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Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces
Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity meas...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705523/ https://www.ncbi.nlm.nih.gov/pubmed/27873833 http://dx.doi.org/10.3390/s8095637 |
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author | Libertino, Sebania Aiello, Venera Scandurra, Antonino Renis, Marcella Sinatra, Fulvia |
author_facet | Libertino, Sebania Aiello, Venera Scandurra, Antonino Renis, Marcella Sinatra, Fulvia |
author_sort | Libertino, Sebania |
collection | PubMed |
description | Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity measurements. We show that a uniform layer was obtained by activating the oxide before immobilization. X-ray Photoelectron Spectroscopy measurements carried out on bulk oxide showed that the silicon substrate signal was fully screened after the enzyme deposition showing the absence of uncovered surface regions. The enzyme presence was detected monitoring both the C 1s and N 1s signals. Finally, enzymatic activity measurements confirmed that the glucose oxidase activity was preserved after immobilization and maintained after three months of shelf life if the sample was properly stored. The importance of using porous silicon oxide to maximize the surface area was also evidenced. |
format | Online Article Text |
id | pubmed-3705523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-37055232013-07-09 Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces Libertino, Sebania Aiello, Venera Scandurra, Antonino Renis, Marcella Sinatra, Fulvia Sensors (Basel) Article Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity measurements. We show that a uniform layer was obtained by activating the oxide before immobilization. X-ray Photoelectron Spectroscopy measurements carried out on bulk oxide showed that the silicon substrate signal was fully screened after the enzyme deposition showing the absence of uncovered surface regions. The enzyme presence was detected monitoring both the C 1s and N 1s signals. Finally, enzymatic activity measurements confirmed that the glucose oxidase activity was preserved after immobilization and maintained after three months of shelf life if the sample was properly stored. The importance of using porous silicon oxide to maximize the surface area was also evidenced. Molecular Diversity Preservation International (MDPI) 2008-09-15 /pmc/articles/PMC3705523/ /pubmed/27873833 http://dx.doi.org/10.3390/s8095637 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Libertino, Sebania Aiello, Venera Scandurra, Antonino Renis, Marcella Sinatra, Fulvia Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces |
title | Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces |
title_full | Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces |
title_fullStr | Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces |
title_full_unstemmed | Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces |
title_short | Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO(2) Surfaces |
title_sort | immobilization of the enzyme glucose oxidase on both bulk and porous sio(2) surfaces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705523/ https://www.ncbi.nlm.nih.gov/pubmed/27873833 http://dx.doi.org/10.3390/s8095637 |
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