Improved coverage and accuracy with strand-conserving sequence enrichment

Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven targ...

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Detalles Bibliográficos
Autores principales: Guettouche, Toumy, Zuchner, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research Highlight
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706846/
https://www.ncbi.nlm.nih.gov/pubmed/23731654
http://dx.doi.org/10.1186/gm450
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author Guettouche, Toumy
Zuchner, Stephan
author_facet Guettouche, Toumy
Zuchner, Stephan
author_sort Guettouche, Toumy
collection PubMed
description Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven target coverage. A target-enrichment approach using complementary long padlock probes described in a recent article significantly improves on previous methods in most of these areas. See related Research: http://genomemedicine.com/content/5/5/50
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spelling pubmed-37068462013-07-15 Improved coverage and accuracy with strand-conserving sequence enrichment Guettouche, Toumy Zuchner, Stephan Genome Med Research Highlight Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven target coverage. A target-enrichment approach using complementary long padlock probes described in a recent article significantly improves on previous methods in most of these areas. See related Research: http://genomemedicine.com/content/5/5/50 BioMed Central 2013-05-29 /pmc/articles/PMC3706846/ /pubmed/23731654 http://dx.doi.org/10.1186/gm450 Text en Copyright © 2013 BioMed Central Ltd
spellingShingle Research Highlight
Guettouche, Toumy
Zuchner, Stephan
Improved coverage and accuracy with strand-conserving sequence enrichment
title Improved coverage and accuracy with strand-conserving sequence enrichment
title_full Improved coverage and accuracy with strand-conserving sequence enrichment
title_fullStr Improved coverage and accuracy with strand-conserving sequence enrichment
title_full_unstemmed Improved coverage and accuracy with strand-conserving sequence enrichment
title_short Improved coverage and accuracy with strand-conserving sequence enrichment
title_sort improved coverage and accuracy with strand-conserving sequence enrichment
topic Research Highlight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706846/
https://www.ncbi.nlm.nih.gov/pubmed/23731654
http://dx.doi.org/10.1186/gm450
work_keys_str_mv AT guettouchetoumy improvedcoverageandaccuracywithstrandconservingsequenceenrichment
AT zuchnerstephan improvedcoverageandaccuracywithstrandconservingsequenceenrichment

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