An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film

A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H(...

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Autor principal: Arslan, Fatma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705516/
https://www.ncbi.nlm.nih.gov/pubmed/27873826
http://dx.doi.org/10.3390/s8095492
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author Arslan, Fatma
author_facet Arslan, Fatma
author_sort Arslan, Fatma
collection PubMed
description A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H(2)O(2) at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10(-6) × 8.5×10(-5) M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 °C, respectively. The stability and reproducibility of the enzyme electrode have been also studied.
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spelling pubmed-37055162013-07-09 An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film Arslan, Fatma Sensors (Basel) Article A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H(2)O(2) at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5×10(-6) × 8.5×10(-5) M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 °C, respectively. The stability and reproducibility of the enzyme electrode have been also studied. Molecular Diversity Preservation International (MDPI) 2008-09-04 /pmc/articles/PMC3705516/ /pubmed/27873826 http://dx.doi.org/10.3390/s8095492 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Arslan, Fatma
An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
title An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
title_full An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
title_fullStr An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
title_full_unstemmed An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
title_short An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole Film
title_sort amperometric biosensor for uric acid determination prepared from uricase immobilized in polyaniline-polypyrrole film
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705516/
https://www.ncbi.nlm.nih.gov/pubmed/27873826
http://dx.doi.org/10.3390/s8095492
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