Multiplex PCR for Rapid Diagnosis of Gastrointestinal Tuberculosis

BACKGROUND: Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance. AIM: To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB. MATERIALS AND METHODS: MPCR was performed on colonoscopy biopsy spe...

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Detalles Bibliográficos
Autores principales: Sharma, Kusum, Sinha, Saroj Kant, Sharma, Aman, Nada, Ritambra, Prasad, Kaushal K, Goyal, Kapil, Rana, Surinder Singh, Bhasin, Deepak Kumar, Sharma, Meera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703210/
https://www.ncbi.nlm.nih.gov/pubmed/23853431
http://dx.doi.org/10.4103/0974-777X.112272
Descripción
Sumario:BACKGROUND: Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance. AIM: To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB. MATERIALS AND METHODS: MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/histopathology was positive), 29 GITB suspected and 30 Non GITB (control group) patients. RESULTS: MPB64 PCR had sensitivity and specificity of 90% and 100% for confirmed GITB cases. In 29 clinically diagnosed but unconfirmed GITB cases, MPCR was positive in 72.41%. MPCR was negative in all control group patients. The overall sensitivity and specificity of microscopy, culture, histopathology and MPCR was 5%, 2% 20% and 77.5% and 100%, 100%, 100% and 100% respectively. CONCLUSION: MPCR has good sensitivity and specificity in diagnosing gastrointestinal tuberculosis.

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