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Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin

Forty four marine actinomycetes of the family Microccocaceae isolated from sponges collected primarily in Florida Keys (USA) were selected from our strain collection to be studied as new sources for the production of bioactive natural products. A 16S rRNA gene based phylogenetic analysis showed that...

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Autores principales: Palomo, Sara, González, Ignacio, de la Cruz, Mercedes, Martín, Jesús, Tormo, José Rubén, Anderson, Matthew, Hill, Russell T., Vicente, Francisca, Reyes, Fernando, Genilloud, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705389/
https://www.ncbi.nlm.nih.gov/pubmed/23538871
http://dx.doi.org/10.3390/md11041071
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author Palomo, Sara
González, Ignacio
de la Cruz, Mercedes
Martín, Jesús
Tormo, José Rubén
Anderson, Matthew
Hill, Russell T.
Vicente, Francisca
Reyes, Fernando
Genilloud, Olga
author_facet Palomo, Sara
González, Ignacio
de la Cruz, Mercedes
Martín, Jesús
Tormo, José Rubén
Anderson, Matthew
Hill, Russell T.
Vicente, Francisca
Reyes, Fernando
Genilloud, Olga
author_sort Palomo, Sara
collection PubMed
description Forty four marine actinomycetes of the family Microccocaceae isolated from sponges collected primarily in Florida Keys (USA) were selected from our strain collection to be studied as new sources for the production of bioactive natural products. A 16S rRNA gene based phylogenetic analysis showed that the strains are members of the genera Kocuria and Micrococcus. To assess their biosynthetic potential, the strains were PCR screened for the presence of secondary metabolite genes encoding nonribosomal synthetase (NRPS) and polyketide synthases (PKS). A small extract collection of 528 crude extracts generated from nutritional microfermentation arrays was tested for the production of bioactive secondary metabolites against clinically relevant strains (Bacillus subtilis, methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumannii and Candida albicans). Three independent isolates were shown to produce a new anti-MRSA bioactive compound that was identified as kocurin, a new member of the thiazolyl peptide family of antibiotics emphasizing the role of this family as a prolific resource for novel drugs.
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spelling pubmed-37053892013-07-09 Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin Palomo, Sara González, Ignacio de la Cruz, Mercedes Martín, Jesús Tormo, José Rubén Anderson, Matthew Hill, Russell T. Vicente, Francisca Reyes, Fernando Genilloud, Olga Mar Drugs Article Forty four marine actinomycetes of the family Microccocaceae isolated from sponges collected primarily in Florida Keys (USA) were selected from our strain collection to be studied as new sources for the production of bioactive natural products. A 16S rRNA gene based phylogenetic analysis showed that the strains are members of the genera Kocuria and Micrococcus. To assess their biosynthetic potential, the strains were PCR screened for the presence of secondary metabolite genes encoding nonribosomal synthetase (NRPS) and polyketide synthases (PKS). A small extract collection of 528 crude extracts generated from nutritional microfermentation arrays was tested for the production of bioactive secondary metabolites against clinically relevant strains (Bacillus subtilis, methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumannii and Candida albicans). Three independent isolates were shown to produce a new anti-MRSA bioactive compound that was identified as kocurin, a new member of the thiazolyl peptide family of antibiotics emphasizing the role of this family as a prolific resource for novel drugs. MDPI 2013-03-28 /pmc/articles/PMC3705389/ /pubmed/23538871 http://dx.doi.org/10.3390/md11041071 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Palomo, Sara
González, Ignacio
de la Cruz, Mercedes
Martín, Jesús
Tormo, José Rubén
Anderson, Matthew
Hill, Russell T.
Vicente, Francisca
Reyes, Fernando
Genilloud, Olga
Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin
title Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin
title_full Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin
title_fullStr Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin
title_full_unstemmed Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin
title_short Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin
title_sort sponge-derived kocuria and micrococcus spp. as sources of the new thiazolyl peptide antibiotic kocurin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705389/
https://www.ncbi.nlm.nih.gov/pubmed/23538871
http://dx.doi.org/10.3390/md11041071
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