Cargando…
In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
BACKGROUND: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue co...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Pathologists and The Korean Society for Cytopathology
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701819/ https://www.ncbi.nlm.nih.gov/pubmed/23837016 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.238 |
_version_ | 1782275710069506048 |
---|---|
author | Kim, Kyu Ho Choi, Suk Jin Choi, Yeon Il Kim, Lucia Park, In Suh Han, Jee Young Kim, Joon Mee Chu, Young Chae |
author_facet | Kim, Kyu Ho Choi, Suk Jin Choi, Yeon Il Kim, Lucia Park, In Suh Han, Jee Young Kim, Joon Mee Chu, Young Chae |
author_sort | Kim, Kyu Ho |
collection | PubMed |
description | BACKGROUND: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue cores can occurs during cutting of the TMAs, posing technical problems for in-house manual construction of high-density TMAs. We aimed to update the method for in-house manual TMA construction to improve the quality of high-density TMAs. METHODS: Blocks of agarose gel were subjected to the standard tissue processing and embedding procedure to prepare recipient agarose-paraffin blocks. The self-made tissue punches and recipient agarose-paraffin blocks were used to construct TMAs, which were completely melted and re-embedded in paraffin to make finished TMA blocks. RESULTS: The donor tissue cores were completely integrated into the surrounding paraffin of the recipient blocks. This method enabled us to construct high-density TMAs with significantly less section distortion or loss of tissue cores during microtomy. CONCLUSIONS: Simple and inexpensive construction of high-density and high-quality TMAs can be warranted by using paraffinized agarose gels as recipient blocks. |
format | Online Article Text |
id | pubmed-3701819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Korean Society of Pathologists and The Korean Society for Cytopathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-37018192013-07-08 In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks Kim, Kyu Ho Choi, Suk Jin Choi, Yeon Il Kim, Lucia Park, In Suh Han, Jee Young Kim, Joon Mee Chu, Young Chae Korean J Pathol Original Article BACKGROUND: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue cores can occurs during cutting of the TMAs, posing technical problems for in-house manual construction of high-density TMAs. We aimed to update the method for in-house manual TMA construction to improve the quality of high-density TMAs. METHODS: Blocks of agarose gel were subjected to the standard tissue processing and embedding procedure to prepare recipient agarose-paraffin blocks. The self-made tissue punches and recipient agarose-paraffin blocks were used to construct TMAs, which were completely melted and re-embedded in paraffin to make finished TMA blocks. RESULTS: The donor tissue cores were completely integrated into the surrounding paraffin of the recipient blocks. This method enabled us to construct high-density TMAs with significantly less section distortion or loss of tissue cores during microtomy. CONCLUSIONS: Simple and inexpensive construction of high-density and high-quality TMAs can be warranted by using paraffinized agarose gels as recipient blocks. The Korean Society of Pathologists and The Korean Society for Cytopathology 2013-06 2013-06-25 /pmc/articles/PMC3701819/ /pubmed/23837016 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.238 Text en © 2013 The Korean Society of Pathologists/The Korean Society for Cytopathology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Kyu Ho Choi, Suk Jin Choi, Yeon Il Kim, Lucia Park, In Suh Han, Jee Young Kim, Joon Mee Chu, Young Chae In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks |
title | In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks |
title_full | In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks |
title_fullStr | In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks |
title_full_unstemmed | In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks |
title_short | In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks |
title_sort | in-house manual construction of high-density and high-quality tissue microarrays by using homemade recipient agarose-paraffin blocks |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701819/ https://www.ncbi.nlm.nih.gov/pubmed/23837016 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.238 |
work_keys_str_mv | AT kimkyuho inhousemanualconstructionofhighdensityandhighqualitytissuemicroarraysbyusinghomemaderecipientagaroseparaffinblocks AT choisukjin inhousemanualconstructionofhighdensityandhighqualitytissuemicroarraysbyusinghomemaderecipientagaroseparaffinblocks AT choiyeonil inhousemanualconstructionofhighdensityandhighqualitytissuemicroarraysbyusinghomemaderecipientagaroseparaffinblocks AT kimlucia inhousemanualconstructionofhighdensityandhighqualitytissuemicroarraysbyusinghomemaderecipientagaroseparaffinblocks AT parkinsuh inhousemanualconstructionofhighdensityandhighqualitytissuemicroarraysbyusinghomemaderecipientagaroseparaffinblocks AT hanjeeyoung inhousemanualconstructionofhighdensityandhighqualitytissuemicroarraysbyusinghomemaderecipientagaroseparaffinblocks AT kimjoonmee inhousemanualconstructionofhighdensityandhighqualitytissuemicroarraysbyusinghomemaderecipientagaroseparaffinblocks AT chuyoungchae inhousemanualconstructionofhighdensityandhighqualitytissuemicroarraysbyusinghomemaderecipientagaroseparaffinblocks |