Cargando…

Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV) Found in Cervical Lesions of Women from North-East Brazil

The aim of this study was to examine the prevalence and genetic variability of the capsid L1 gene of rare HPV genotypes that were found in the cervical lesions of women from North-East Brazil. A total number of 263 patients were included in this study. HPV detection was performed using PCR followed...

Descripción completa

Detalles Bibliográficos
Autores principales: Gurgel, Ana Pavla Almeida Diniz, Chagas, Bárbara Simas, do Amaral, Carolina Maria Medeiros, Albuquerque, Eugênia Maria Bezerra, Serra, Ivi Gonçalves Soares Santos, Silva Neto, Jacinto da Costa, Muniz, Maria Tereza Cartaxo, de Freitas, Antonio Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705854/
https://www.ncbi.nlm.nih.gov/pubmed/23865057
http://dx.doi.org/10.1155/2013/546354
_version_ 1782476496044032000
author Gurgel, Ana Pavla Almeida Diniz
Chagas, Bárbara Simas
do Amaral, Carolina Maria Medeiros
Albuquerque, Eugênia Maria Bezerra
Serra, Ivi Gonçalves Soares Santos
Silva Neto, Jacinto da Costa
Muniz, Maria Tereza Cartaxo
de Freitas, Antonio Carlos
author_facet Gurgel, Ana Pavla Almeida Diniz
Chagas, Bárbara Simas
do Amaral, Carolina Maria Medeiros
Albuquerque, Eugênia Maria Bezerra
Serra, Ivi Gonçalves Soares Santos
Silva Neto, Jacinto da Costa
Muniz, Maria Tereza Cartaxo
de Freitas, Antonio Carlos
author_sort Gurgel, Ana Pavla Almeida Diniz
collection PubMed
description The aim of this study was to examine the prevalence and genetic variability of the capsid L1 gene of rare HPV genotypes that were found in the cervical lesions of women from North-East Brazil. A total number of 263 patients were included in this study. HPV detection was performed using PCR followed by direct sequencing of MY09/11, as well as type-specific PCR to detect the Alpha-9 species. Epitope prediction was performed to determine whether or not the genetic variants are inserted in B-cell and T-cell epitopes. The prevalence of rare HPV types in cervical lesions was found to be 9.47%. The rare HPV genotypes that were detected were HPV-53, 54, 56, 61, 62, 66, 70, and 81. The genetic variability in the L1 gene of rare HPV types involved thirty nucleotide changes, eight of which were detected for the first time in this study. Moreover, some of these variants are embedded in B-cell or T-cell epitope regions. The results of this research suggest that rare HPV types might be involved in cervical lesions and some of these variants can be found in B-cell and T-cell epitopes. Data on the prevalence and variability of rare HPV types will assist in clarifying the role of these viruses in carcinogenesis.
format Online
Article
Text
id pubmed-3705854
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-37058542013-07-17 Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV) Found in Cervical Lesions of Women from North-East Brazil Gurgel, Ana Pavla Almeida Diniz Chagas, Bárbara Simas do Amaral, Carolina Maria Medeiros Albuquerque, Eugênia Maria Bezerra Serra, Ivi Gonçalves Soares Santos Silva Neto, Jacinto da Costa Muniz, Maria Tereza Cartaxo de Freitas, Antonio Carlos Biomed Res Int Research Article The aim of this study was to examine the prevalence and genetic variability of the capsid L1 gene of rare HPV genotypes that were found in the cervical lesions of women from North-East Brazil. A total number of 263 patients were included in this study. HPV detection was performed using PCR followed by direct sequencing of MY09/11, as well as type-specific PCR to detect the Alpha-9 species. Epitope prediction was performed to determine whether or not the genetic variants are inserted in B-cell and T-cell epitopes. The prevalence of rare HPV types in cervical lesions was found to be 9.47%. The rare HPV genotypes that were detected were HPV-53, 54, 56, 61, 62, 66, 70, and 81. The genetic variability in the L1 gene of rare HPV types involved thirty nucleotide changes, eight of which were detected for the first time in this study. Moreover, some of these variants are embedded in B-cell or T-cell epitope regions. The results of this research suggest that rare HPV types might be involved in cervical lesions and some of these variants can be found in B-cell and T-cell epitopes. Data on the prevalence and variability of rare HPV types will assist in clarifying the role of these viruses in carcinogenesis. Hindawi Publishing Corporation 2013 2013-06-20 /pmc/articles/PMC3705854/ /pubmed/23865057 http://dx.doi.org/10.1155/2013/546354 Text en Copyright © 2013 Ana Pavla Almeida Diniz Gurgel et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gurgel, Ana Pavla Almeida Diniz
Chagas, Bárbara Simas
do Amaral, Carolina Maria Medeiros
Albuquerque, Eugênia Maria Bezerra
Serra, Ivi Gonçalves Soares Santos
Silva Neto, Jacinto da Costa
Muniz, Maria Tereza Cartaxo
de Freitas, Antonio Carlos
Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV) Found in Cervical Lesions of Women from North-East Brazil
title Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV) Found in Cervical Lesions of Women from North-East Brazil
title_full Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV) Found in Cervical Lesions of Women from North-East Brazil
title_fullStr Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV) Found in Cervical Lesions of Women from North-East Brazil
title_full_unstemmed Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV) Found in Cervical Lesions of Women from North-East Brazil
title_short Prevalence and Genetic Variability in Capsid L1 Gene of Rare Human Papillomaviruses (HPV) Found in Cervical Lesions of Women from North-East Brazil
title_sort prevalence and genetic variability in capsid l1 gene of rare human papillomaviruses (hpv) found in cervical lesions of women from north-east brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705854/
https://www.ncbi.nlm.nih.gov/pubmed/23865057
http://dx.doi.org/10.1155/2013/546354
work_keys_str_mv AT gurgelanapavlaalmeidadiniz prevalenceandgeneticvariabilityincapsidl1geneofrarehumanpapillomaviruseshpvfoundincervicallesionsofwomenfromnortheastbrazil
AT chagasbarbarasimas prevalenceandgeneticvariabilityincapsidl1geneofrarehumanpapillomaviruseshpvfoundincervicallesionsofwomenfromnortheastbrazil
AT doamaralcarolinamariamedeiros prevalenceandgeneticvariabilityincapsidl1geneofrarehumanpapillomaviruseshpvfoundincervicallesionsofwomenfromnortheastbrazil
AT albuquerqueeugeniamariabezerra prevalenceandgeneticvariabilityincapsidl1geneofrarehumanpapillomaviruseshpvfoundincervicallesionsofwomenfromnortheastbrazil
AT serraivigoncalvessoaressantos prevalenceandgeneticvariabilityincapsidl1geneofrarehumanpapillomaviruseshpvfoundincervicallesionsofwomenfromnortheastbrazil
AT silvanetojacintodacosta prevalenceandgeneticvariabilityincapsidl1geneofrarehumanpapillomaviruseshpvfoundincervicallesionsofwomenfromnortheastbrazil
AT munizmariaterezacartaxo prevalenceandgeneticvariabilityincapsidl1geneofrarehumanpapillomaviruseshpvfoundincervicallesionsofwomenfromnortheastbrazil
AT defreitasantoniocarlos prevalenceandgeneticvariabilityincapsidl1geneofrarehumanpapillomaviruseshpvfoundincervicallesionsofwomenfromnortheastbrazil