of the 230 isolates into 205 ERIC-types which were grouped into twenty main clusters (C01–C20).The first group makes up 4.34% of the total isolates. Out of the 230 isolates, 34.2% belonged to D phylogenic group which were associated with extra-intestinal samples. CONCLUSION: Our results showed high diversity in E. coli isolates indicating low rate of hospital infection in our university hospitals. The majority of the isolates belonged to the D phylogenic group. Tehran University of Medical Sciences 2013-06 /pmc/articles/PMC3696847/ /pubmed/23825729 Text en © 2013 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ramazanzadeh, Rashid Zamani, Serveh Zamani, Saman Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals |
title | Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals |
title_full | Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals |
title_fullStr | Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals |
title_full_unstemmed | Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals |
title_short | Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)–PCR technique in Sanandaj hospitals |
title_sort | genetic diversity in clinical isolates of escherichia coli by enterobacterial repetitive intergenic consensus (eric)–pcr technique in sanandaj hospitals |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696847/ https://www.ncbi.nlm.nih.gov/pubmed/23825729 |
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