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Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients
BACKGROUND: The aim of this study was to simultaneously measure the concentrations of vascular endothelial growth factor (VEGF) and soluble VEGF receptor-1 (sVEGFR-1, also known as sFlt-1) in the aqueous humor of patients with non-proliferative diabetic retinopathy (NPDR) and to investigate whether...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703163/ https://www.ncbi.nlm.nih.gov/pubmed/23853629 |
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author | Javanmard, Shaghayegh Haghjooy Hasanpour, Zahra Abbaspoor, Zahra Naderian, Gholam A Jahanmard, Mehdi |
author_facet | Javanmard, Shaghayegh Haghjooy Hasanpour, Zahra Abbaspoor, Zahra Naderian, Gholam A Jahanmard, Mehdi |
author_sort | Javanmard, Shaghayegh Haghjooy |
collection | PubMed |
description | BACKGROUND: The aim of this study was to simultaneously measure the concentrations of vascular endothelial growth factor (VEGF) and soluble VEGF receptor-1 (sVEGFR-1, also known as sFlt-1) in the aqueous humor of patients with non-proliferative diabetic retinopathy (NPDR) and to investigate whether aqueous levels of vascular endothelial growth factor (VEGF) and VEGFR-1 are related to diabetic macular edema. MATERIALS AND METHODS: Aqueous humor was collected from 27 diabetic patients and 33 age- and sex-matched normoglycemic controls and analyzed for pro-angiogenic VEGF and angiogenic inhibitor VEGFR-1 by enzyme-linked immunosorbent assay (ELISA). The mean foveal thickness was measured by optical coherence tomography (OCT). RESULTS: There was no significant difference in the aqueous levels of VEGF in patients with NPDR compared with control subjects (P > 0.05), while the NPDR patients had significantly lower sVEGFR-1 in their aqueous humor. Furthermore, a significant (P < 0.01) positive correlation was observed between VEGF/sVEGFR-1 concentration and the mean foveal thickness measured on OCT. CONCLUSION: The results suggest that decreased chelating effect of sVEGFR-1 may be the preliminary event allowing VEGF to activate the proangiogenic endothelial cell state and to induce permeability. The imbalance between angiogenic agent (VEGF) and the antiangiogenic factors (sFlt-1), which is disturbed in the diabetic state, may determine the fate of diabetic macular edema. |
format | Online Article Text |
id | pubmed-3703163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37031632013-07-12 Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients Javanmard, Shaghayegh Haghjooy Hasanpour, Zahra Abbaspoor, Zahra Naderian, Gholam A Jahanmard, Mehdi J Res Med Sci Original Article BACKGROUND: The aim of this study was to simultaneously measure the concentrations of vascular endothelial growth factor (VEGF) and soluble VEGF receptor-1 (sVEGFR-1, also known as sFlt-1) in the aqueous humor of patients with non-proliferative diabetic retinopathy (NPDR) and to investigate whether aqueous levels of vascular endothelial growth factor (VEGF) and VEGFR-1 are related to diabetic macular edema. MATERIALS AND METHODS: Aqueous humor was collected from 27 diabetic patients and 33 age- and sex-matched normoglycemic controls and analyzed for pro-angiogenic VEGF and angiogenic inhibitor VEGFR-1 by enzyme-linked immunosorbent assay (ELISA). The mean foveal thickness was measured by optical coherence tomography (OCT). RESULTS: There was no significant difference in the aqueous levels of VEGF in patients with NPDR compared with control subjects (P > 0.05), while the NPDR patients had significantly lower sVEGFR-1 in their aqueous humor. Furthermore, a significant (P < 0.01) positive correlation was observed between VEGF/sVEGFR-1 concentration and the mean foveal thickness measured on OCT. CONCLUSION: The results suggest that decreased chelating effect of sVEGFR-1 may be the preliminary event allowing VEGF to activate the proangiogenic endothelial cell state and to induce permeability. The imbalance between angiogenic agent (VEGF) and the antiangiogenic factors (sFlt-1), which is disturbed in the diabetic state, may determine the fate of diabetic macular edema. Medknow Publications & Media Pvt Ltd 2012-12 /pmc/articles/PMC3703163/ /pubmed/23853629 Text en Copyright: © Journal of Research in Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Javanmard, Shaghayegh Haghjooy Hasanpour, Zahra Abbaspoor, Zahra Naderian, Gholam A Jahanmard, Mehdi Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients |
title | Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients |
title_full | Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients |
title_fullStr | Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients |
title_full_unstemmed | Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients |
title_short | Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients |
title_sort | aqueous concentrations of vegf and soluble vegf receptor-1 in diabetic retinopathy patients |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703163/ https://www.ncbi.nlm.nih.gov/pubmed/23853629 |
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