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An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H(...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Molecular Diversity Preservation International (MDPI)
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705516/ https://www.ncbi.nlm.nih.gov/pubmed/27873826 http://dx.doi.org/10.3390/s8095492 |
| Sumario: | A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H(2)O(2) at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10(-6) × 8.5×10(-5) M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 °C, respectively. The stability and reproducibility of the enzyme electrode have been also studied. |
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