Cargando…

Differential in Gel Electrophoresis (DIGE) Comparative Proteomic Analysis of Macrophages Cell Cultures in Response to Perthamide C Treatment

Secondary metabolites contained in marine organisms disclose diverse pharmacological activities, due to their intrinsic ability to recognize bio-macromolecules, which alter their expression and modulate their function. Thus, the identification of the cellular pathways affected by marine natural prod...

Descripción completa

Detalles Bibliográficos
Autores principales: Vilasi, Annalisa, Monti, Maria Chiara, Tosco, Alessandra, De Marino, Simona, Margarucci, Luigi, Riccio, Raffaele, Casapullo, Agostino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705404/
https://www.ncbi.nlm.nih.gov/pubmed/23595056
http://dx.doi.org/10.3390/md11041288
_version_ 1782476435649200128
author Vilasi, Annalisa
Monti, Maria Chiara
Tosco, Alessandra
De Marino, Simona
Margarucci, Luigi
Riccio, Raffaele
Casapullo, Agostino
author_facet Vilasi, Annalisa
Monti, Maria Chiara
Tosco, Alessandra
De Marino, Simona
Margarucci, Luigi
Riccio, Raffaele
Casapullo, Agostino
author_sort Vilasi, Annalisa
collection PubMed
description Secondary metabolites contained in marine organisms disclose diverse pharmacological activities, due to their intrinsic ability to recognize bio-macromolecules, which alter their expression and modulate their function. Thus, the identification of the cellular pathways affected by marine natural products is crucial to provide important functional information concerning their mechanism of action at the molecular level. Perthamide C, a marine sponge metabolite isolated from the polar extracts of Theonella swinhoei and endowed with a broad and interesting anti-inflammatory profile, was found in a previous study to specifically interact with heat shock protein-90 and glucose regulated protein-94, also disclosing the ability to reduce cisplatin-mediated apoptosis. In this paper, we evaluated the effect of this compound on the whole proteome of murine macrophages cells by two-dimensional DIGE proteomics. Thirty-three spots were found to be altered in expression by at least 1.6-fold and 29 proteins were identified by LC ESI-Q/TOF-MS. These proteins are involved in different processes, such as metabolism, structural stability, protein folding assistance and gene expression. Among them, perthamide C modulates the expression of several chaperones implicated in the folding of proteins correlated to apoptosis, such as Hsp90 and T-complexes, and in this context our data shed more light on the cellular effects and pathways altered by this marine cyclo-peptide.
format Online
Article
Text
id pubmed-3705404
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-37054042013-07-09 Differential in Gel Electrophoresis (DIGE) Comparative Proteomic Analysis of Macrophages Cell Cultures in Response to Perthamide C Treatment Vilasi, Annalisa Monti, Maria Chiara Tosco, Alessandra De Marino, Simona Margarucci, Luigi Riccio, Raffaele Casapullo, Agostino Mar Drugs Article Secondary metabolites contained in marine organisms disclose diverse pharmacological activities, due to their intrinsic ability to recognize bio-macromolecules, which alter their expression and modulate their function. Thus, the identification of the cellular pathways affected by marine natural products is crucial to provide important functional information concerning their mechanism of action at the molecular level. Perthamide C, a marine sponge metabolite isolated from the polar extracts of Theonella swinhoei and endowed with a broad and interesting anti-inflammatory profile, was found in a previous study to specifically interact with heat shock protein-90 and glucose regulated protein-94, also disclosing the ability to reduce cisplatin-mediated apoptosis. In this paper, we evaluated the effect of this compound on the whole proteome of murine macrophages cells by two-dimensional DIGE proteomics. Thirty-three spots were found to be altered in expression by at least 1.6-fold and 29 proteins were identified by LC ESI-Q/TOF-MS. These proteins are involved in different processes, such as metabolism, structural stability, protein folding assistance and gene expression. Among them, perthamide C modulates the expression of several chaperones implicated in the folding of proteins correlated to apoptosis, such as Hsp90 and T-complexes, and in this context our data shed more light on the cellular effects and pathways altered by this marine cyclo-peptide. MDPI 2013-04-17 /pmc/articles/PMC3705404/ /pubmed/23595056 http://dx.doi.org/10.3390/md11041288 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Vilasi, Annalisa
Monti, Maria Chiara
Tosco, Alessandra
De Marino, Simona
Margarucci, Luigi
Riccio, Raffaele
Casapullo, Agostino
Differential in Gel Electrophoresis (DIGE) Comparative Proteomic Analysis of Macrophages Cell Cultures in Response to Perthamide C Treatment
title Differential in Gel Electrophoresis (DIGE) Comparative Proteomic Analysis of Macrophages Cell Cultures in Response to Perthamide C Treatment
title_full Differential in Gel Electrophoresis (DIGE) Comparative Proteomic Analysis of Macrophages Cell Cultures in Response to Perthamide C Treatment
title_fullStr Differential in Gel Electrophoresis (DIGE) Comparative Proteomic Analysis of Macrophages Cell Cultures in Response to Perthamide C Treatment
title_full_unstemmed Differential in Gel Electrophoresis (DIGE) Comparative Proteomic Analysis of Macrophages Cell Cultures in Response to Perthamide C Treatment
title_short Differential in Gel Electrophoresis (DIGE) Comparative Proteomic Analysis of Macrophages Cell Cultures in Response to Perthamide C Treatment
title_sort differential in gel electrophoresis (dige) comparative proteomic analysis of macrophages cell cultures in response to perthamide c treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705404/
https://www.ncbi.nlm.nih.gov/pubmed/23595056
http://dx.doi.org/10.3390/md11041288
work_keys_str_mv AT vilasiannalisa differentialingelelectrophoresisdigecomparativeproteomicanalysisofmacrophagescellculturesinresponsetoperthamidectreatment
AT montimariachiara differentialingelelectrophoresisdigecomparativeproteomicanalysisofmacrophagescellculturesinresponsetoperthamidectreatment
AT toscoalessandra differentialingelelectrophoresisdigecomparativeproteomicanalysisofmacrophagescellculturesinresponsetoperthamidectreatment
AT demarinosimona differentialingelelectrophoresisdigecomparativeproteomicanalysisofmacrophagescellculturesinresponsetoperthamidectreatment
AT margarucciluigi differentialingelelectrophoresisdigecomparativeproteomicanalysisofmacrophagescellculturesinresponsetoperthamidectreatment
AT riccioraffaele differentialingelelectrophoresisdigecomparativeproteomicanalysisofmacrophagescellculturesinresponsetoperthamidectreatment
AT casapulloagostino differentialingelelectrophoresisdigecomparativeproteomicanalysisofmacrophagescellculturesinresponsetoperthamidectreatment