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A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression
The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their pa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702404/ https://www.ncbi.nlm.nih.gov/pubmed/23728293 http://dx.doi.org/10.1038/nsmb.2597 |
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author | Winkler, Andreas Heintz, Udo Lindner, Robert Reinstein, Jochen Shoeman, Robert L. Schlichting, Ilme |
author_facet | Winkler, Andreas Heintz, Udo Lindner, Robert Reinstein, Jochen Shoeman, Robert L. Schlichting, Ilme |
author_sort | Winkler, Andreas |
collection | PubMed |
description | The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the organism for using light energy. Illumination, however, enhances repression under semi-aerobic conditions. Here, we describe molecular details of two proteins involved in oxygen- and light-control of photosynthesis gene expression, the light-sensing anti-repressor AppA and the transcriptional repressor PpsR. We combine information from crystal structures of both proteins and their complex with hydrogen-deuterium exchange data to show that light-activation of AppA–PpsR(2) affects the PpsR effector region within the complex. DNA-binding studies demonstrate the formation of a light-sensitive ternary AppA–PpsR–DNA complex. Implications of these results for light- and oxygen-regulation are discussed, highlighting new insights into blue-light-mediated signal transduction. |
format | Online Article Text |
id | pubmed-3702404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-37024042014-01-01 A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression Winkler, Andreas Heintz, Udo Lindner, Robert Reinstein, Jochen Shoeman, Robert L. Schlichting, Ilme Nat Struct Mol Biol Article The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the organism for using light energy. Illumination, however, enhances repression under semi-aerobic conditions. Here, we describe molecular details of two proteins involved in oxygen- and light-control of photosynthesis gene expression, the light-sensing anti-repressor AppA and the transcriptional repressor PpsR. We combine information from crystal structures of both proteins and their complex with hydrogen-deuterium exchange data to show that light-activation of AppA–PpsR(2) affects the PpsR effector region within the complex. DNA-binding studies demonstrate the formation of a light-sensitive ternary AppA–PpsR–DNA complex. Implications of these results for light- and oxygen-regulation are discussed, highlighting new insights into blue-light-mediated signal transduction. 2013-06-02 2013-07 /pmc/articles/PMC3702404/ /pubmed/23728293 http://dx.doi.org/10.1038/nsmb.2597 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Winkler, Andreas Heintz, Udo Lindner, Robert Reinstein, Jochen Shoeman, Robert L. Schlichting, Ilme A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression |
title | A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression |
title_full | A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression |
title_fullStr | A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression |
title_full_unstemmed | A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression |
title_short | A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression |
title_sort | ternary appa–ppsr–dna complex mediates light-regulation of photosynthesis-related gene expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702404/ https://www.ncbi.nlm.nih.gov/pubmed/23728293 http://dx.doi.org/10.1038/nsmb.2597 |
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