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Foamy Virus Assembly with Emphasis on Pol Encapsidation
Foamy viruses (FVs) differ from all other genera of retroviruses (orthoretroviruses) in many aspects of viral replication. In this review, we discuss FV assembly, with special emphasis on Pol incorporation. FV assembly takes place intracellularly, near the pericentriolar region, at a site similar to...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705302/ https://www.ncbi.nlm.nih.gov/pubmed/23518575 http://dx.doi.org/10.3390/v5030886 |
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author | Lee, Eun-Gyung Stenbak, Carolyn R. Linial, Maxine L. |
author_facet | Lee, Eun-Gyung Stenbak, Carolyn R. Linial, Maxine L. |
author_sort | Lee, Eun-Gyung |
collection | PubMed |
description | Foamy viruses (FVs) differ from all other genera of retroviruses (orthoretroviruses) in many aspects of viral replication. In this review, we discuss FV assembly, with special emphasis on Pol incorporation. FV assembly takes place intracellularly, near the pericentriolar region, at a site similar to that used by betaretroviruses. The regions of Gag, Pol and genomic RNA required for viral assembly are described. In contrast to orthoretroviral Pol, which is synthesized as a Gag-Pol fusion protein and packaged through Gag-Gag interactions, FV Pol is synthesized from a spliced mRNA lacking all Gag sequences. Thus, encapsidation of FV Pol requires a different mechanism. We detail how WT Pol lacking Gag sequences is incorporated into virus particles. In addition, a mutant in which Pol is expressed as an orthoretroviral-like Gag-Pol fusion protein is discussed. We also discuss temporal regulation of the protease, reverse transcriptase and integrase activities of WT FV Pol. |
format | Online Article Text |
id | pubmed-3705302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-37053022013-07-09 Foamy Virus Assembly with Emphasis on Pol Encapsidation Lee, Eun-Gyung Stenbak, Carolyn R. Linial, Maxine L. Viruses Review Foamy viruses (FVs) differ from all other genera of retroviruses (orthoretroviruses) in many aspects of viral replication. In this review, we discuss FV assembly, with special emphasis on Pol incorporation. FV assembly takes place intracellularly, near the pericentriolar region, at a site similar to that used by betaretroviruses. The regions of Gag, Pol and genomic RNA required for viral assembly are described. In contrast to orthoretroviral Pol, which is synthesized as a Gag-Pol fusion protein and packaged through Gag-Gag interactions, FV Pol is synthesized from a spliced mRNA lacking all Gag sequences. Thus, encapsidation of FV Pol requires a different mechanism. We detail how WT Pol lacking Gag sequences is incorporated into virus particles. In addition, a mutant in which Pol is expressed as an orthoretroviral-like Gag-Pol fusion protein is discussed. We also discuss temporal regulation of the protease, reverse transcriptase and integrase activities of WT FV Pol. MDPI 2013-03-20 /pmc/articles/PMC3705302/ /pubmed/23518575 http://dx.doi.org/10.3390/v5030886 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Lee, Eun-Gyung Stenbak, Carolyn R. Linial, Maxine L. Foamy Virus Assembly with Emphasis on Pol Encapsidation |
title | Foamy Virus Assembly with Emphasis on Pol Encapsidation |
title_full | Foamy Virus Assembly with Emphasis on Pol Encapsidation |
title_fullStr | Foamy Virus Assembly with Emphasis on Pol Encapsidation |
title_full_unstemmed | Foamy Virus Assembly with Emphasis on Pol Encapsidation |
title_short | Foamy Virus Assembly with Emphasis on Pol Encapsidation |
title_sort | foamy virus assembly with emphasis on pol encapsidation |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705302/ https://www.ncbi.nlm.nih.gov/pubmed/23518575 http://dx.doi.org/10.3390/v5030886 |
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