Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients

The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal str...

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Detalles Bibliográficos
Autores principales: Jarzembowski, T., Daca, A., Witkowski, J., Rutkowski, B., Gołębiewska, J., Dębska-Ślizień, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703730/
https://www.ncbi.nlm.nih.gov/pubmed/23862151
http://dx.doi.org/10.1155/2013/687156
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author Jarzembowski, T.
Daca, A.
Witkowski, J.
Rutkowski, B.
Gołębiewska, J.
Dębska-Ślizień, A.
author_facet Jarzembowski, T.
Daca, A.
Witkowski, J.
Rutkowski, B.
Gołębiewska, J.
Dębska-Ślizień, A.
author_sort Jarzembowski, T.
collection PubMed
description The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal strains isolated from renal transplantation (RTx) recipients than in commensal strains. Additionally, in contrast to commensal strains in isolates from RTx patients, PBP5 gene expression was 17.45% higher in biofilms than in planktonic cells. Detailed comparison also showed that cyclosporine seemed to induce higher expression of PBP5 as compared to tacrolimus.
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spelling pubmed-37037302013-07-16 Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients Jarzembowski, T. Daca, A. Witkowski, J. Rutkowski, B. Gołębiewska, J. Dębska-Ślizień, A. Biomed Res Int Research Article The aim of the study was to evaluate changes in expression of PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe was used to measure resistance gene expression by the Flow-FISH method. Expression of the PBP5 gene measured by Flow-FISH was higher in enterococcal strains isolated from renal transplantation (RTx) recipients than in commensal strains. Additionally, in contrast to commensal strains in isolates from RTx patients, PBP5 gene expression was 17.45% higher in biofilms than in planktonic cells. Detailed comparison also showed that cyclosporine seemed to induce higher expression of PBP5 as compared to tacrolimus. Hindawi Publishing Corporation 2013 2013-06-19 /pmc/articles/PMC3703730/ /pubmed/23862151 http://dx.doi.org/10.1155/2013/687156 Text en Copyright © 2013 T. Jarzembowski et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jarzembowski, T.
Daca, A.
Witkowski, J.
Rutkowski, B.
Gołębiewska, J.
Dębska-Ślizień, A.
Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_full Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_fullStr Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_full_unstemmed Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_short Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients
title_sort changes of pbp5 gene expression in enterococcal isolates from renal transplantation recipients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703730/
https://www.ncbi.nlm.nih.gov/pubmed/23862151
http://dx.doi.org/10.1155/2013/687156
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