Preparation of 5′-O-(1-Thiotriphosphate)-Modified Oligonucleotides Using Polymerase-Endonuclease Amplification Reaction (PEAR)

Antisense oligonucleotides (ASODNs) have been widely used as an important tool for regulating gene expression, and developed into therapeutics. Natural ODNs are susceptible to nuclease degradation, nucleic acid analogues, however, have less side effects, stronger stability and more potent activities...

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Detalles Bibliográficos
Autores principales: Li, Biao, Dong, Shihua, Wu, Jiajun, Zhang, Jianye, Chen, Gang, Dong, Quanjiang, Zhu, Xinhong, Wang, Xiaolong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701678/
https://www.ncbi.nlm.nih.gov/pubmed/23861771
http://dx.doi.org/10.1371/journal.pone.0067558

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701678/
https://www.ncbi.nlm.nih.gov/pubmed/23861771
http://dx.doi.org/10.1371/journal.pone.0067558

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