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The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells
Intestinal microfold (M) cells are an enigmatic lineage of intestinal epithelial cells that initiate mucosal immune responses by uptake and transcytosis of luminal antigens. The mechanisms of M-cell differentiation are poorly understood as the rarity of these cells has hampered analysis. Exogenous R...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3704196/ https://www.ncbi.nlm.nih.gov/pubmed/22706340 http://dx.doi.org/10.1038/ni.2352 |
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| author | Kanaya, Takashi Hase, Koji Takahashi, Daisuke Fukuda, Shinji Hoshino, Katsuaki Sasaki, Izumi Hemmi, Hiroaki Knoop, Kathryn A Kumar, Nachiket Sato, Mayuko Katsuno, Tatsuro Yokosuka, Osamu Toyooka, Kiminori Nakai, Kumiko Sakamoto, Ayako Kitahara, Yuuki Jinnohara, Toshi McSorley, Stephen J Kaisho, Tsuneyasu Williams, Ifor R Ohno, Hiroshi |
| author_facet | Kanaya, Takashi Hase, Koji Takahashi, Daisuke Fukuda, Shinji Hoshino, Katsuaki Sasaki, Izumi Hemmi, Hiroaki Knoop, Kathryn A Kumar, Nachiket Sato, Mayuko Katsuno, Tatsuro Yokosuka, Osamu Toyooka, Kiminori Nakai, Kumiko Sakamoto, Ayako Kitahara, Yuuki Jinnohara, Toshi McSorley, Stephen J Kaisho, Tsuneyasu Williams, Ifor R Ohno, Hiroshi |
| author_sort | Kanaya, Takashi |
| collection | PubMed |
| description | Intestinal microfold (M) cells are an enigmatic lineage of intestinal epithelial cells that initiate mucosal immune responses by uptake and transcytosis of luminal antigens. The mechanisms of M-cell differentiation are poorly understood as the rarity of these cells has hampered analysis. Exogenous RANKL administration can synchronously activate M-cell differentiation in mice. Here we show the Ets transcription factor Spi-B was induced early during M-cell differentiation. Absence of Spi-B silenced the expression of multiple M-cell markers and prevented the differentiation of M cells in mice. Oral antigen-specific T cell activation was significantly impaired in the intestine of Spib(−/−) mice. Our study demonstrates that intestinal M-cell lineage commitment requires Spi-B as a candidate master regulator. |
| format | Online Article Text |
| id | pubmed-3704196 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2012 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-37041962013-07-08 The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells Kanaya, Takashi Hase, Koji Takahashi, Daisuke Fukuda, Shinji Hoshino, Katsuaki Sasaki, Izumi Hemmi, Hiroaki Knoop, Kathryn A Kumar, Nachiket Sato, Mayuko Katsuno, Tatsuro Yokosuka, Osamu Toyooka, Kiminori Nakai, Kumiko Sakamoto, Ayako Kitahara, Yuuki Jinnohara, Toshi McSorley, Stephen J Kaisho, Tsuneyasu Williams, Ifor R Ohno, Hiroshi Nat Immunol Article Intestinal microfold (M) cells are an enigmatic lineage of intestinal epithelial cells that initiate mucosal immune responses by uptake and transcytosis of luminal antigens. The mechanisms of M-cell differentiation are poorly understood as the rarity of these cells has hampered analysis. Exogenous RANKL administration can synchronously activate M-cell differentiation in mice. Here we show the Ets transcription factor Spi-B was induced early during M-cell differentiation. Absence of Spi-B silenced the expression of multiple M-cell markers and prevented the differentiation of M cells in mice. Oral antigen-specific T cell activation was significantly impaired in the intestine of Spib(−/−) mice. Our study demonstrates that intestinal M-cell lineage commitment requires Spi-B as a candidate master regulator. 2012-06-17 /pmc/articles/PMC3704196/ /pubmed/22706340 http://dx.doi.org/10.1038/ni.2352 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Kanaya, Takashi Hase, Koji Takahashi, Daisuke Fukuda, Shinji Hoshino, Katsuaki Sasaki, Izumi Hemmi, Hiroaki Knoop, Kathryn A Kumar, Nachiket Sato, Mayuko Katsuno, Tatsuro Yokosuka, Osamu Toyooka, Kiminori Nakai, Kumiko Sakamoto, Ayako Kitahara, Yuuki Jinnohara, Toshi McSorley, Stephen J Kaisho, Tsuneyasu Williams, Ifor R Ohno, Hiroshi The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells |
| title | The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells |
| title_full | The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells |
| title_fullStr | The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells |
| title_full_unstemmed | The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells |
| title_short | The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells |
| title_sort | ets transcription factor spi-b is essential for the differentiation of intestinal microfold (m) cells |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3704196/ https://www.ncbi.nlm.nih.gov/pubmed/22706340 http://dx.doi.org/10.1038/ni.2352 |
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