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Multicolor lineage tracing methods and intestinal tumors
The generation of chimeras, which is now a standard technology for producing gene modified mutant mice, was originally developed as a tool for developmental biology. However, the application of conventional single marker chimeric mice for developmental study was initially limited. This situation has...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Japan
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698421/ https://www.ncbi.nlm.nih.gov/pubmed/23307044 http://dx.doi.org/10.1007/s00535-012-0736-3 |
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author | Yanai, Hirotsugu Tanaka, Toshihiro Ueno, Hiroo |
author_facet | Yanai, Hirotsugu Tanaka, Toshihiro Ueno, Hiroo |
author_sort | Yanai, Hirotsugu |
collection | PubMed |
description | The generation of chimeras, which is now a standard technology for producing gene modified mutant mice, was originally developed as a tool for developmental biology. However, the application of conventional single marker chimeric mice for developmental study was initially limited. This situation has been dramatically changed by development of multicolor chimeric mice using various kinds of fluorescent proteins. Now using our technology, up to ten different clones could be distinguished by their colors, which enable us to perform more accurate statistical analyses and lineage tracing experiments than by conventional methods. This method could be applied to visualize not only cell turnover of normal stem cells but also cancer development of live tissues in vivo. In the present review, we will discuss how these methods have been developed and what questions they are now answering by mainly focusing on intestinal stem cells and intestinal tumors. |
format | Online Article Text |
id | pubmed-3698421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-36984212013-07-10 Multicolor lineage tracing methods and intestinal tumors Yanai, Hirotsugu Tanaka, Toshihiro Ueno, Hiroo J Gastroenterol Review The generation of chimeras, which is now a standard technology for producing gene modified mutant mice, was originally developed as a tool for developmental biology. However, the application of conventional single marker chimeric mice for developmental study was initially limited. This situation has been dramatically changed by development of multicolor chimeric mice using various kinds of fluorescent proteins. Now using our technology, up to ten different clones could be distinguished by their colors, which enable us to perform more accurate statistical analyses and lineage tracing experiments than by conventional methods. This method could be applied to visualize not only cell turnover of normal stem cells but also cancer development of live tissues in vivo. In the present review, we will discuss how these methods have been developed and what questions they are now answering by mainly focusing on intestinal stem cells and intestinal tumors. Springer Japan 2013-01-11 2013-04 /pmc/articles/PMC3698421/ /pubmed/23307044 http://dx.doi.org/10.1007/s00535-012-0736-3 Text en © Springer Japan 2013 |
spellingShingle | Review Yanai, Hirotsugu Tanaka, Toshihiro Ueno, Hiroo Multicolor lineage tracing methods and intestinal tumors |
title | Multicolor lineage tracing methods and intestinal tumors |
title_full | Multicolor lineage tracing methods and intestinal tumors |
title_fullStr | Multicolor lineage tracing methods and intestinal tumors |
title_full_unstemmed | Multicolor lineage tracing methods and intestinal tumors |
title_short | Multicolor lineage tracing methods and intestinal tumors |
title_sort | multicolor lineage tracing methods and intestinal tumors |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698421/ https://www.ncbi.nlm.nih.gov/pubmed/23307044 http://dx.doi.org/10.1007/s00535-012-0736-3 |
work_keys_str_mv | AT yanaihirotsugu multicolorlineagetracingmethodsandintestinaltumors AT tanakatoshihiro multicolorlineagetracingmethodsandintestinaltumors AT uenohiroo multicolorlineagetracingmethodsandintestinaltumors |