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Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients

BACKGROUND: The aim of this study was to simultaneously measure the concentrations of vascular endothelial growth factor (VEGF) and soluble VEGF receptor-1 (sVEGFR-1, also known as sFlt-1) in the aqueous humor of patients with non-proliferative diabetic retinopathy (NPDR) and to investigate whether...

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Autores principales: Javanmard, Shaghayegh Haghjooy, Hasanpour, Zahra, Abbaspoor, Zahra, Naderian, Gholam A, Jahanmard, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703163/
https://www.ncbi.nlm.nih.gov/pubmed/23853629
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author Javanmard, Shaghayegh Haghjooy
Hasanpour, Zahra
Abbaspoor, Zahra
Naderian, Gholam A
Jahanmard, Mehdi
author_facet Javanmard, Shaghayegh Haghjooy
Hasanpour, Zahra
Abbaspoor, Zahra
Naderian, Gholam A
Jahanmard, Mehdi
author_sort Javanmard, Shaghayegh Haghjooy
collection PubMed
description BACKGROUND: The aim of this study was to simultaneously measure the concentrations of vascular endothelial growth factor (VEGF) and soluble VEGF receptor-1 (sVEGFR-1, also known as sFlt-1) in the aqueous humor of patients with non-proliferative diabetic retinopathy (NPDR) and to investigate whether aqueous levels of vascular endothelial growth factor (VEGF) and VEGFR-1 are related to diabetic macular edema. MATERIALS AND METHODS: Aqueous humor was collected from 27 diabetic patients and 33 age- and sex-matched normoglycemic controls and analyzed for pro-angiogenic VEGF and angiogenic inhibitor VEGFR-1 by enzyme-linked immunosorbent assay (ELISA). The mean foveal thickness was measured by optical coherence tomography (OCT). RESULTS: There was no significant difference in the aqueous levels of VEGF in patients with NPDR compared with control subjects (P > 0.05), while the NPDR patients had significantly lower sVEGFR-1 in their aqueous humor. Furthermore, a significant (P < 0.01) positive correlation was observed between VEGF/sVEGFR-1 concentration and the mean foveal thickness measured on OCT. CONCLUSION: The results suggest that decreased chelating effect of sVEGFR-1 may be the preliminary event allowing VEGF to activate the proangiogenic endothelial cell state and to induce permeability. The imbalance between angiogenic agent (VEGF) and the antiangiogenic factors (sFlt-1), which is disturbed in the diabetic state, may determine the fate of diabetic macular edema.
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spelling pubmed-37031632013-07-12 Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients Javanmard, Shaghayegh Haghjooy Hasanpour, Zahra Abbaspoor, Zahra Naderian, Gholam A Jahanmard, Mehdi J Res Med Sci Original Article BACKGROUND: The aim of this study was to simultaneously measure the concentrations of vascular endothelial growth factor (VEGF) and soluble VEGF receptor-1 (sVEGFR-1, also known as sFlt-1) in the aqueous humor of patients with non-proliferative diabetic retinopathy (NPDR) and to investigate whether aqueous levels of vascular endothelial growth factor (VEGF) and VEGFR-1 are related to diabetic macular edema. MATERIALS AND METHODS: Aqueous humor was collected from 27 diabetic patients and 33 age- and sex-matched normoglycemic controls and analyzed for pro-angiogenic VEGF and angiogenic inhibitor VEGFR-1 by enzyme-linked immunosorbent assay (ELISA). The mean foveal thickness was measured by optical coherence tomography (OCT). RESULTS: There was no significant difference in the aqueous levels of VEGF in patients with NPDR compared with control subjects (P > 0.05), while the NPDR patients had significantly lower sVEGFR-1 in their aqueous humor. Furthermore, a significant (P < 0.01) positive correlation was observed between VEGF/sVEGFR-1 concentration and the mean foveal thickness measured on OCT. CONCLUSION: The results suggest that decreased chelating effect of sVEGFR-1 may be the preliminary event allowing VEGF to activate the proangiogenic endothelial cell state and to induce permeability. The imbalance between angiogenic agent (VEGF) and the antiangiogenic factors (sFlt-1), which is disturbed in the diabetic state, may determine the fate of diabetic macular edema. Medknow Publications & Media Pvt Ltd 2012-12 /pmc/articles/PMC3703163/ /pubmed/23853629 Text en Copyright: © Journal of Research in Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Javanmard, Shaghayegh Haghjooy
Hasanpour, Zahra
Abbaspoor, Zahra
Naderian, Gholam A
Jahanmard, Mehdi
Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients
title Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients
title_full Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients
title_fullStr Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients
title_full_unstemmed Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients
title_short Aqueous concentrations of VEGF and soluble VEGF receptor-1 in diabetic retinopathy patients
title_sort aqueous concentrations of vegf and soluble vegf receptor-1 in diabetic retinopathy patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703163/
https://www.ncbi.nlm.nih.gov/pubmed/23853629
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