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Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit

The mechanism by which the foot-and-mouth disease virus (FMDV) initiates infection of cells is thought to involve the attachment of the viral capsid to host integrins on the surface of target cells. However, the role of integrins in FMDV infection still needs to be fully understood, although it has...

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Autores principales: Zhang, Yan, Sun, Yingjun, Yang, Fan, Guo, Jianhong, He, Jijun, Wu, Qiong, Cao, Weijun, Lv, Lv, Zheng, Haixue, Zhang, Zhidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705268/
https://www.ncbi.nlm.nih.gov/pubmed/23604096
http://dx.doi.org/10.3390/v5041114
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author Zhang, Yan
Sun, Yingjun
Yang, Fan
Guo, Jianhong
He, Jijun
Wu, Qiong
Cao, Weijun
Lv, Lv
Zheng, Haixue
Zhang, Zhidong
author_facet Zhang, Yan
Sun, Yingjun
Yang, Fan
Guo, Jianhong
He, Jijun
Wu, Qiong
Cao, Weijun
Lv, Lv
Zheng, Haixue
Zhang, Zhidong
author_sort Zhang, Yan
collection PubMed
description The mechanism by which the foot-and-mouth disease virus (FMDV) initiates infection of cells is thought to involve the attachment of the viral capsid to host integrins on the surface of target cells. However, the role of integrins in FMDV infection still needs to be fully understood, although it has been demonstrated that integrin αvβ6 interferes with FMDV in vitro and results in neutralization of its infectivity. In the present study, we describe the cloning and sequencing of suckling mouse integrin β6 and the subsequent expression of two segments of integrin β6 extracellular domains: β6-1 (which contains the ligand-binding domain) and β6-2. Sequencing of the mouse integrin β6 subunit revealed close homology (~90%) with its human counterpart. When recombinant integrin extracellular domains β6-1 and β6-2 formulated with adjuvant were inoculated into guinea pigs, anti-integrin antibody expression was high before FMDV challenge. Interestingly, guinea pigs (50%) inoculated with integrin β6-1 were protected from FMDV infection; in contrast, none of the animals inoculated with integrin β6-2 were protected. This result indicates that an integrin blockade may be able to interfere with FMDV infection in vivo, which raises the possibility that targeting integrin in vivo may be the basis for a new strategy to control FMDV infection.
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spelling pubmed-37052682013-07-09 Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit Zhang, Yan Sun, Yingjun Yang, Fan Guo, Jianhong He, Jijun Wu, Qiong Cao, Weijun Lv, Lv Zheng, Haixue Zhang, Zhidong Viruses Article The mechanism by which the foot-and-mouth disease virus (FMDV) initiates infection of cells is thought to involve the attachment of the viral capsid to host integrins on the surface of target cells. However, the role of integrins in FMDV infection still needs to be fully understood, although it has been demonstrated that integrin αvβ6 interferes with FMDV in vitro and results in neutralization of its infectivity. In the present study, we describe the cloning and sequencing of suckling mouse integrin β6 and the subsequent expression of two segments of integrin β6 extracellular domains: β6-1 (which contains the ligand-binding domain) and β6-2. Sequencing of the mouse integrin β6 subunit revealed close homology (~90%) with its human counterpart. When recombinant integrin extracellular domains β6-1 and β6-2 formulated with adjuvant were inoculated into guinea pigs, anti-integrin antibody expression was high before FMDV challenge. Interestingly, guinea pigs (50%) inoculated with integrin β6-1 were protected from FMDV infection; in contrast, none of the animals inoculated with integrin β6-2 were protected. This result indicates that an integrin blockade may be able to interfere with FMDV infection in vivo, which raises the possibility that targeting integrin in vivo may be the basis for a new strategy to control FMDV infection. MDPI 2013-04-19 /pmc/articles/PMC3705268/ /pubmed/23604096 http://dx.doi.org/10.3390/v5041114 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Yan
Sun, Yingjun
Yang, Fan
Guo, Jianhong
He, Jijun
Wu, Qiong
Cao, Weijun
Lv, Lv
Zheng, Haixue
Zhang, Zhidong
Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit
title Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit
title_full Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit
title_fullStr Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit
title_full_unstemmed Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit
title_short Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin β6-1 Subunit
title_sort induction of partial protection against foot and mouth disease virus in guinea pigs by neutralization with the integrin β6-1 subunit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705268/
https://www.ncbi.nlm.nih.gov/pubmed/23604096
http://dx.doi.org/10.3390/v5041114
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