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Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments

Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n = 87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from...

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Autores principales: O'Daly, José A., Spinetti, Humberto M., Gleason, Joe, Rodríguez, María B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697410/
https://www.ncbi.nlm.nih.gov/pubmed/23844278
http://dx.doi.org/10.1155/2013/657016
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author O'Daly, José A.
Spinetti, Humberto M.
Gleason, Joe
Rodríguez, María B.
author_facet O'Daly, José A.
Spinetti, Humberto M.
Gleason, Joe
Rodríguez, María B.
author_sort O'Daly, José A.
collection PubMed
description Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n = 87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from January 1994 to August 2000. Time for remission of lesions were spontaneous remission (SR) 7 weeks; Glucantime (Glu) chemotherapy 9 weeks; immunotherapy with La, Lv, Lb, and Lch amastigotes Tosyl-Lysil Chloromethyl-ketone (TLCK) treated and Nonidet P-40(NP-40) extracted (VT) 7 weeks. Delayed type hypersensitivity (DTH) response with leishmanine intradermic reaction (IDR) was higher in CL patients than healthy controls (P < 0.05) and increased in active secondary versus primary infection (P < 0.001) with diagnostic value 1.74 for active infection and 1.81 postclinical remission. Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P < 0.001), with a diagnostic value from 1.50 to 1.84. Immunoblottings antigenic bands frequency as well as Integral Optical Density (IOD) Area Densitometry decreased with sera from SR, after Glu or VT treatments in CL volunteers. Intracellular parasitism is due to normal antibodies recognizing parasite antigens after inoculation by vector. VT vaccine induced mainly cellular immunity, for remission of lesions and protection from CL infection.
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spelling pubmed-36974102013-07-10 Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments O'Daly, José A. Spinetti, Humberto M. Gleason, Joe Rodríguez, María B. J Parasitol Res Clinical Study Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n = 87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from January 1994 to August 2000. Time for remission of lesions were spontaneous remission (SR) 7 weeks; Glucantime (Glu) chemotherapy 9 weeks; immunotherapy with La, Lv, Lb, and Lch amastigotes Tosyl-Lysil Chloromethyl-ketone (TLCK) treated and Nonidet P-40(NP-40) extracted (VT) 7 weeks. Delayed type hypersensitivity (DTH) response with leishmanine intradermic reaction (IDR) was higher in CL patients than healthy controls (P < 0.05) and increased in active secondary versus primary infection (P < 0.001) with diagnostic value 1.74 for active infection and 1.81 postclinical remission. Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P < 0.001), with a diagnostic value from 1.50 to 1.84. Immunoblottings antigenic bands frequency as well as Integral Optical Density (IOD) Area Densitometry decreased with sera from SR, after Glu or VT treatments in CL volunteers. Intracellular parasitism is due to normal antibodies recognizing parasite antigens after inoculation by vector. VT vaccine induced mainly cellular immunity, for remission of lesions and protection from CL infection. Hindawi Publishing Corporation 2013 2013-06-13 /pmc/articles/PMC3697410/ /pubmed/23844278 http://dx.doi.org/10.1155/2013/657016 Text en Copyright © 2013 José A. O'Daly et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
O'Daly, José A.
Spinetti, Humberto M.
Gleason, Joe
Rodríguez, María B.
Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments
title Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments
title_full Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments
title_fullStr Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments
title_full_unstemmed Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments
title_short Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments
title_sort clinical and immunological analysis of cutaneous leishmaniasis before and after different treatments
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697410/
https://www.ncbi.nlm.nih.gov/pubmed/23844278
http://dx.doi.org/10.1155/2013/657016
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