Cargando…

Elastomeric microparticles for acoustic mediated bioseparations

BACKGROUND: Acoustophoresis has been utilized successfully in applications including cell trapping, focusing, and purification. One current limitation of acoustophoresis for cell sorting is the reliance on the inherent physical properties of cells (e.g., compressibility, density) instead of selectin...

Descripción completa

Detalles Bibliográficos
Autores principales: Johnson, Leah M, Gao, Lu, Shields IV, C Wyatt, Smith, Margret, Efimenko, Kirill, Cushing, Kevin, Genzer, Jan, López, Gabriel P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706277/
https://www.ncbi.nlm.nih.gov/pubmed/23809852
http://dx.doi.org/10.1186/1477-3155-11-22
_version_ 1782476529449566208
author Johnson, Leah M
Gao, Lu
Shields IV, C Wyatt
Smith, Margret
Efimenko, Kirill
Cushing, Kevin
Genzer, Jan
López, Gabriel P
author_facet Johnson, Leah M
Gao, Lu
Shields IV, C Wyatt
Smith, Margret
Efimenko, Kirill
Cushing, Kevin
Genzer, Jan
López, Gabriel P
author_sort Johnson, Leah M
collection PubMed
description BACKGROUND: Acoustophoresis has been utilized successfully in applications including cell trapping, focusing, and purification. One current limitation of acoustophoresis for cell sorting is the reliance on the inherent physical properties of cells (e.g., compressibility, density) instead of selecting cells based upon biologically relevant surface-presenting antigens. Introducing an acoustophoretic cell sorting approach that allows biochemical specificity may overcome this limitation, thus advancing the value of acoustophoresis approaches for both the basic research and clinical fields. RESULTS: The results presented herein demonstrate the ability for negative acoustic contrast particles (NACPs) to specifically capture and transport positive acoustic contrast particles (PACPs) to the antinode of an ultrasound standing wave. Emulsification and post curing of pre-polymers, either polydimethylsiloxane (PDMS) or polyvinylmethylsiloxane (PVMS), within aqueous surfactant solution results in the formation of stable NACPs that focus onto pressure antinodes. We used either photochemical reactions with biotin-tetrafluorophenyl azide (biotin-TFPA) or end-functionalization of Pluronic F108 surfactant to biofunctionalize NACPs. These biotinylated NACPs bind specifically to streptavidin polystyrene microparticles (as cell surrogates) and transport them to the pressure antinode within an acoustofluidic chip. CONCLUSION: To the best of our knowledge, this is the first demonstration of using NACPs as carriers for transport of PACPs in an ultrasound standing wave. By using different silicones (i.e., PDMS, PVMS) and curing chemistries, we demonstrate versatility of silicone materials for NACPs and advance the understanding of useful approaches for preparing NACPs. This bioseparation scheme holds potential for applications requiring rapid, continuous separations such as sorting and analysis of cells and biomolecules.
format Online
Article
Text
id pubmed-3706277
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-37062772013-07-15 Elastomeric microparticles for acoustic mediated bioseparations Johnson, Leah M Gao, Lu Shields IV, C Wyatt Smith, Margret Efimenko, Kirill Cushing, Kevin Genzer, Jan López, Gabriel P J Nanobiotechnology Methodology BACKGROUND: Acoustophoresis has been utilized successfully in applications including cell trapping, focusing, and purification. One current limitation of acoustophoresis for cell sorting is the reliance on the inherent physical properties of cells (e.g., compressibility, density) instead of selecting cells based upon biologically relevant surface-presenting antigens. Introducing an acoustophoretic cell sorting approach that allows biochemical specificity may overcome this limitation, thus advancing the value of acoustophoresis approaches for both the basic research and clinical fields. RESULTS: The results presented herein demonstrate the ability for negative acoustic contrast particles (NACPs) to specifically capture and transport positive acoustic contrast particles (PACPs) to the antinode of an ultrasound standing wave. Emulsification and post curing of pre-polymers, either polydimethylsiloxane (PDMS) or polyvinylmethylsiloxane (PVMS), within aqueous surfactant solution results in the formation of stable NACPs that focus onto pressure antinodes. We used either photochemical reactions with biotin-tetrafluorophenyl azide (biotin-TFPA) or end-functionalization of Pluronic F108 surfactant to biofunctionalize NACPs. These biotinylated NACPs bind specifically to streptavidin polystyrene microparticles (as cell surrogates) and transport them to the pressure antinode within an acoustofluidic chip. CONCLUSION: To the best of our knowledge, this is the first demonstration of using NACPs as carriers for transport of PACPs in an ultrasound standing wave. By using different silicones (i.e., PDMS, PVMS) and curing chemistries, we demonstrate versatility of silicone materials for NACPs and advance the understanding of useful approaches for preparing NACPs. This bioseparation scheme holds potential for applications requiring rapid, continuous separations such as sorting and analysis of cells and biomolecules. BioMed Central 2013-06-28 /pmc/articles/PMC3706277/ /pubmed/23809852 http://dx.doi.org/10.1186/1477-3155-11-22 Text en Copyright © 2013 Johnson et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Johnson, Leah M
Gao, Lu
Shields IV, C Wyatt
Smith, Margret
Efimenko, Kirill
Cushing, Kevin
Genzer, Jan
López, Gabriel P
Elastomeric microparticles for acoustic mediated bioseparations
title Elastomeric microparticles for acoustic mediated bioseparations
title_full Elastomeric microparticles for acoustic mediated bioseparations
title_fullStr Elastomeric microparticles for acoustic mediated bioseparations
title_full_unstemmed Elastomeric microparticles for acoustic mediated bioseparations
title_short Elastomeric microparticles for acoustic mediated bioseparations
title_sort elastomeric microparticles for acoustic mediated bioseparations
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706277/
https://www.ncbi.nlm.nih.gov/pubmed/23809852
http://dx.doi.org/10.1186/1477-3155-11-22
work_keys_str_mv AT johnsonleahm elastomericmicroparticlesforacousticmediatedbioseparations
AT gaolu elastomericmicroparticlesforacousticmediatedbioseparations
AT shieldsivcwyatt elastomericmicroparticlesforacousticmediatedbioseparations
AT smithmargret elastomericmicroparticlesforacousticmediatedbioseparations
AT efimenkokirill elastomericmicroparticlesforacousticmediatedbioseparations
AT cushingkevin elastomericmicroparticlesforacousticmediatedbioseparations
AT genzerjan elastomericmicroparticlesforacousticmediatedbioseparations
AT lopezgabrielp elastomericmicroparticlesforacousticmediatedbioseparations