Cargando…
Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I
Sexually reproducing organisms halve their cellular ploidy during gametogenesis by undergoing a specialized form of cell division known as meiosis. During meiosis, a single round of DNA replication is followed by two rounds of nuclear divisions (referred to as meiosis I and II). While sister kinetoc...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701701/ https://www.ncbi.nlm.nih.gov/pubmed/23861669 http://dx.doi.org/10.1371/journal.pgen.1003610 |
_version_ | 1782275696699113472 |
---|---|
author | Sarkar, Sourav Shenoy, Rajesh T. Dalgaard, Jacob Z. Newnham, Louise Hoffmann, Eva Millar, Jonathan B. A. Arumugam, Prakash |
author_facet | Sarkar, Sourav Shenoy, Rajesh T. Dalgaard, Jacob Z. Newnham, Louise Hoffmann, Eva Millar, Jonathan B. A. Arumugam, Prakash |
author_sort | Sarkar, Sourav |
collection | PubMed |
description | Sexually reproducing organisms halve their cellular ploidy during gametogenesis by undergoing a specialized form of cell division known as meiosis. During meiosis, a single round of DNA replication is followed by two rounds of nuclear divisions (referred to as meiosis I and II). While sister kinetochores bind to microtubules emanating from opposite spindle poles during mitosis, they bind to microtubules originating from the same spindle pole during meiosis I. This phenomenon is referred to as mono-orientation and is essential for setting up the reductional mode of chromosome segregation during meiosis I. In budding yeast, mono-orientation depends on a four component protein complex referred to as monopolin which consists of two nucleolar proteins Csm1 and Lrs4, meiosis-specific protein Mam1 of unknown function and casein kinase Hrr25. Monopolin complex binds to kinetochores during meiosis I and prevents bipolar attachments. Although monopolin associates with kinetochores during meiosis I, its binding site(s) on the kinetochore is not known and its mechanism of action has not been established. By carrying out an imaging-based screen we have found that the MIND complex, a component of the central kinetochore, is required for monopolin association with kinetochores during meiosis. Furthermore, we demonstrate that interaction of monopolin subunit Csm1 with the N-terminal domain of MIND complex subunit Dsn1, is essential for both the association of monopolin with kinetochores and for monopolar attachment of sister kinetochores during meiosis I. As such this provides the first functional evidence for a monopolin-binding site at the kinetochore. |
format | Online Article Text |
id | pubmed-3701701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37017012013-07-16 Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I Sarkar, Sourav Shenoy, Rajesh T. Dalgaard, Jacob Z. Newnham, Louise Hoffmann, Eva Millar, Jonathan B. A. Arumugam, Prakash PLoS Genet Research Article Sexually reproducing organisms halve their cellular ploidy during gametogenesis by undergoing a specialized form of cell division known as meiosis. During meiosis, a single round of DNA replication is followed by two rounds of nuclear divisions (referred to as meiosis I and II). While sister kinetochores bind to microtubules emanating from opposite spindle poles during mitosis, they bind to microtubules originating from the same spindle pole during meiosis I. This phenomenon is referred to as mono-orientation and is essential for setting up the reductional mode of chromosome segregation during meiosis I. In budding yeast, mono-orientation depends on a four component protein complex referred to as monopolin which consists of two nucleolar proteins Csm1 and Lrs4, meiosis-specific protein Mam1 of unknown function and casein kinase Hrr25. Monopolin complex binds to kinetochores during meiosis I and prevents bipolar attachments. Although monopolin associates with kinetochores during meiosis I, its binding site(s) on the kinetochore is not known and its mechanism of action has not been established. By carrying out an imaging-based screen we have found that the MIND complex, a component of the central kinetochore, is required for monopolin association with kinetochores during meiosis. Furthermore, we demonstrate that interaction of monopolin subunit Csm1 with the N-terminal domain of MIND complex subunit Dsn1, is essential for both the association of monopolin with kinetochores and for monopolar attachment of sister kinetochores during meiosis I. As such this provides the first functional evidence for a monopolin-binding site at the kinetochore. Public Library of Science 2013-07-04 /pmc/articles/PMC3701701/ /pubmed/23861669 http://dx.doi.org/10.1371/journal.pgen.1003610 Text en © 2013 Sarkar et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Sarkar, Sourav Shenoy, Rajesh T. Dalgaard, Jacob Z. Newnham, Louise Hoffmann, Eva Millar, Jonathan B. A. Arumugam, Prakash Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I |
title | Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I |
title_full | Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I |
title_fullStr | Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I |
title_full_unstemmed | Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I |
title_short | Monopolin Subunit Csm1 Associates with MIND Complex to Establish Monopolar Attachment of Sister Kinetochores at Meiosis I |
title_sort | monopolin subunit csm1 associates with mind complex to establish monopolar attachment of sister kinetochores at meiosis i |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701701/ https://www.ncbi.nlm.nih.gov/pubmed/23861669 http://dx.doi.org/10.1371/journal.pgen.1003610 |
work_keys_str_mv | AT sarkarsourav monopolinsubunitcsm1associateswithmindcomplextoestablishmonopolarattachmentofsisterkinetochoresatmeiosisi AT shenoyrajesht monopolinsubunitcsm1associateswithmindcomplextoestablishmonopolarattachmentofsisterkinetochoresatmeiosisi AT dalgaardjacobz monopolinsubunitcsm1associateswithmindcomplextoestablishmonopolarattachmentofsisterkinetochoresatmeiosisi AT newnhamlouise monopolinsubunitcsm1associateswithmindcomplextoestablishmonopolarattachmentofsisterkinetochoresatmeiosisi AT hoffmanneva monopolinsubunitcsm1associateswithmindcomplextoestablishmonopolarattachmentofsisterkinetochoresatmeiosisi AT millarjonathanba monopolinsubunitcsm1associateswithmindcomplextoestablishmonopolarattachmentofsisterkinetochoresatmeiosisi AT arumugamprakash monopolinsubunitcsm1associateswithmindcomplextoestablishmonopolarattachmentofsisterkinetochoresatmeiosisi |