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In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks

BACKGROUND: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue co...

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Autores principales: Kim, Kyu Ho, Choi, Suk Jin, Choi, Yeon Il, Kim, Lucia, Park, In Suh, Han, Jee Young, Kim, Joon Mee, Chu, Young Chae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Pathologists and The Korean Society for Cytopathology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701819/
https://www.ncbi.nlm.nih.gov/pubmed/23837016
http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.238
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author Kim, Kyu Ho
Choi, Suk Jin
Choi, Yeon Il
Kim, Lucia
Park, In Suh
Han, Jee Young
Kim, Joon Mee
Chu, Young Chae
author_facet Kim, Kyu Ho
Choi, Suk Jin
Choi, Yeon Il
Kim, Lucia
Park, In Suh
Han, Jee Young
Kim, Joon Mee
Chu, Young Chae
author_sort Kim, Kyu Ho
collection PubMed
description BACKGROUND: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue cores can occurs during cutting of the TMAs, posing technical problems for in-house manual construction of high-density TMAs. We aimed to update the method for in-house manual TMA construction to improve the quality of high-density TMAs. METHODS: Blocks of agarose gel were subjected to the standard tissue processing and embedding procedure to prepare recipient agarose-paraffin blocks. The self-made tissue punches and recipient agarose-paraffin blocks were used to construct TMAs, which were completely melted and re-embedded in paraffin to make finished TMA blocks. RESULTS: The donor tissue cores were completely integrated into the surrounding paraffin of the recipient blocks. This method enabled us to construct high-density TMAs with significantly less section distortion or loss of tissue cores during microtomy. CONCLUSIONS: Simple and inexpensive construction of high-density and high-quality TMAs can be warranted by using paraffinized agarose gels as recipient blocks.
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spelling pubmed-37018192013-07-08 In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks Kim, Kyu Ho Choi, Suk Jin Choi, Yeon Il Kim, Lucia Park, In Suh Han, Jee Young Kim, Joon Mee Chu, Young Chae Korean J Pathol Original Article BACKGROUND: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue cores can occurs during cutting of the TMAs, posing technical problems for in-house manual construction of high-density TMAs. We aimed to update the method for in-house manual TMA construction to improve the quality of high-density TMAs. METHODS: Blocks of agarose gel were subjected to the standard tissue processing and embedding procedure to prepare recipient agarose-paraffin blocks. The self-made tissue punches and recipient agarose-paraffin blocks were used to construct TMAs, which were completely melted and re-embedded in paraffin to make finished TMA blocks. RESULTS: The donor tissue cores were completely integrated into the surrounding paraffin of the recipient blocks. This method enabled us to construct high-density TMAs with significantly less section distortion or loss of tissue cores during microtomy. CONCLUSIONS: Simple and inexpensive construction of high-density and high-quality TMAs can be warranted by using paraffinized agarose gels as recipient blocks. The Korean Society of Pathologists and The Korean Society for Cytopathology 2013-06 2013-06-25 /pmc/articles/PMC3701819/ /pubmed/23837016 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.238 Text en © 2013 The Korean Society of Pathologists/The Korean Society for Cytopathology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Kyu Ho
Choi, Suk Jin
Choi, Yeon Il
Kim, Lucia
Park, In Suh
Han, Jee Young
Kim, Joon Mee
Chu, Young Chae
In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
title In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
title_full In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
title_fullStr In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
title_full_unstemmed In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
title_short In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
title_sort in-house manual construction of high-density and high-quality tissue microarrays by using homemade recipient agarose-paraffin blocks
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701819/
https://www.ncbi.nlm.nih.gov/pubmed/23837016
http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.238
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