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Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus

Propolis is a natural product made by honeybees that has been widely used in folk medicine with a broad spectrum of biological activities. To investigate the antidepressant-like activity of propolis extract, CD-1 mice were administered an ethanol extract of propolis (50, 100, or 200 mg/kg, p.o.) pri...

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Autores principales: Lee, Mi-Sook, Kim, Young Han, Park, Wan-Soon, Ahn, Won Gyeong, Park, Ok Kyu, Kwon, Seung-Hae, Morita, Kyoji, Shim, Insop, Her, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703885/
https://www.ncbi.nlm.nih.gov/pubmed/23853655
http://dx.doi.org/10.1155/2013/217853
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author Lee, Mi-Sook
Kim, Young Han
Park, Wan-Soon
Ahn, Won Gyeong
Park, Ok Kyu
Kwon, Seung-Hae
Morita, Kyoji
Shim, Insop
Her, Song
author_facet Lee, Mi-Sook
Kim, Young Han
Park, Wan-Soon
Ahn, Won Gyeong
Park, Ok Kyu
Kwon, Seung-Hae
Morita, Kyoji
Shim, Insop
Her, Song
author_sort Lee, Mi-Sook
collection PubMed
description Propolis is a natural product made by honeybees that has been widely used in folk medicine with a broad spectrum of biological activities. To investigate the antidepressant-like activity of propolis extract, CD-1 mice were administered an ethanol extract of propolis (50, 100, or 200 mg/kg, p.o.) prior to the behavioral test. The propolis extract-treated group showed a dose-dependent decrease in immobility time in the FST and tail suspension test without altering locomotor activity. Propolis extract decreased the limbic hypothalamic-pituitary-adrenal axis response to the FST as indicated by an attenuated corticosterone response and decreased in c-fos immunoreactive neurons in the hippocampal dentate gyrus. Western blot analysis revealed a reduction in hippocampal glucocorticoid receptor (GR) expression following the FST, which was reversed by propolis extract. Propolis extract also increased pGR(S220)/(S234) ratio by a differential phosphorylation in S220 and S234. FST-induced downregulation of cAMP-responsive element binding protein phosphorylation at S133 (pCREB) was restored by propolis extract, showing a strong and positive relationship between pCREB and pGR(S220)/(S234) ratio. These findings suggest that the propolis extract potentiates antidepressant-like activity by enhancing GR function which is one of the therapeutic mechanisms of antidepressant; thus, propolis extract may provide a novel therapy for depression.
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spelling pubmed-37038852013-07-12 Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus Lee, Mi-Sook Kim, Young Han Park, Wan-Soon Ahn, Won Gyeong Park, Ok Kyu Kwon, Seung-Hae Morita, Kyoji Shim, Insop Her, Song Evid Based Complement Alternat Med Research Article Propolis is a natural product made by honeybees that has been widely used in folk medicine with a broad spectrum of biological activities. To investigate the antidepressant-like activity of propolis extract, CD-1 mice were administered an ethanol extract of propolis (50, 100, or 200 mg/kg, p.o.) prior to the behavioral test. The propolis extract-treated group showed a dose-dependent decrease in immobility time in the FST and tail suspension test without altering locomotor activity. Propolis extract decreased the limbic hypothalamic-pituitary-adrenal axis response to the FST as indicated by an attenuated corticosterone response and decreased in c-fos immunoreactive neurons in the hippocampal dentate gyrus. Western blot analysis revealed a reduction in hippocampal glucocorticoid receptor (GR) expression following the FST, which was reversed by propolis extract. Propolis extract also increased pGR(S220)/(S234) ratio by a differential phosphorylation in S220 and S234. FST-induced downregulation of cAMP-responsive element binding protein phosphorylation at S133 (pCREB) was restored by propolis extract, showing a strong and positive relationship between pCREB and pGR(S220)/(S234) ratio. These findings suggest that the propolis extract potentiates antidepressant-like activity by enhancing GR function which is one of the therapeutic mechanisms of antidepressant; thus, propolis extract may provide a novel therapy for depression. Hindawi Publishing Corporation 2013 2013-06-19 /pmc/articles/PMC3703885/ /pubmed/23853655 http://dx.doi.org/10.1155/2013/217853 Text en Copyright © 2013 Mi-Sook Lee et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lee, Mi-Sook
Kim, Young Han
Park, Wan-Soon
Ahn, Won Gyeong
Park, Ok Kyu
Kwon, Seung-Hae
Morita, Kyoji
Shim, Insop
Her, Song
Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus
title Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus
title_full Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus
title_fullStr Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus
title_full_unstemmed Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus
title_short Novel Antidepressant-Like Activity of Propolis Extract Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus
title_sort novel antidepressant-like activity of propolis extract mediated by enhanced glucocorticoid receptor function in the hippocampus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703885/
https://www.ncbi.nlm.nih.gov/pubmed/23853655
http://dx.doi.org/10.1155/2013/217853
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