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In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)

Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are predominantly found in soils and peat bogs. In this study, we analyzed core (C)-bGDGTs after hydrolysis of polar fractions using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry and analyzed intact P-bGDGTs u...

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Autores principales: Zhang, Chuanlun L., Wang, Jinxiang, Dodsworth, Jeremy A., Williams, Amanda J., Zhu, Chun, Hinrichs, Kai-Uwe, Zheng, Fengfeng, Hedlund, Brian P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705189/
https://www.ncbi.nlm.nih.gov/pubmed/23847605
http://dx.doi.org/10.3389/fmicb.2013.00181
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author Zhang, Chuanlun L.
Wang, Jinxiang
Dodsworth, Jeremy A.
Williams, Amanda J.
Zhu, Chun
Hinrichs, Kai-Uwe
Zheng, Fengfeng
Hedlund, Brian P.
author_facet Zhang, Chuanlun L.
Wang, Jinxiang
Dodsworth, Jeremy A.
Williams, Amanda J.
Zhu, Chun
Hinrichs, Kai-Uwe
Zheng, Fengfeng
Hedlund, Brian P.
author_sort Zhang, Chuanlun L.
collection PubMed
description Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are predominantly found in soils and peat bogs. In this study, we analyzed core (C)-bGDGTs after hydrolysis of polar fractions using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry and analyzed intact P-bGDGTs using total lipid extract (TLE) without hydrolysis by liquid chromatography-electrospray ionization-multiple stage mass spectrometry. Our results show multiple lines of evidence for the production of bGDGTs in sediments and cellulolytic enrichments in a hot spring (62–86°C) in the Great Basin (USA). First, in situ cellulolytic enrichment led to an increase in the relative abundance of hydrolysis-derived P-bGDGTs over their C-bGDGT counterparts. Second, the hydrolysis-derived P- and C-bGDGT profiles in the hot spring were different from those of the surrounding soil samples; in particular, a monoglycosidic bGDGT Ib containing 13,16-dimethyloctacosane and one cyclopentane moiety was detected in the TLE but it was undetectable in surrounding soil samples even after sample enrichments. Third, previously published 16S rRNA gene pyrotag analysis from the same lignocellulose samples demonstrated the enrichment of thermophiles, rather than mesophiles, and total bGDGT abundance in cellulolytic enrichments correlated with the relative abundance of 16S rRNA gene pyrotags from thermophilic bacteria in the phyla Bacteroidetes, Dictyoglomi, EM3, and OP9 (“Atribacteria”). These observations conclusively demonstrate the production of bGDGTs in this hot spring; however, the identity of organisms that produce bGDGTs in the geothermal environment remains unclear.
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spelling pubmed-37051892013-07-11 In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA) Zhang, Chuanlun L. Wang, Jinxiang Dodsworth, Jeremy A. Williams, Amanda J. Zhu, Chun Hinrichs, Kai-Uwe Zheng, Fengfeng Hedlund, Brian P. Front Microbiol Microbiology Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are predominantly found in soils and peat bogs. In this study, we analyzed core (C)-bGDGTs after hydrolysis of polar fractions using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry and analyzed intact P-bGDGTs using total lipid extract (TLE) without hydrolysis by liquid chromatography-electrospray ionization-multiple stage mass spectrometry. Our results show multiple lines of evidence for the production of bGDGTs in sediments and cellulolytic enrichments in a hot spring (62–86°C) in the Great Basin (USA). First, in situ cellulolytic enrichment led to an increase in the relative abundance of hydrolysis-derived P-bGDGTs over their C-bGDGT counterparts. Second, the hydrolysis-derived P- and C-bGDGT profiles in the hot spring were different from those of the surrounding soil samples; in particular, a monoglycosidic bGDGT Ib containing 13,16-dimethyloctacosane and one cyclopentane moiety was detected in the TLE but it was undetectable in surrounding soil samples even after sample enrichments. Third, previously published 16S rRNA gene pyrotag analysis from the same lignocellulose samples demonstrated the enrichment of thermophiles, rather than mesophiles, and total bGDGT abundance in cellulolytic enrichments correlated with the relative abundance of 16S rRNA gene pyrotags from thermophilic bacteria in the phyla Bacteroidetes, Dictyoglomi, EM3, and OP9 (“Atribacteria”). These observations conclusively demonstrate the production of bGDGTs in this hot spring; however, the identity of organisms that produce bGDGTs in the geothermal environment remains unclear. Frontiers Media S.A. 2013-07-09 /pmc/articles/PMC3705189/ /pubmed/23847605 http://dx.doi.org/10.3389/fmicb.2013.00181 Text en Copyright © 2013 Zhang, Wang, Dodsworth, Williams, Zhu, Hinrichs, Zheng and Hedlund. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Microbiology
Zhang, Chuanlun L.
Wang, Jinxiang
Dodsworth, Jeremy A.
Williams, Amanda J.
Zhu, Chun
Hinrichs, Kai-Uwe
Zheng, Fengfeng
Hedlund, Brian P.
In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)
title In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)
title_full In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)
title_fullStr In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)
title_full_unstemmed In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)
title_short In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)
title_sort in situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (usa)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705189/
https://www.ncbi.nlm.nih.gov/pubmed/23847605
http://dx.doi.org/10.3389/fmicb.2013.00181
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