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Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae

Spectinomycin remains a useful reserve option for therapy of gonorrhea. The emergence of multidrug-resistant Neisseria gonorrhoeae strains with decreased susceptibility to cefixime and to ceftriaxone makes it the only medicine still effective for treatment of gonorrhea infection in analogous cases....

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Autores principales: Ilina, Elena N., Malakhova, Maya V., Bodoev, Ivan N., Oparina, Nina Y., Filimonova, Alla V., Govorun, Vadim M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706878/
https://www.ncbi.nlm.nih.gov/pubmed/23847609
http://dx.doi.org/10.3389/fmicb.2013.00186
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author Ilina, Elena N.
Malakhova, Maya V.
Bodoev, Ivan N.
Oparina, Nina Y.
Filimonova, Alla V.
Govorun, Vadim M.
author_facet Ilina, Elena N.
Malakhova, Maya V.
Bodoev, Ivan N.
Oparina, Nina Y.
Filimonova, Alla V.
Govorun, Vadim M.
author_sort Ilina, Elena N.
collection PubMed
description Spectinomycin remains a useful reserve option for therapy of gonorrhea. The emergence of multidrug-resistant Neisseria gonorrhoeae strains with decreased susceptibility to cefixime and to ceftriaxone makes it the only medicine still effective for treatment of gonorrhea infection in analogous cases. However, adoption of spectinomycin as a routinely used drug of choice was soon followed by reports of spectinomycin resistance. The main molecular mechanism of spectinomycin resistance in N. gonorrhoeae was C1192T substitution in 16S rRNA genes. Here we reported a Thr-24→Pro mutation in ribosomal protein S5 (RPS5) found in spectinomycin resistant clinical N. gonorrhoeae strain, which carried no changes in 16S rRNA. In a series of experiments, the transfer of rpsE gene allele encoding the mutant RPS5 to the recipient N. gonorrhoeae strains was analyzed. The relatively high rate of transformation [ca. 10(−5) colony-forming units (CFUs)] indicates the possibility of spread of spectinonycin resistance within gonococcal population due to the horizontal gene transfer (HGT).
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spelling pubmed-37068782013-07-11 Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae Ilina, Elena N. Malakhova, Maya V. Bodoev, Ivan N. Oparina, Nina Y. Filimonova, Alla V. Govorun, Vadim M. Front Microbiol Microbiology Spectinomycin remains a useful reserve option for therapy of gonorrhea. The emergence of multidrug-resistant Neisseria gonorrhoeae strains with decreased susceptibility to cefixime and to ceftriaxone makes it the only medicine still effective for treatment of gonorrhea infection in analogous cases. However, adoption of spectinomycin as a routinely used drug of choice was soon followed by reports of spectinomycin resistance. The main molecular mechanism of spectinomycin resistance in N. gonorrhoeae was C1192T substitution in 16S rRNA genes. Here we reported a Thr-24→Pro mutation in ribosomal protein S5 (RPS5) found in spectinomycin resistant clinical N. gonorrhoeae strain, which carried no changes in 16S rRNA. In a series of experiments, the transfer of rpsE gene allele encoding the mutant RPS5 to the recipient N. gonorrhoeae strains was analyzed. The relatively high rate of transformation [ca. 10(−5) colony-forming units (CFUs)] indicates the possibility of spread of spectinonycin resistance within gonococcal population due to the horizontal gene transfer (HGT). Frontiers Media S.A. 2013-07-10 /pmc/articles/PMC3706878/ /pubmed/23847609 http://dx.doi.org/10.3389/fmicb.2013.00186 Text en Copyright © 2013 Ilina, Malakhova, Bodoev, Oparina, Filimonova and Govorun. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Microbiology
Ilina, Elena N.
Malakhova, Maya V.
Bodoev, Ivan N.
Oparina, Nina Y.
Filimonova, Alla V.
Govorun, Vadim M.
Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae
title Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae
title_full Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae
title_fullStr Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae
title_full_unstemmed Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae
title_short Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae
title_sort mutation in ribosomal protein s5 leads to spectinomycin resistance in neisseria gonorrhoeae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706878/
https://www.ncbi.nlm.nih.gov/pubmed/23847609
http://dx.doi.org/10.3389/fmicb.2013.00186
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