Cargando…
Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments
Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n = 87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697410/ https://www.ncbi.nlm.nih.gov/pubmed/23844278 http://dx.doi.org/10.1155/2013/657016 |
_version_ | 1782275195324596224 |
---|---|
author | O'Daly, José A. Spinetti, Humberto M. Gleason, Joe Rodríguez, María B. |
author_facet | O'Daly, José A. Spinetti, Humberto M. Gleason, Joe Rodríguez, María B. |
author_sort | O'Daly, José A. |
collection | PubMed |
description | Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n = 87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from January 1994 to August 2000. Time for remission of lesions were spontaneous remission (SR) 7 weeks; Glucantime (Glu) chemotherapy 9 weeks; immunotherapy with La, Lv, Lb, and Lch amastigotes Tosyl-Lysil Chloromethyl-ketone (TLCK) treated and Nonidet P-40(NP-40) extracted (VT) 7 weeks. Delayed type hypersensitivity (DTH) response with leishmanine intradermic reaction (IDR) was higher in CL patients than healthy controls (P < 0.05) and increased in active secondary versus primary infection (P < 0.001) with diagnostic value 1.74 for active infection and 1.81 postclinical remission. Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P < 0.001), with a diagnostic value from 1.50 to 1.84. Immunoblottings antigenic bands frequency as well as Integral Optical Density (IOD) Area Densitometry decreased with sera from SR, after Glu or VT treatments in CL volunteers. Intracellular parasitism is due to normal antibodies recognizing parasite antigens after inoculation by vector. VT vaccine induced mainly cellular immunity, for remission of lesions and protection from CL infection. |
format | Online Article Text |
id | pubmed-3697410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36974102013-07-10 Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments O'Daly, José A. Spinetti, Humberto M. Gleason, Joe Rodríguez, María B. J Parasitol Res Clinical Study Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n = 87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from January 1994 to August 2000. Time for remission of lesions were spontaneous remission (SR) 7 weeks; Glucantime (Glu) chemotherapy 9 weeks; immunotherapy with La, Lv, Lb, and Lch amastigotes Tosyl-Lysil Chloromethyl-ketone (TLCK) treated and Nonidet P-40(NP-40) extracted (VT) 7 weeks. Delayed type hypersensitivity (DTH) response with leishmanine intradermic reaction (IDR) was higher in CL patients than healthy controls (P < 0.05) and increased in active secondary versus primary infection (P < 0.001) with diagnostic value 1.74 for active infection and 1.81 postclinical remission. Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P < 0.001), with a diagnostic value from 1.50 to 1.84. Immunoblottings antigenic bands frequency as well as Integral Optical Density (IOD) Area Densitometry decreased with sera from SR, after Glu or VT treatments in CL volunteers. Intracellular parasitism is due to normal antibodies recognizing parasite antigens after inoculation by vector. VT vaccine induced mainly cellular immunity, for remission of lesions and protection from CL infection. Hindawi Publishing Corporation 2013 2013-06-13 /pmc/articles/PMC3697410/ /pubmed/23844278 http://dx.doi.org/10.1155/2013/657016 Text en Copyright © 2013 José A. O'Daly et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Study O'Daly, José A. Spinetti, Humberto M. Gleason, Joe Rodríguez, María B. Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
title | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
title_full | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
title_fullStr | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
title_full_unstemmed | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
title_short | Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments |
title_sort | clinical and immunological analysis of cutaneous leishmaniasis before and after different treatments |
topic | Clinical Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697410/ https://www.ncbi.nlm.nih.gov/pubmed/23844278 http://dx.doi.org/10.1155/2013/657016 |
work_keys_str_mv | AT odalyjosea clinicalandimmunologicalanalysisofcutaneousleishmaniasisbeforeandafterdifferenttreatments AT spinettihumbertom clinicalandimmunologicalanalysisofcutaneousleishmaniasisbeforeandafterdifferenttreatments AT gleasonjoe clinicalandimmunologicalanalysisofcutaneousleishmaniasisbeforeandafterdifferenttreatments AT rodriguezmariab clinicalandimmunologicalanalysisofcutaneousleishmaniasisbeforeandafterdifferenttreatments |