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Molecular Characterization of the Circulating Strains of Vibrio cholerae during 2010 Cholera Outbreak in Nigeria

This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, borehole, sachet, stream, and tap) and disin...

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Autores principales: Oyedeji, Kolawole S., Niemogha, Mary-Theresa, Nwaokorie, Francisca O., Bamidele, Tajudeen A., Ochoga, Michael, Akinsinde, Kehinde A., Brai, Bartholomew I., Oladele, David, Omonigbehin, Emmanuel A., Bamidele, Moses, Fesobi, Toun W., Musa, Adesola Z., Adeneye, Adeniyi K., Smith, Stella I., Ujah, Innocent A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Centre for Diarrhoeal Disease Research, Bangladesh 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702338/
https://www.ncbi.nlm.nih.gov/pubmed/23930335
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author Oyedeji, Kolawole S.
Niemogha, Mary-Theresa
Nwaokorie, Francisca O.
Bamidele, Tajudeen A.
Ochoga, Michael
Akinsinde, Kehinde A.
Brai, Bartholomew I.
Oladele, David
Omonigbehin, Emmanuel A.
Bamidele, Moses
Fesobi, Toun W.
Musa, Adesola Z.
Adeneye, Adeniyi K.
Smith, Stella I.
Ujah, Innocent A.
author_facet Oyedeji, Kolawole S.
Niemogha, Mary-Theresa
Nwaokorie, Francisca O.
Bamidele, Tajudeen A.
Ochoga, Michael
Akinsinde, Kehinde A.
Brai, Bartholomew I.
Oladele, David
Omonigbehin, Emmanuel A.
Bamidele, Moses
Fesobi, Toun W.
Musa, Adesola Z.
Adeneye, Adeniyi K.
Smith, Stella I.
Ujah, Innocent A.
author_sort Oyedeji, Kolawole S.
collection PubMed
description This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, borehole, sachet, stream, and tap) and disinfectants (sodium hypochlorite) were collected from three states in the country. The samples were inoculated on thiosulphate-citrate bile salt-sucrose (TCBS), Cary-Blair transport medium and smeared on glass slides for direct examination. The Vibrio cholerae isolates were serotyped, biotyped, and characterized using PCR of the cytotoxin gene A (ctxA), wbeO1, and wbfO139 gene primer. Of the 201 samples screened, 96 were positive for V. cholerae O1 (48%), with 69 (72%) positive for ctxA gene. The results from this study showed that the circulating strains of cholera in Nigeria were of Ogawa serotype, also observed in other outbreaks in Nigeria (1991, 1992, and 1996). However, the strains were of the Classical biotype and were mainly (72%) ctxA gene-positive. This current investigation has confirmed the production of cholera toxin by the circulating strains, and this could be harnessed for possible cholera vaccine production in Nigeria.
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spelling pubmed-37023382013-07-24 Molecular Characterization of the Circulating Strains of Vibrio cholerae during 2010 Cholera Outbreak in Nigeria Oyedeji, Kolawole S. Niemogha, Mary-Theresa Nwaokorie, Francisca O. Bamidele, Tajudeen A. Ochoga, Michael Akinsinde, Kehinde A. Brai, Bartholomew I. Oladele, David Omonigbehin, Emmanuel A. Bamidele, Moses Fesobi, Toun W. Musa, Adesola Z. Adeneye, Adeniyi K. Smith, Stella I. Ujah, Innocent A. J Health Popul Nutr Original Papers This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, borehole, sachet, stream, and tap) and disinfectants (sodium hypochlorite) were collected from three states in the country. The samples were inoculated on thiosulphate-citrate bile salt-sucrose (TCBS), Cary-Blair transport medium and smeared on glass slides for direct examination. The Vibrio cholerae isolates were serotyped, biotyped, and characterized using PCR of the cytotoxin gene A (ctxA), wbeO1, and wbfO139 gene primer. Of the 201 samples screened, 96 were positive for V. cholerae O1 (48%), with 69 (72%) positive for ctxA gene. The results from this study showed that the circulating strains of cholera in Nigeria were of Ogawa serotype, also observed in other outbreaks in Nigeria (1991, 1992, and 1996). However, the strains were of the Classical biotype and were mainly (72%) ctxA gene-positive. This current investigation has confirmed the production of cholera toxin by the circulating strains, and this could be harnessed for possible cholera vaccine production in Nigeria. International Centre for Diarrhoeal Disease Research, Bangladesh 2013-06 /pmc/articles/PMC3702338/ /pubmed/23930335 Text en © INTERNATIONAL CENTRE FOR DIARRHOEAL DISEASE RESEARCH, BANGLADESH http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Papers
Oyedeji, Kolawole S.
Niemogha, Mary-Theresa
Nwaokorie, Francisca O.
Bamidele, Tajudeen A.
Ochoga, Michael
Akinsinde, Kehinde A.
Brai, Bartholomew I.
Oladele, David
Omonigbehin, Emmanuel A.
Bamidele, Moses
Fesobi, Toun W.
Musa, Adesola Z.
Adeneye, Adeniyi K.
Smith, Stella I.
Ujah, Innocent A.
Molecular Characterization of the Circulating Strains of Vibrio cholerae during 2010 Cholera Outbreak in Nigeria
title Molecular Characterization of the Circulating Strains of Vibrio cholerae during 2010 Cholera Outbreak in Nigeria
title_full Molecular Characterization of the Circulating Strains of Vibrio cholerae during 2010 Cholera Outbreak in Nigeria
title_fullStr Molecular Characterization of the Circulating Strains of Vibrio cholerae during 2010 Cholera Outbreak in Nigeria
title_full_unstemmed Molecular Characterization of the Circulating Strains of Vibrio cholerae during 2010 Cholera Outbreak in Nigeria
title_short Molecular Characterization of the Circulating Strains of Vibrio cholerae during 2010 Cholera Outbreak in Nigeria
title_sort molecular characterization of the circulating strains of vibrio cholerae during 2010 cholera outbreak in nigeria
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702338/
https://www.ncbi.nlm.nih.gov/pubmed/23930335
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