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Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney
The effects of cadmium and copper on activity of common carp head kidney hematopoietic tissue were evaluated. The fish were subjected to short-term (3 h, Cd-s and Cu-s) or long-term (4 weeks, Cd-l and Cu-l) exposures to 100 % 96hLC(50) or 10 % 96hLC(50), respectively. Head kidneys were isolated week...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701137/ https://www.ncbi.nlm.nih.gov/pubmed/23086609 http://dx.doi.org/10.1007/s10695-012-9738-6 |
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author | Kondera, Elzbieta Witeska, Malgorzata |
author_facet | Kondera, Elzbieta Witeska, Malgorzata |
author_sort | Kondera, Elzbieta |
collection | PubMed |
description | The effects of cadmium and copper on activity of common carp head kidney hematopoietic tissue were evaluated. The fish were subjected to short-term (3 h, Cd-s and Cu-s) or long-term (4 weeks, Cd-l and Cu-l) exposures to 100 % 96hLC(50) or 10 % 96hLC(50), respectively. Head kidneys were isolated weekly from 5 fish of each group for 4 weeks (post-short-term exposure and during long-term exposure). Percentage of early blast cells among the hematopoietic precursors was calculated. Proliferative and apoptotic activity were evaluated using immunocytochemical staining for proliferating cell nuclear antigen (PCNA) and caspase 3, respectively. Hematopoietic activity was calculated as the ratio of proliferating to apoptotic cells. All metal exposures induced an increase in frequency of early blast cells. The frequency of proliferating (PCNA-positive) cells also significantly increased. A considerable and significant increase in the frequency of apoptotic cells was the most pronounced effect of metal exposures. Both short-term and long-term treatments caused similar effects, but in case of Cd exposures, the reaction was more pronounced. All metal exposures reduced hematopoietic potential of fish measured as the ratio of proliferating to apoptotic precursor cell frequency. However, in all cases, hematopoietic activity was higher than 1 showing that the rate of repair of hematopoietic tissue prevailed over destruction. |
format | Online Article Text |
id | pubmed-3701137 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-37011372013-07-12 Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney Kondera, Elzbieta Witeska, Malgorzata Fish Physiol Biochem Article The effects of cadmium and copper on activity of common carp head kidney hematopoietic tissue were evaluated. The fish were subjected to short-term (3 h, Cd-s and Cu-s) or long-term (4 weeks, Cd-l and Cu-l) exposures to 100 % 96hLC(50) or 10 % 96hLC(50), respectively. Head kidneys were isolated weekly from 5 fish of each group for 4 weeks (post-short-term exposure and during long-term exposure). Percentage of early blast cells among the hematopoietic precursors was calculated. Proliferative and apoptotic activity were evaluated using immunocytochemical staining for proliferating cell nuclear antigen (PCNA) and caspase 3, respectively. Hematopoietic activity was calculated as the ratio of proliferating to apoptotic cells. All metal exposures induced an increase in frequency of early blast cells. The frequency of proliferating (PCNA-positive) cells also significantly increased. A considerable and significant increase in the frequency of apoptotic cells was the most pronounced effect of metal exposures. Both short-term and long-term treatments caused similar effects, but in case of Cd exposures, the reaction was more pronounced. All metal exposures reduced hematopoietic potential of fish measured as the ratio of proliferating to apoptotic precursor cell frequency. However, in all cases, hematopoietic activity was higher than 1 showing that the rate of repair of hematopoietic tissue prevailed over destruction. Springer Netherlands 2012-10-20 2013 /pmc/articles/PMC3701137/ /pubmed/23086609 http://dx.doi.org/10.1007/s10695-012-9738-6 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Article Kondera, Elzbieta Witeska, Malgorzata Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney |
title | Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney |
title_full | Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney |
title_fullStr | Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney |
title_full_unstemmed | Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney |
title_short | Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney |
title_sort | cadmium and copper reduce hematopoietic potential in common carp (cyprinus carpio l.) head kidney |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701137/ https://www.ncbi.nlm.nih.gov/pubmed/23086609 http://dx.doi.org/10.1007/s10695-012-9738-6 |
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