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A comparison of sex-specific immune signatures in Gulf War illness and chronic fatigue syndrome

BACKGROUND: Though potentially linked to the basic physiology of stress response we still have no clear understanding of Gulf War Illness (GWI), a debilitating condition presenting complex immune, endocrine and neurological symptoms. Here we compared male (n = 20) and female (n = 10) veterans with G...

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Autores principales: Smylie, Anne Liese, Broderick, Gordon, Fernandes, Henrique, Razdan, Shirin, Barnes, Zachary, Collado, Fanny, Sol, Connie, Fletcher, Mary Ann, Klimas, Nancy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698072/
https://www.ncbi.nlm.nih.gov/pubmed/23800166
http://dx.doi.org/10.1186/1471-2172-14-29
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author Smylie, Anne Liese
Broderick, Gordon
Fernandes, Henrique
Razdan, Shirin
Barnes, Zachary
Collado, Fanny
Sol, Connie
Fletcher, Mary Ann
Klimas, Nancy
author_facet Smylie, Anne Liese
Broderick, Gordon
Fernandes, Henrique
Razdan, Shirin
Barnes, Zachary
Collado, Fanny
Sol, Connie
Fletcher, Mary Ann
Klimas, Nancy
author_sort Smylie, Anne Liese
collection PubMed
description BACKGROUND: Though potentially linked to the basic physiology of stress response we still have no clear understanding of Gulf War Illness (GWI), a debilitating condition presenting complex immune, endocrine and neurological symptoms. Here we compared male (n = 20) and female (n = 10) veterans with GWI separately against their healthy counterparts (n = 21 male, n = 9 female) as well as subjects with chronic fatigue syndrome/ myalgic encephalomyelitis (CFS/ME) (n = 12 male, n = 10 female). METHODS: Subjects were assessed using a Graded eXercise Test (GXT) with blood drawn prior to exercise, at peak effort (VO2 max) and 4-hours post exercise. Using chemiluminescent imaging we measured the concentrations of IL-1a, 1b, 2, 4, 5, 6, 8, 10, 12 (p70), 13, 15, 17 and 23, IFNγ, TNFα and TNFβ in plasma samples from each phase of exercise. Linear classification models were constructed using stepwise variable selection to identify cytokine co-expression patterns characteristic of each subject group. RESULTS: Classification accuracies in excess of 80% were obtained using between 2 and 5 cytokine markers. Common to both GWI and CFS, IL-10 and IL-23 expression contributed in an illness and time-dependent manner, accompanied in male subjects by NK and Th1 markers IL-12, IL-15, IL-2 and IFNγ. In female GWI and CFS subjects IL-10 was again identified as a delineator but this time in the context of IL-17 and Th2 markers IL-4 and IL-5. Exercise response also differed between sexes: male GWI subjects presented characteristic cytokine signatures at rest but not at peak effort whereas the opposite was true for female subjects. CONCLUSIONS: Though individual markers varied, results collectively supported involvement of the IL-23/Th17/IL-17 axis in the delineation of GWI and CFS in a sex-specific way.
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spelling pubmed-36980722013-07-02 A comparison of sex-specific immune signatures in Gulf War illness and chronic fatigue syndrome Smylie, Anne Liese Broderick, Gordon Fernandes, Henrique Razdan, Shirin Barnes, Zachary Collado, Fanny Sol, Connie Fletcher, Mary Ann Klimas, Nancy BMC Immunol Research Article BACKGROUND: Though potentially linked to the basic physiology of stress response we still have no clear understanding of Gulf War Illness (GWI), a debilitating condition presenting complex immune, endocrine and neurological symptoms. Here we compared male (n = 20) and female (n = 10) veterans with GWI separately against their healthy counterparts (n = 21 male, n = 9 female) as well as subjects with chronic fatigue syndrome/ myalgic encephalomyelitis (CFS/ME) (n = 12 male, n = 10 female). METHODS: Subjects were assessed using a Graded eXercise Test (GXT) with blood drawn prior to exercise, at peak effort (VO2 max) and 4-hours post exercise. Using chemiluminescent imaging we measured the concentrations of IL-1a, 1b, 2, 4, 5, 6, 8, 10, 12 (p70), 13, 15, 17 and 23, IFNγ, TNFα and TNFβ in plasma samples from each phase of exercise. Linear classification models were constructed using stepwise variable selection to identify cytokine co-expression patterns characteristic of each subject group. RESULTS: Classification accuracies in excess of 80% were obtained using between 2 and 5 cytokine markers. Common to both GWI and CFS, IL-10 and IL-23 expression contributed in an illness and time-dependent manner, accompanied in male subjects by NK and Th1 markers IL-12, IL-15, IL-2 and IFNγ. In female GWI and CFS subjects IL-10 was again identified as a delineator but this time in the context of IL-17 and Th2 markers IL-4 and IL-5. Exercise response also differed between sexes: male GWI subjects presented characteristic cytokine signatures at rest but not at peak effort whereas the opposite was true for female subjects. CONCLUSIONS: Though individual markers varied, results collectively supported involvement of the IL-23/Th17/IL-17 axis in the delineation of GWI and CFS in a sex-specific way. BioMed Central 2013-06-25 /pmc/articles/PMC3698072/ /pubmed/23800166 http://dx.doi.org/10.1186/1471-2172-14-29 Text en Copyright © 2013 Smylie et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Smylie, Anne Liese
Broderick, Gordon
Fernandes, Henrique
Razdan, Shirin
Barnes, Zachary
Collado, Fanny
Sol, Connie
Fletcher, Mary Ann
Klimas, Nancy
A comparison of sex-specific immune signatures in Gulf War illness and chronic fatigue syndrome
title A comparison of sex-specific immune signatures in Gulf War illness and chronic fatigue syndrome
title_full A comparison of sex-specific immune signatures in Gulf War illness and chronic fatigue syndrome
title_fullStr A comparison of sex-specific immune signatures in Gulf War illness and chronic fatigue syndrome
title_full_unstemmed A comparison of sex-specific immune signatures in Gulf War illness and chronic fatigue syndrome
title_short A comparison of sex-specific immune signatures in Gulf War illness and chronic fatigue syndrome
title_sort comparison of sex-specific immune signatures in gulf war illness and chronic fatigue syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698072/
https://www.ncbi.nlm.nih.gov/pubmed/23800166
http://dx.doi.org/10.1186/1471-2172-14-29
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