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An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli
Expression of multiple heterologous genes in a dedicated host is a prerequisite for approaches in synthetic biology, spanning from the production of recombinant multiprotein complexes to the transfer of tailor-made metabolic pathways. Such attempts are often exacerbated, due in most cases to a lack...
Autores principales: | Schiffels, Johannes, Pinkenburg, Olaf, Schelden, Maximilian, Aboulnaga, El-Hussiny A. A., Baumann, Marcus E. M., Selmer, Thorsten |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702609/ https://www.ncbi.nlm.nih.gov/pubmed/23861944 http://dx.doi.org/10.1371/journal.pone.0068812 |
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