Cargando…
hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding
This work builds upon our findings that proteins secreted by hESCs exhibit pro-regenerative activity, and determines that hESC-conditioned medium robustly enhances the proliferation of both muscle and neural progenitor cells. Importantly, this work establishes that it is the proteins that bind hepar...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701111/ https://www.ncbi.nlm.nih.gov/pubmed/23793469 |
_version_ | 1782275596530745344 |
---|---|
author | Yousef, Hanadie Conboy, Michael J. Li, Ju Zeiderman, Matthew Vazin, Tandis Schlesinger, Christina Schaffer, David V. Conboy, Irina M. |
author_facet | Yousef, Hanadie Conboy, Michael J. Li, Ju Zeiderman, Matthew Vazin, Tandis Schlesinger, Christina Schaffer, David V. Conboy, Irina M. |
author_sort | Yousef, Hanadie |
collection | PubMed |
description | This work builds upon our findings that proteins secreted by hESCs exhibit pro-regenerative activity, and determines that hESC-conditioned medium robustly enhances the proliferation of both muscle and neural progenitor cells. Importantly, this work establishes that it is the proteins that bind heparin which are responsible for the pro-myogenic effects of hESC-conditioned medium, and indicates that this strategy is suitable for enriching the potentially therapeutic factors. Additionally, this work shows that hESC-secreted proteins act independently of the mitogen FGF-2, and suggests that FGF-2 is unlikely to be a pro-aging molecule in the physiological decline of old muscle repair. Moreover, hESC-secreted factors improve the viability of human cortical neurons in an Alzheimer's disease (AD) model, suggesting that these factors can enhance the maintenance and regeneration of multiple tissues in the aging body. |
format | Online Article Text |
id | pubmed-3701111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-37011112013-07-11 hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding Yousef, Hanadie Conboy, Michael J. Li, Ju Zeiderman, Matthew Vazin, Tandis Schlesinger, Christina Schaffer, David V. Conboy, Irina M. Aging (Albany NY) Research Paper This work builds upon our findings that proteins secreted by hESCs exhibit pro-regenerative activity, and determines that hESC-conditioned medium robustly enhances the proliferation of both muscle and neural progenitor cells. Importantly, this work establishes that it is the proteins that bind heparin which are responsible for the pro-myogenic effects of hESC-conditioned medium, and indicates that this strategy is suitable for enriching the potentially therapeutic factors. Additionally, this work shows that hESC-secreted proteins act independently of the mitogen FGF-2, and suggests that FGF-2 is unlikely to be a pro-aging molecule in the physiological decline of old muscle repair. Moreover, hESC-secreted factors improve the viability of human cortical neurons in an Alzheimer's disease (AD) model, suggesting that these factors can enhance the maintenance and regeneration of multiple tissues in the aging body. Impact Journals LLC 2013-05-23 /pmc/articles/PMC3701111/ /pubmed/23793469 Text en Copyright: © 2013 Yousef et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
spellingShingle | Research Paper Yousef, Hanadie Conboy, Michael J. Li, Ju Zeiderman, Matthew Vazin, Tandis Schlesinger, Christina Schaffer, David V. Conboy, Irina M. hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding |
title | hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding |
title_full | hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding |
title_fullStr | hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding |
title_full_unstemmed | hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding |
title_short | hESC-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding |
title_sort | hesc-secreted proteins can be enriched for multiple regenerative therapies by heparin-binding |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701111/ https://www.ncbi.nlm.nih.gov/pubmed/23793469 |
work_keys_str_mv | AT yousefhanadie hescsecretedproteinscanbeenrichedformultipleregenerativetherapiesbyheparinbinding AT conboymichaelj hescsecretedproteinscanbeenrichedformultipleregenerativetherapiesbyheparinbinding AT liju hescsecretedproteinscanbeenrichedformultipleregenerativetherapiesbyheparinbinding AT zeidermanmatthew hescsecretedproteinscanbeenrichedformultipleregenerativetherapiesbyheparinbinding AT vazintandis hescsecretedproteinscanbeenrichedformultipleregenerativetherapiesbyheparinbinding AT schlesingerchristina hescsecretedproteinscanbeenrichedformultipleregenerativetherapiesbyheparinbinding AT schafferdavidv hescsecretedproteinscanbeenrichedformultipleregenerativetherapiesbyheparinbinding AT conboyirinam hescsecretedproteinscanbeenrichedformultipleregenerativetherapiesbyheparinbinding |