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Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces

Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity meas...

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Detalles Bibliográficos
Autores principales: Libertino, Sebania, Aiello, Venera, Scandurra, Antonino, Renis, Marcella, Sinatra, Fulvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705523/
https://www.ncbi.nlm.nih.gov/pubmed/27873833
http://dx.doi.org/10.3390/s8095637
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author Libertino, Sebania
Aiello, Venera
Scandurra, Antonino
Renis, Marcella
Sinatra, Fulvia
author_facet Libertino, Sebania
Aiello, Venera
Scandurra, Antonino
Renis, Marcella
Sinatra, Fulvia
author_sort Libertino, Sebania
collection PubMed
description Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity measurements. We show that a uniform layer was obtained by activating the oxide before immobilization. X-ray Photoelectron Spectroscopy measurements carried out on bulk oxide showed that the silicon substrate signal was fully screened after the enzyme deposition showing the absence of uncovered surface regions. The enzyme presence was detected monitoring both the C 1s and N 1s signals. Finally, enzymatic activity measurements confirmed that the glucose oxidase activity was preserved after immobilization and maintained after three months of shelf life if the sample was properly stored. The importance of using porous silicon oxide to maximize the surface area was also evidenced.
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spelling pubmed-37055232013-07-09 Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces Libertino, Sebania Aiello, Venera Scandurra, Antonino Renis, Marcella Sinatra, Fulvia Sensors (Basel) Article Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity measurements. We show that a uniform layer was obtained by activating the oxide before immobilization. X-ray Photoelectron Spectroscopy measurements carried out on bulk oxide showed that the silicon substrate signal was fully screened after the enzyme deposition showing the absence of uncovered surface regions. The enzyme presence was detected monitoring both the C 1s and N 1s signals. Finally, enzymatic activity measurements confirmed that the glucose oxidase activity was preserved after immobilization and maintained after three months of shelf life if the sample was properly stored. The importance of using porous silicon oxide to maximize the surface area was also evidenced. Molecular Diversity Preservation International (MDPI) 2008-09-15 /pmc/articles/PMC3705523/ /pubmed/27873833 http://dx.doi.org/10.3390/s8095637 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Libertino, Sebania
Aiello, Venera
Scandurra, Antonino
Renis, Marcella
Sinatra, Fulvia
Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces
title Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces
title_full Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces
title_fullStr Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces
title_full_unstemmed Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces
title_short Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces
title_sort immobilization of the enzyme glucose oxidase on both bulk and porous sio(2) surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705523/
https://www.ncbi.nlm.nih.gov/pubmed/27873833
http://dx.doi.org/10.3390/s8095637
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