Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture

We detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the c...

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Autores principales: Akita, Masumi, Tanaka, Kayoko, Matsumoto, Sachiko, Komatsu, Kumiko, Fujita, Keiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706061/
https://www.ncbi.nlm.nih.gov/pubmed/23864867
http://dx.doi.org/10.1155/2013/927403
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author Akita, Masumi
Tanaka, Kayoko
Matsumoto, Sachiko
Komatsu, Kumiko
Fujita, Keiko
author_facet Akita, Masumi
Tanaka, Kayoko
Matsumoto, Sachiko
Komatsu, Kumiko
Fujita, Keiko
author_sort Akita, Masumi
collection PubMed
description We detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the capacity to form capillary tubes. Lovastatin strongly inhibited cell migration from aortic explants and caused the degradation of the capillary tubes. The present study provides insight into the function of CD133 during angiogenesis as well as an explanation for the antiangiogenic effect of statins.
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spelling pubmed-37060612013-07-17 Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture Akita, Masumi Tanaka, Kayoko Matsumoto, Sachiko Komatsu, Kumiko Fujita, Keiko Stem Cells Int Research Article We detected the hematopoietic stem and progenitor cell marker CD133 using immunogold labeling during angiogenesis in a three-dimensional collagen gel culture. CD133-positive cells were present in capillary tubes newly formed from aortic explants in vitro. The CD133-positive cell population had the capacity to form capillary tubes. Lovastatin strongly inhibited cell migration from aortic explants and caused the degradation of the capillary tubes. The present study provides insight into the function of CD133 during angiogenesis as well as an explanation for the antiangiogenic effect of statins. Hindawi Publishing Corporation 2013 2013-06-23 /pmc/articles/PMC3706061/ /pubmed/23864867 http://dx.doi.org/10.1155/2013/927403 Text en Copyright © 2013 Masumi Akita et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Akita, Masumi
Tanaka, Kayoko
Matsumoto, Sachiko
Komatsu, Kumiko
Fujita, Keiko
Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
title Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
title_full Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
title_fullStr Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
title_full_unstemmed Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
title_short Detection of the Hematopoietic Stem and Progenitor Cell Marker CD133 during Angiogenesis in Three-Dimensional Collagen Gel Culture
title_sort detection of the hematopoietic stem and progenitor cell marker cd133 during angiogenesis in three-dimensional collagen gel culture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706061/
https://www.ncbi.nlm.nih.gov/pubmed/23864867
http://dx.doi.org/10.1155/2013/927403
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