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Reduced Neointima Formation After Arterial Injury in CD4−/− Mice Is Mediated by CD8(+)CD28(hi) T Cells
BACKGROUND: CD8(+) T‐cell activation, characterized by increased CD28 expression, reduces neointima formation after arterial injury in mice. The CD8(+)CD28(hi) phenotype is associated with increased effector function. In this study, we used a mouse model that has CD8(+) but no CD4(+) T cells (CD4−/−...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698777/ https://www.ncbi.nlm.nih.gov/pubmed/23702879 http://dx.doi.org/10.1161/JAHA.113.000155 |
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author | Dimayuga, Paul C. Chyu, Kuang‐Yuh Lio, Wai Man Zhao, Xiaoning Yano, Juliana Zhou, Jianchang Honjo, Tomoyuki Shah, Prediman K. Cercek, Bojan |
author_facet | Dimayuga, Paul C. Chyu, Kuang‐Yuh Lio, Wai Man Zhao, Xiaoning Yano, Juliana Zhou, Jianchang Honjo, Tomoyuki Shah, Prediman K. Cercek, Bojan |
author_sort | Dimayuga, Paul C. |
collection | PubMed |
description | BACKGROUND: CD8(+) T‐cell activation, characterized by increased CD28 expression, reduces neointima formation after arterial injury in mice. The CD8(+)CD28(hi) phenotype is associated with increased effector function. In this study, we used a mouse model that has CD8(+) but no CD4(+) T cells (CD4−/−) to assess the role of CD8(+) T cells and test the function of CD8(+)CD28(hi) T cells in modulating neointima formation after arterial injury. METHODS AND RESULTS: Neointima formation after pericarotid arterial cuff injury was significantly less in CD4−/− mice compared with wild‐type (WT) mice. Negligible baseline lytic activity by splenic CD8(+) T cells from uninjured WT mice against target syngeneic smooth muscle cells was significantly increased 7 days after injury. Interestingly, CD8(+) T cells from uninjured CD4−/− mice had significant lytic activity at baseline that remained elevated 7 days after injury. CD8(+) T‐cell lytic activity was significantly reduced by depletion of CD28(hi) cells. CD8(+)CD28(hi) T cells adoptively transferred into recipient Rag‐1−/− mice significantly reduced neointima formation compared with CD8(+)CD28(+) T‐cell recipient mice. CONCLUSIONS: CD8(+) T cells reduced neointima formation after arterial injury, attributed in part to increased function of the CD8(+)CD28(hi) phenotype. |
format | Online Article Text |
id | pubmed-3698777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-36987772013-09-03 Reduced Neointima Formation After Arterial Injury in CD4−/− Mice Is Mediated by CD8(+)CD28(hi) T Cells Dimayuga, Paul C. Chyu, Kuang‐Yuh Lio, Wai Man Zhao, Xiaoning Yano, Juliana Zhou, Jianchang Honjo, Tomoyuki Shah, Prediman K. Cercek, Bojan J Am Heart Assoc Original Research BACKGROUND: CD8(+) T‐cell activation, characterized by increased CD28 expression, reduces neointima formation after arterial injury in mice. The CD8(+)CD28(hi) phenotype is associated with increased effector function. In this study, we used a mouse model that has CD8(+) but no CD4(+) T cells (CD4−/−) to assess the role of CD8(+) T cells and test the function of CD8(+)CD28(hi) T cells in modulating neointima formation after arterial injury. METHODS AND RESULTS: Neointima formation after pericarotid arterial cuff injury was significantly less in CD4−/− mice compared with wild‐type (WT) mice. Negligible baseline lytic activity by splenic CD8(+) T cells from uninjured WT mice against target syngeneic smooth muscle cells was significantly increased 7 days after injury. Interestingly, CD8(+) T cells from uninjured CD4−/− mice had significant lytic activity at baseline that remained elevated 7 days after injury. CD8(+) T‐cell lytic activity was significantly reduced by depletion of CD28(hi) cells. CD8(+)CD28(hi) T cells adoptively transferred into recipient Rag‐1−/− mice significantly reduced neointima formation compared with CD8(+)CD28(+) T‐cell recipient mice. CONCLUSIONS: CD8(+) T cells reduced neointima formation after arterial injury, attributed in part to increased function of the CD8(+)CD28(hi) phenotype. Blackwell Publishing Ltd 2013-06-21 /pmc/articles/PMC3698777/ /pubmed/23702879 http://dx.doi.org/10.1161/JAHA.113.000155 Text en © 2013 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley-Blackwell. http://creativecommons.org/licenses/by/2.5/ This is an Open Access article under the terms of the Creative Commons Attribution Noncommercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Research Dimayuga, Paul C. Chyu, Kuang‐Yuh Lio, Wai Man Zhao, Xiaoning Yano, Juliana Zhou, Jianchang Honjo, Tomoyuki Shah, Prediman K. Cercek, Bojan Reduced Neointima Formation After Arterial Injury in CD4−/− Mice Is Mediated by CD8(+)CD28(hi) T Cells |
title | Reduced Neointima Formation After Arterial Injury in CD4−/− Mice Is Mediated by CD8(+)CD28(hi) T Cells |
title_full | Reduced Neointima Formation After Arterial Injury in CD4−/− Mice Is Mediated by CD8(+)CD28(hi) T Cells |
title_fullStr | Reduced Neointima Formation After Arterial Injury in CD4−/− Mice Is Mediated by CD8(+)CD28(hi) T Cells |
title_full_unstemmed | Reduced Neointima Formation After Arterial Injury in CD4−/− Mice Is Mediated by CD8(+)CD28(hi) T Cells |
title_short | Reduced Neointima Formation After Arterial Injury in CD4−/− Mice Is Mediated by CD8(+)CD28(hi) T Cells |
title_sort | reduced neointima formation after arterial injury in cd4−/− mice is mediated by cd8(+)cd28(hi) t cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698777/ https://www.ncbi.nlm.nih.gov/pubmed/23702879 http://dx.doi.org/10.1161/JAHA.113.000155 |
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