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Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath
BACKGROUND: The objective of this study was to evaluate a newly-developed EASYPREP liquid-based cytology method in cervicovaginal specimens and compare it with SurePath. METHODS: Cervicovaginal specimens were prospectively collected from 1,000 patients with EASYPREP and SurePath. The specimens were...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Pathologists and The Korean Society for Cytopathology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701823/ https://www.ncbi.nlm.nih.gov/pubmed/23837020 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.265 |
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author | Lee, Youn Soo Gong, Gyungyub Sohn, Jin Hee Ryu, Ki Sung Lee, Jung Hun Khang, Shin Kwang Cho, Kyung-Ja Kim, Yong-Man Kang, Chang Suk |
author_facet | Lee, Youn Soo Gong, Gyungyub Sohn, Jin Hee Ryu, Ki Sung Lee, Jung Hun Khang, Shin Kwang Cho, Kyung-Ja Kim, Yong-Man Kang, Chang Suk |
author_sort | Lee, Youn Soo |
collection | PubMed |
description | BACKGROUND: The objective of this study was to evaluate a newly-developed EASYPREP liquid-based cytology method in cervicovaginal specimens and compare it with SurePath. METHODS: Cervicovaginal specimens were prospectively collected from 1,000 patients with EASYPREP and SurePath. The specimens were first collected by brushing for SurePath and second for EASYPREP. The specimens of both methods were diagnosed according to the Bethesda System. Additionally, we performed to REBA HPV-ID genotyping and sequencing analysis for human papillomavirus (HPV) on 249 specimens. RESULTS: EASYPREP and SurePath showed even distribution of cells and were equal in cellularity and staining quality. The diagnostic agreement between the two methods was 96.5%. Based on the standard of SurePath, the sensitivity, specificity, positive predictive value, and negative predictive value of EASYPREP were 90.7%, 99.2%, 94.8%, and 98.5%, respectively. The positivity of REBA HPV-ID was 49.4% and 95.1% in normal and abnormal cytological samples, respectively. The result of REBA HPV-ID had high concordance with sequencing analysis. CONCLUSIONS: EASYPREP provided comparable results to SurePath in the diagnosis and staining quality of cytology examinations and in HPV testing with REBA HPV-ID. EASYPREP could be another LBC method choice for the cervicovaginal specimens. Additionally, REBA HPV-ID may be a useful method for HPV genotyping. |
format | Online Article Text |
id | pubmed-3701823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Korean Society of Pathologists and The Korean Society for Cytopathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-37018232013-07-08 Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath Lee, Youn Soo Gong, Gyungyub Sohn, Jin Hee Ryu, Ki Sung Lee, Jung Hun Khang, Shin Kwang Cho, Kyung-Ja Kim, Yong-Man Kang, Chang Suk Korean J Pathol Original Article BACKGROUND: The objective of this study was to evaluate a newly-developed EASYPREP liquid-based cytology method in cervicovaginal specimens and compare it with SurePath. METHODS: Cervicovaginal specimens were prospectively collected from 1,000 patients with EASYPREP and SurePath. The specimens were first collected by brushing for SurePath and second for EASYPREP. The specimens of both methods were diagnosed according to the Bethesda System. Additionally, we performed to REBA HPV-ID genotyping and sequencing analysis for human papillomavirus (HPV) on 249 specimens. RESULTS: EASYPREP and SurePath showed even distribution of cells and were equal in cellularity and staining quality. The diagnostic agreement between the two methods was 96.5%. Based on the standard of SurePath, the sensitivity, specificity, positive predictive value, and negative predictive value of EASYPREP were 90.7%, 99.2%, 94.8%, and 98.5%, respectively. The positivity of REBA HPV-ID was 49.4% and 95.1% in normal and abnormal cytological samples, respectively. The result of REBA HPV-ID had high concordance with sequencing analysis. CONCLUSIONS: EASYPREP provided comparable results to SurePath in the diagnosis and staining quality of cytology examinations and in HPV testing with REBA HPV-ID. EASYPREP could be another LBC method choice for the cervicovaginal specimens. Additionally, REBA HPV-ID may be a useful method for HPV genotyping. The Korean Society of Pathologists and The Korean Society for Cytopathology 2013-06 2013-06-25 /pmc/articles/PMC3701823/ /pubmed/23837020 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.265 Text en © 2013 The Korean Society of Pathologists/The Korean Society for Cytopathology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Youn Soo Gong, Gyungyub Sohn, Jin Hee Ryu, Ki Sung Lee, Jung Hun Khang, Shin Kwang Cho, Kyung-Ja Kim, Yong-Man Kang, Chang Suk Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath |
title | Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath |
title_full | Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath |
title_fullStr | Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath |
title_full_unstemmed | Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath |
title_short | Cytological Evaluation and REBA HPV-ID HPV Testing of Newly Developed Liquid-Based Cytology, EASYPREP: Comparison with SurePath |
title_sort | cytological evaluation and reba hpv-id hpv testing of newly developed liquid-based cytology, easyprep: comparison with surepath |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701823/ https://www.ncbi.nlm.nih.gov/pubmed/23837020 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.3.265 |
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