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The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication

Influenza A NS1 and NS2 proteins are encoded by the RNA segment 8 of the viral genome. NS1 is a multifunctional protein and a virulence factor while NS2 is involved in nuclear export of viral ribonucleoprotein complexes. A yeast two-hybrid screening strategy was used to identify host factors support...

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Autores principales: de Chassey, Benoît, Aublin-Gex, Anne, Ruggieri, Alessia, Meyniel-Schicklin, Laurène, Pradezynski, Fabrine, Davoust, Nathalie, Chantier, Thibault, Tafforeau, Lionel, Mangeot, Philippe-Emmanuel, Ciancia, Claire, Perrin-Cocon, Laure, Bartenschlager, Ralf, André, Patrice, Lotteau, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701712/
https://www.ncbi.nlm.nih.gov/pubmed/23853584
http://dx.doi.org/10.1371/journal.ppat.1003440
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author de Chassey, Benoît
Aublin-Gex, Anne
Ruggieri, Alessia
Meyniel-Schicklin, Laurène
Pradezynski, Fabrine
Davoust, Nathalie
Chantier, Thibault
Tafforeau, Lionel
Mangeot, Philippe-Emmanuel
Ciancia, Claire
Perrin-Cocon, Laure
Bartenschlager, Ralf
André, Patrice
Lotteau, Vincent
author_facet de Chassey, Benoît
Aublin-Gex, Anne
Ruggieri, Alessia
Meyniel-Schicklin, Laurène
Pradezynski, Fabrine
Davoust, Nathalie
Chantier, Thibault
Tafforeau, Lionel
Mangeot, Philippe-Emmanuel
Ciancia, Claire
Perrin-Cocon, Laure
Bartenschlager, Ralf
André, Patrice
Lotteau, Vincent
author_sort de Chassey, Benoît
collection PubMed
description Influenza A NS1 and NS2 proteins are encoded by the RNA segment 8 of the viral genome. NS1 is a multifunctional protein and a virulence factor while NS2 is involved in nuclear export of viral ribonucleoprotein complexes. A yeast two-hybrid screening strategy was used to identify host factors supporting NS1 and NS2 functions. More than 560 interactions between 79 cellular proteins and NS1 and NS2 proteins from 9 different influenza virus strains have been identified. These interacting proteins are potentially involved in each step of the infectious process and their contribution to viral replication was tested by RNA interference. Validation of the relevance of these host cell proteins for the viral replication cycle revealed that 7 of the 79 NS1 and/or NS2-interacting proteins positively or negatively controlled virus replication. One of the main factors targeted by NS1 of all virus strains was double-stranded RNA binding domain protein family. In particular, adenosine deaminase acting on RNA 1 (ADAR1) appeared as a pro-viral host factor whose expression is necessary for optimal viral protein synthesis and replication. Surprisingly, ADAR1 also appeared as a pro-viral host factor for dengue virus replication and directly interacted with the viral NS3 protein. ADAR1 editing activity was enhanced by both viruses through dengue virus NS3 and influenza virus NS1 proteins, suggesting a similar virus-host co-evolution.
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spelling pubmed-37017122013-07-12 The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication de Chassey, Benoît Aublin-Gex, Anne Ruggieri, Alessia Meyniel-Schicklin, Laurène Pradezynski, Fabrine Davoust, Nathalie Chantier, Thibault Tafforeau, Lionel Mangeot, Philippe-Emmanuel Ciancia, Claire Perrin-Cocon, Laure Bartenschlager, Ralf André, Patrice Lotteau, Vincent PLoS Pathog Research Article Influenza A NS1 and NS2 proteins are encoded by the RNA segment 8 of the viral genome. NS1 is a multifunctional protein and a virulence factor while NS2 is involved in nuclear export of viral ribonucleoprotein complexes. A yeast two-hybrid screening strategy was used to identify host factors supporting NS1 and NS2 functions. More than 560 interactions between 79 cellular proteins and NS1 and NS2 proteins from 9 different influenza virus strains have been identified. These interacting proteins are potentially involved in each step of the infectious process and their contribution to viral replication was tested by RNA interference. Validation of the relevance of these host cell proteins for the viral replication cycle revealed that 7 of the 79 NS1 and/or NS2-interacting proteins positively or negatively controlled virus replication. One of the main factors targeted by NS1 of all virus strains was double-stranded RNA binding domain protein family. In particular, adenosine deaminase acting on RNA 1 (ADAR1) appeared as a pro-viral host factor whose expression is necessary for optimal viral protein synthesis and replication. Surprisingly, ADAR1 also appeared as a pro-viral host factor for dengue virus replication and directly interacted with the viral NS3 protein. ADAR1 editing activity was enhanced by both viruses through dengue virus NS3 and influenza virus NS1 proteins, suggesting a similar virus-host co-evolution. Public Library of Science 2013-07-04 /pmc/articles/PMC3701712/ /pubmed/23853584 http://dx.doi.org/10.1371/journal.ppat.1003440 Text en © 2013 de Chassey et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
de Chassey, Benoît
Aublin-Gex, Anne
Ruggieri, Alessia
Meyniel-Schicklin, Laurène
Pradezynski, Fabrine
Davoust, Nathalie
Chantier, Thibault
Tafforeau, Lionel
Mangeot, Philippe-Emmanuel
Ciancia, Claire
Perrin-Cocon, Laure
Bartenschlager, Ralf
André, Patrice
Lotteau, Vincent
The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication
title The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication
title_full The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication
title_fullStr The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication
title_full_unstemmed The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication
title_short The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication
title_sort interactomes of influenza virus ns1 and ns2 proteins identify new host factors and provide insights for adar1 playing a supportive role in virus replication
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701712/
https://www.ncbi.nlm.nih.gov/pubmed/23853584
http://dx.doi.org/10.1371/journal.ppat.1003440
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