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Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids
Mannitol (Mtl) fermentation, with the subsequent production of acid, is a species signature of Staphylococcus aureus, and discriminates it from most other members of the genus. Inactivation of the gene mtlD, encoding Mtl-1-P dehydrogenase was found to markedly reduce survival in the presence of the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701532/ https://www.ncbi.nlm.nih.gov/pubmed/23861785 http://dx.doi.org/10.1371/journal.pone.0067698 |
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author | Kenny, John G. Moran, Josephine Kolar, Stacey L. Ulanov, Alexander Li, Zhong Shaw, Lindsey N. Josefsson, Elisabet Horsburgh, Malcolm J. |
author_facet | Kenny, John G. Moran, Josephine Kolar, Stacey L. Ulanov, Alexander Li, Zhong Shaw, Lindsey N. Josefsson, Elisabet Horsburgh, Malcolm J. |
author_sort | Kenny, John G. |
collection | PubMed |
description | Mannitol (Mtl) fermentation, with the subsequent production of acid, is a species signature of Staphylococcus aureus, and discriminates it from most other members of the genus. Inactivation of the gene mtlD, encoding Mtl-1-P dehydrogenase was found to markedly reduce survival in the presence of the antimicrobial fatty acid, linoleic acid. We demonstrate that the sugar alcohol has a potentiating action for this membrane-acting antimicrobial. Analysis of cellular metabolites revealed that, during exponential growth, the mtlD mutant accumulated high levels of Mtl and Mtl-P. The latter metabolite was not detected in its isogenic parent strain or a deletion mutant of the entire mtlABFD operon. In addition, the mtlD mutant strain exhibited a decreased MIC for H(2)O(2), however virulence was unaffected in a model of septic arthritis. |
format | Online Article Text |
id | pubmed-3701532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37015322013-07-16 Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids Kenny, John G. Moran, Josephine Kolar, Stacey L. Ulanov, Alexander Li, Zhong Shaw, Lindsey N. Josefsson, Elisabet Horsburgh, Malcolm J. PLoS One Research Article Mannitol (Mtl) fermentation, with the subsequent production of acid, is a species signature of Staphylococcus aureus, and discriminates it from most other members of the genus. Inactivation of the gene mtlD, encoding Mtl-1-P dehydrogenase was found to markedly reduce survival in the presence of the antimicrobial fatty acid, linoleic acid. We demonstrate that the sugar alcohol has a potentiating action for this membrane-acting antimicrobial. Analysis of cellular metabolites revealed that, during exponential growth, the mtlD mutant accumulated high levels of Mtl and Mtl-P. The latter metabolite was not detected in its isogenic parent strain or a deletion mutant of the entire mtlABFD operon. In addition, the mtlD mutant strain exhibited a decreased MIC for H(2)O(2), however virulence was unaffected in a model of septic arthritis. Public Library of Science 2013-07-04 /pmc/articles/PMC3701532/ /pubmed/23861785 http://dx.doi.org/10.1371/journal.pone.0067698 Text en © 2013 Kenny et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kenny, John G. Moran, Josephine Kolar, Stacey L. Ulanov, Alexander Li, Zhong Shaw, Lindsey N. Josefsson, Elisabet Horsburgh, Malcolm J. Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids |
title | Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids |
title_full | Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids |
title_fullStr | Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids |
title_full_unstemmed | Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids |
title_short | Mannitol Utilisation is Required for Protection of Staphylococcus aureus from Human Skin Antimicrobial Fatty Acids |
title_sort | mannitol utilisation is required for protection of staphylococcus aureus from human skin antimicrobial fatty acids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701532/ https://www.ncbi.nlm.nih.gov/pubmed/23861785 http://dx.doi.org/10.1371/journal.pone.0067698 |
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