Cargando…
Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous zebrafish genes. Here we demonstrate that RGN-in...
Autores principales: | Hwang, Woong Y., Fu, Yanfang, Reyon, Deepak, Maeder, Morgan L., Kaini, Prakriti, Sander, Jeffry D., Joung, J. Keith, Peterson, Randall T., Yeh, Jing-Ruey Joanna |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706373/ https://www.ncbi.nlm.nih.gov/pubmed/23874735 http://dx.doi.org/10.1371/journal.pone.0068708 |
Ejemplares similares
-
Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs
por: Cade, Lindsay, et al.
Publicado: (2012) -
Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)
por: Foley, Jonathan E., et al.
Publicado: (2009) -
High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
por: Fu, Yanfang, et al.
Publicado: (2013) -
Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
por: Hwang, Woong Y., et al.
Publicado: (2013) -
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
por: Fu, Yanfang, et al.
Publicado: (2014)