Cargando…

Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids

Mannitol (Mtl) fermentation, with the subsequent production of acid, is a species signature of Staphylococcus aureus, and discriminates it from most other members of the genus. Inactivation of the gene mtlD, encoding Mtl-1-P dehydrogenase was found to markedly reduce survival in the presence of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Kenny, John G., Moran, Josephine, Kolar, Stacey L., Ulanov, Alexander, Li, Zhong, Shaw, Lindsey N., Josefsson, Elisabet, Horsburgh, Malcolm J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701532/
https://www.ncbi.nlm.nih.gov/pubmed/23861785
http://dx.doi.org/10.1371/journal.pone.0067698
_version_ 1782275658733322240
author Kenny, John G.
Moran, Josephine
Kolar, Stacey L.
Ulanov, Alexander
Li, Zhong
Shaw, Lindsey N.
Josefsson, Elisabet
Horsburgh, Malcolm J.
author_facet Kenny, John G.
Moran, Josephine
Kolar, Stacey L.
Ulanov, Alexander
Li, Zhong
Shaw, Lindsey N.
Josefsson, Elisabet
Horsburgh, Malcolm J.
author_sort Kenny, John G.
collection PubMed
description Mannitol (Mtl) fermentation, with the subsequent production of acid, is a species signature of Staphylococcus aureus, and discriminates it from most other members of the genus. Inactivation of the gene mtlD, encoding Mtl-1-P dehydrogenase was found to markedly reduce survival in the presence of the antimicrobial fatty acid, linoleic acid. We demonstrate that the sugar alcohol has a potentiating action for this membrane-acting antimicrobial. Analysis of cellular metabolites revealed that, during exponential growth, the mtlD mutant accumulated high levels of Mtl and Mtl-P. The latter metabolite was not detected in its isogenic parent strain or a deletion mutant of the entire mtlABFD operon. In addition, the mtlD mutant strain exhibited a decreased MIC for H(2)O(2), however virulence was unaffected in a model of septic arthritis.
format Online
Article
Text
id pubmed-3701532
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-37015322013-07-16 Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids Kenny, John G. Moran, Josephine Kolar, Stacey L. Ulanov, Alexander Li, Zhong Shaw, Lindsey N. Josefsson, Elisabet Horsburgh, Malcolm J. PLoS One Research Article Mannitol (Mtl) fermentation, with the subsequent production of acid, is a species signature of Staphylococcus aureus, and discriminates it from most other members of the genus. Inactivation of the gene mtlD, encoding Mtl-1-P dehydrogenase was found to markedly reduce survival in the presence of the antimicrobial fatty acid, linoleic acid. We demonstrate that the sugar alcohol has a potentiating action for this membrane-acting antimicrobial. Analysis of cellular metabolites revealed that, during exponential growth, the mtlD mutant accumulated high levels of Mtl and Mtl-P. The latter metabolite was not detected in its isogenic parent strain or a deletion mutant of the entire mtlABFD operon. In addition, the mtlD mutant strain exhibited a decreased MIC for H(2)O(2), however virulence was unaffected in a model of septic arthritis. Public Library of Science 2013-07-04 /pmc/articles/PMC3701532/ /pubmed/23861785 http://dx.doi.org/10.1371/journal.pone.0067698 Text en © 2013 Kenny et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kenny, John G.
Moran, Josephine
Kolar, Stacey L.
Ulanov, Alexander
Li, Zhong
Shaw, Lindsey N.
Josefsson, Elisabet
Horsburgh, Malcolm J.
Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids
title Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids
title_full Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids
title_fullStr Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids
title_full_unstemmed Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids
title_short Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids
title_sort mannitol utilisation is required for protection of staphylococcus aureus from human skin antimicrobial fatty acids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701532/
https://www.ncbi.nlm.nih.gov/pubmed/23861785
http://dx.doi.org/10.1371/journal.pone.0067698
work_keys_str_mv AT kennyjohng mannitolutilisationisrequiredforprotectionofstaphylococcusaureusfromhumanskinantimicrobialfattyacids
AT moranjosephine mannitolutilisationisrequiredforprotectionofstaphylococcusaureusfromhumanskinantimicrobialfattyacids
AT kolarstaceyl mannitolutilisationisrequiredforprotectionofstaphylococcusaureusfromhumanskinantimicrobialfattyacids
AT ulanovalexander mannitolutilisationisrequiredforprotectionofstaphylococcusaureusfromhumanskinantimicrobialfattyacids
AT lizhong mannitolutilisationisrequiredforprotectionofstaphylococcusaureusfromhumanskinantimicrobialfattyacids
AT shawlindseyn mannitolutilisationisrequiredforprotectionofstaphylococcusaureusfromhumanskinantimicrobialfattyacids
AT josefssonelisabet mannitolutilisationisrequiredforprotectionofstaphylococcusaureusfromhumanskinantimicrobialfattyacids
AT horsburghmalcolmj mannitolutilisationisrequiredforprotectionofstaphylococcusaureusfromhumanskinantimicrobialfattyacids