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A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression

The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their pa...

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Autores principales: Winkler, Andreas, Heintz, Udo, Lindner, Robert, Reinstein, Jochen, Shoeman, Robert L., Schlichting, Ilme
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702404/
https://www.ncbi.nlm.nih.gov/pubmed/23728293
http://dx.doi.org/10.1038/nsmb.2597
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author Winkler, Andreas
Heintz, Udo
Lindner, Robert
Reinstein, Jochen
Shoeman, Robert L.
Schlichting, Ilme
author_facet Winkler, Andreas
Heintz, Udo
Lindner, Robert
Reinstein, Jochen
Shoeman, Robert L.
Schlichting, Ilme
author_sort Winkler, Andreas
collection PubMed
description The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the organism for using light energy. Illumination, however, enhances repression under semi-aerobic conditions. Here, we describe molecular details of two proteins involved in oxygen- and light-control of photosynthesis gene expression, the light-sensing anti-repressor AppA and the transcriptional repressor PpsR. We combine information from crystal structures of both proteins and their complex with hydrogen-deuterium exchange data to show that light-activation of AppA–PpsR(2) affects the PpsR effector region within the complex. DNA-binding studies demonstrate the formation of a light-sensitive ternary AppA–PpsR–DNA complex. Implications of these results for light- and oxygen-regulation are discussed, highlighting new insights into blue-light-mediated signal transduction.
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spelling pubmed-37024042014-01-01 A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression Winkler, Andreas Heintz, Udo Lindner, Robert Reinstein, Jochen Shoeman, Robert L. Schlichting, Ilme Nat Struct Mol Biol Article The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the organism for using light energy. Illumination, however, enhances repression under semi-aerobic conditions. Here, we describe molecular details of two proteins involved in oxygen- and light-control of photosynthesis gene expression, the light-sensing anti-repressor AppA and the transcriptional repressor PpsR. We combine information from crystal structures of both proteins and their complex with hydrogen-deuterium exchange data to show that light-activation of AppA–PpsR(2) affects the PpsR effector region within the complex. DNA-binding studies demonstrate the formation of a light-sensitive ternary AppA–PpsR–DNA complex. Implications of these results for light- and oxygen-regulation are discussed, highlighting new insights into blue-light-mediated signal transduction. 2013-06-02 2013-07 /pmc/articles/PMC3702404/ /pubmed/23728293 http://dx.doi.org/10.1038/nsmb.2597 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Winkler, Andreas
Heintz, Udo
Lindner, Robert
Reinstein, Jochen
Shoeman, Robert L.
Schlichting, Ilme
A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression
title A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression
title_full A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression
title_fullStr A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression
title_full_unstemmed A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression
title_short A ternary AppA–PpsR–DNA complex mediates light-regulation of photosynthesis-related gene expression
title_sort ternary appa–ppsr–dna complex mediates light-regulation of photosynthesis-related gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702404/
https://www.ncbi.nlm.nih.gov/pubmed/23728293
http://dx.doi.org/10.1038/nsmb.2597
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