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Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath

BACKGROUND: The objective of this study was to evaluate a newly-developed EASYPREP liquid-based cytology method in cervicovaginal specimens and compare it with SurePath. METHODS: Cervicovaginal specimens were prospectively collected from 1,000 patients with EASYPREP and SurePath. The specimens were...

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Autores principales: Lee, Youn Soo, Gong, Gyungyub, Sohn, Jin Hee, Ryu, Ki Sung, Lee, Jung Hun, Khang, Shin Kwang, Cho, Kyung-Ja, Kim, Yong-Man, Kang, Chang Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Pathologists and The Korean Society for Cytopathology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701823/
https://www.ncbi.nlm.nih.gov/pubmed/23837020
http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.265
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author Lee, Youn Soo
Gong, Gyungyub
Sohn, Jin Hee
Ryu, Ki Sung
Lee, Jung Hun
Khang, Shin Kwang
Cho, Kyung-Ja
Kim, Yong-Man
Kang, Chang Suk
author_facet Lee, Youn Soo
Gong, Gyungyub
Sohn, Jin Hee
Ryu, Ki Sung
Lee, Jung Hun
Khang, Shin Kwang
Cho, Kyung-Ja
Kim, Yong-Man
Kang, Chang Suk
author_sort Lee, Youn Soo
collection PubMed
description BACKGROUND: The objective of this study was to evaluate a newly-developed EASYPREP liquid-based cytology method in cervicovaginal specimens and compare it with SurePath. METHODS: Cervicovaginal specimens were prospectively collected from 1,000 patients with EASYPREP and SurePath. The specimens were first collected by brushing for SurePath and second for EASYPREP. The specimens of both methods were diagnosed according to the Bethesda System. Additionally, we performed to REBA HPV-ID genotyping and sequencing analysis for human papillomavirus (HPV) on 249 specimens. RESULTS: EASYPREP and SurePath showed even distribution of cells and were equal in cellularity and staining quality. The diagnostic agreement between the two methods was 96.5%. Based on the standard of SurePath, the sensitivity, specificity, positive predictive value, and negative predictive value of EASYPREP were 90.7%, 99.2%, 94.8%, and 98.5%, respectively. The positivity of REBA HPV-ID was 49.4% and 95.1% in normal and abnormal cytological samples, respectively. The result of REBA HPV-ID had high concordance with sequencing analysis. CONCLUSIONS: EASYPREP provided comparable results to SurePath in the diagnosis and staining quality of cytology examinations and in HPV testing with REBA HPV-ID. EASYPREP could be another LBC method choice for the cervicovaginal specimens. Additionally, REBA HPV-ID may be a useful method for HPV genotyping.
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spelling pubmed-37018232013-07-08 Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath Lee, Youn Soo Gong, Gyungyub Sohn, Jin Hee Ryu, Ki Sung Lee, Jung Hun Khang, Shin Kwang Cho, Kyung-Ja Kim, Yong-Man Kang, Chang Suk Korean J Pathol Original Article BACKGROUND: The objective of this study was to evaluate a newly-developed EASYPREP liquid-based cytology method in cervicovaginal specimens and compare it with SurePath. METHODS: Cervicovaginal specimens were prospectively collected from 1,000 patients with EASYPREP and SurePath. The specimens were first collected by brushing for SurePath and second for EASYPREP. The specimens of both methods were diagnosed according to the Bethesda System. Additionally, we performed to REBA HPV-ID genotyping and sequencing analysis for human papillomavirus (HPV) on 249 specimens. RESULTS: EASYPREP and SurePath showed even distribution of cells and were equal in cellularity and staining quality. The diagnostic agreement between the two methods was 96.5%. Based on the standard of SurePath, the sensitivity, specificity, positive predictive value, and negative predictive value of EASYPREP were 90.7%, 99.2%, 94.8%, and 98.5%, respectively. The positivity of REBA HPV-ID was 49.4% and 95.1% in normal and abnormal cytological samples, respectively. The result of REBA HPV-ID had high concordance with sequencing analysis. CONCLUSIONS: EASYPREP provided comparable results to SurePath in the diagnosis and staining quality of cytology examinations and in HPV testing with REBA HPV-ID. EASYPREP could be another LBC method choice for the cervicovaginal specimens. Additionally, REBA HPV-ID may be a useful method for HPV genotyping. The Korean Society of Pathologists and The Korean Society for Cytopathology 2013-06 2013-06-25 /pmc/articles/PMC3701823/ /pubmed/23837020 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.265 Text en © 2013 The Korean Society of Pathologists/The Korean Society for Cytopathology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Youn Soo
Gong, Gyungyub
Sohn, Jin Hee
Ryu, Ki Sung
Lee, Jung Hun
Khang, Shin Kwang
Cho, Kyung-Ja
Kim, Yong-Man
Kang, Chang Suk
Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath
title Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath
title_full Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath
title_fullStr Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath
title_full_unstemmed Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath
title_short Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath
title_sort cytological evaluation and reba hpv-id hpv testing of newly developed liquid-based cytology, easyprep: comparison with surepath
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701823/
https://www.ncbi.nlm.nih.gov/pubmed/23837020
http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.265
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