Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK...

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Autores principales: Piluso, Susanna, Cassell, Heather C., Gibbons, Jonathan L., Waller, Thomas E., Plant, Nick J., Miller, Aline F., Cavalli, Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2013
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705885/
https://www.ncbi.nlm.nih.gov/pubmed/23847687
http://dx.doi.org/10.1039/c3sm00131h
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author Piluso, Susanna
Cassell, Heather C.
Gibbons, Jonathan L.
Waller, Thomas E.
Plant, Nick J.
Miller, Aline F.
Cavalli, Gabriel
author_facet Piluso, Susanna
Cassell, Heather C.
Gibbons, Jonathan L.
Waller, Thomas E.
Plant, Nick J.
Miller, Aline F.
Cavalli, Gabriel
author_sort Piluso, Susanna
collection PubMed
description The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.
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spelling pubmed-37058852013-07-09 Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool Piluso, Susanna Cassell, Heather C. Gibbons, Jonathan L. Waller, Thomas E. Plant, Nick J. Miller, Aline F. Cavalli, Gabriel Soft Matter Chemistry The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties. Royal Society of Chemistry 2013-08-07 2013-03-07 /pmc/articles/PMC3705885/ /pubmed/23847687 http://dx.doi.org/10.1039/c3sm00131h Text en This journal is © The Royal Society of Chemistry 2013 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Piluso, Susanna
Cassell, Heather C.
Gibbons, Jonathan L.
Waller, Thomas E.
Plant, Nick J.
Miller, Aline F.
Cavalli, Gabriel
Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
title Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
title_full Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
title_fullStr Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
title_full_unstemmed Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
title_short Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
title_sort site-specific, covalent incorporation of tus, a dna-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase sortase a as a conjugation tool
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705885/
https://www.ncbi.nlm.nih.gov/pubmed/23847687
http://dx.doi.org/10.1039/c3sm00131h
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