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Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains

The phenotypic and phylogenetic diversity of micro-algae capable of accumulating triacylglycerols provides a challenge for the accurate determination of biotechnological potential. High-yielding strains are needed to improve economic viability and their compositional information is required for opti...

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Autores principales: Slocombe, Stephen P., Zhang, QianYi, Black, Kenneth D., Day, John G., Stanley, Michele S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705143/
https://www.ncbi.nlm.nih.gov/pubmed/23853418
http://dx.doi.org/10.1007/s10811-012-9947-5
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author Slocombe, Stephen P.
Zhang, QianYi
Black, Kenneth D.
Day, John G.
Stanley, Michele S.
author_facet Slocombe, Stephen P.
Zhang, QianYi
Black, Kenneth D.
Day, John G.
Stanley, Michele S.
author_sort Slocombe, Stephen P.
collection PubMed
description The phenotypic and phylogenetic diversity of micro-algae capable of accumulating triacylglycerols provides a challenge for the accurate determination of biotechnological potential. High-yielding strains are needed to improve economic viability and their compositional information is required for optimizing biodiesel properties. To facilitate a high-throughput screening programme, a very rapid direct-derivatization procedure capable of extracting lyophilized material for GC analysis was compared with a scaled-down Folch-based method. This was carried out on ten micro-algal strains from 6 phyla where the more rapid direct-derivatization approach was found to provide a more reliable measure of yield. The modified Folch-based procedure was found to substantially underestimate oil yield in one Chlorella species (P < 0.01). In terms of fatty acid composition however, the Folch procedure proved to be slightly better in recovering polyunsaturated fatty acids, in six out of the ten strains. Therefore, direct-derivatization is recommended for rapid determination of yields in screening approaches but can provide slightly less compositional accuracy than solvent-based extraction methods.
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spelling pubmed-37051432013-07-11 Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains Slocombe, Stephen P. Zhang, QianYi Black, Kenneth D. Day, John G. Stanley, Michele S. J Appl Phycol Article The phenotypic and phylogenetic diversity of micro-algae capable of accumulating triacylglycerols provides a challenge for the accurate determination of biotechnological potential. High-yielding strains are needed to improve economic viability and their compositional information is required for optimizing biodiesel properties. To facilitate a high-throughput screening programme, a very rapid direct-derivatization procedure capable of extracting lyophilized material for GC analysis was compared with a scaled-down Folch-based method. This was carried out on ten micro-algal strains from 6 phyla where the more rapid direct-derivatization approach was found to provide a more reliable measure of yield. The modified Folch-based procedure was found to substantially underestimate oil yield in one Chlorella species (P < 0.01). In terms of fatty acid composition however, the Folch procedure proved to be slightly better in recovering polyunsaturated fatty acids, in six out of the ten strains. Therefore, direct-derivatization is recommended for rapid determination of yields in screening approaches but can provide slightly less compositional accuracy than solvent-based extraction methods. Springer Netherlands 2012-12-14 2013 /pmc/articles/PMC3705143/ /pubmed/23853418 http://dx.doi.org/10.1007/s10811-012-9947-5 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Slocombe, Stephen P.
Zhang, QianYi
Black, Kenneth D.
Day, John G.
Stanley, Michele S.
Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains
title Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains
title_full Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains
title_fullStr Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains
title_full_unstemmed Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains
title_short Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains
title_sort comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705143/
https://www.ncbi.nlm.nih.gov/pubmed/23853418
http://dx.doi.org/10.1007/s10811-012-9947-5
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