Cargando…

Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury

INTRODUCTION: Nervous system injuries comprise a diverse group of disorders that include traumatic brain injury (TBI). The potential of mesenchymal stem cells (MSCs) to differentiate into neural cell types has aroused hope for the possible development of autologous therapies for central nervous syst...

Descripción completa

Detalles Bibliográficos
Autores principales: Nichols, Joan E, Niles, Jean A, DeWitt, Douglas, Prough, Donald, Parsley, Margaret, Vega, Stephanie, Cantu, Andrea, Lee, Eric, Cortiella, Joaquin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707064/
https://www.ncbi.nlm.nih.gov/pubmed/23290300
http://dx.doi.org/10.1186/scrt151
_version_ 1782276463971532800
author Nichols, Joan E
Niles, Jean A
DeWitt, Douglas
Prough, Donald
Parsley, Margaret
Vega, Stephanie
Cantu, Andrea
Lee, Eric
Cortiella, Joaquin
author_facet Nichols, Joan E
Niles, Jean A
DeWitt, Douglas
Prough, Donald
Parsley, Margaret
Vega, Stephanie
Cantu, Andrea
Lee, Eric
Cortiella, Joaquin
author_sort Nichols, Joan E
collection PubMed
description INTRODUCTION: Nervous system injuries comprise a diverse group of disorders that include traumatic brain injury (TBI). The potential of mesenchymal stem cells (MSCs) to differentiate into neural cell types has aroused hope for the possible development of autologous therapies for central nervous system injury. METHODS: In this study we isolated and characterized a human peripheral blood derived (HPBD) MSC population which we examined for neural lineage potential and ability to migrate in vitro and in vivo. HPBD CD133+, ATP-binding cassette sub-family G member 2 (ABCG2)+, C-X-C chemokine receptor type 4 (CXCR4)+ MSCs were differentiated after priming with β-mercaptoethanol (β-ME) combined with trans-retinoic acid (RA) and culture in neural basal media containing basic fibroblast growth factor (FGF2) and epidermal growth factor (EGF) or co-culture with neuronal cell lines. Differentiation efficiencies in vitro were determined using flow cytometry or fluorescent microscopy of cytospins made of FACS sorted positive cells after staining for markers of immature or mature neuronal lineages. RA-primed CD133+ABCG2+CXCR4+ human MSCs were transplanted into the lateral ventricle of male Sprague-Dawley rats, 24 hours after sham or traumatic brain injury (TBI). All animals were evaluated for spatial memory performance using the Morris Water Maze (MWM) Test. Histological examination of sham or TBI brains was done to evaluate MSC survival, migration and differentiation into neural lineages. We also examined induction of apoptosis at the injury site and production of MSC neuroprotective factors. RESULTS: CD133+ABCG2+CXCR4+ MSCs consistently expressed markers of neural lineage induction and were positive for nestin, microtubule associated protein-1β (MAP-1β), tyrosine hydroxylase (TH), neuron specific nuclear protein (NEUN) or type III beta-tubulin (Tuj1). Animals in the primed MSC treatment group exhibited MWM latency results similar to the uninjured (sham) group with both groups showing improvements in latency. Histological examination of brains of these animals showed that in uninjured animals the majority of MSCs were found in the lateral ventricle, the site of transplantation, while in TBI rats MSCs were consistently found in locations near the injury site. We found that levels of apoptosis were less in MSC treated rats and that MSCs could be shown to produce neurotropic factors as early as 2 days following transplantation of cells. In TBI rats, at 1 and 3 months post transplantation cells were generated which expressed markers of neural lineages including immature as well as mature neurons. CONCLUSIONS: These results suggest that PBD CD133+ABCG2+CXCR4+ MSCs have the potential for development as an autologous treatment for TBI and neurodegenerative disorders and that MSC derived cell products produced immediately after transplantation may aid in reducing the immediate cognitive defects of TBI.
format Online
Article
Text
id pubmed-3707064
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-37070642013-07-10 Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury Nichols, Joan E Niles, Jean A DeWitt, Douglas Prough, Donald Parsley, Margaret Vega, Stephanie Cantu, Andrea Lee, Eric Cortiella, Joaquin Stem Cell Res Ther Research INTRODUCTION: Nervous system injuries comprise a diverse group of disorders that include traumatic brain injury (TBI). The potential of mesenchymal stem cells (MSCs) to differentiate into neural cell types has aroused hope for the possible development of autologous therapies for central nervous system injury. METHODS: In this study we isolated and characterized a human peripheral blood derived (HPBD) MSC population which we examined for neural lineage potential and ability to migrate in vitro and in vivo. HPBD CD133+, ATP-binding cassette sub-family G member 2 (ABCG2)+, C-X-C chemokine receptor type 4 (CXCR4)+ MSCs were differentiated after priming with β-mercaptoethanol (β-ME) combined with trans-retinoic acid (RA) and culture in neural basal media containing basic fibroblast growth factor (FGF2) and epidermal growth factor (EGF) or co-culture with neuronal cell lines. Differentiation efficiencies in vitro were determined using flow cytometry or fluorescent microscopy of cytospins made of FACS sorted positive cells after staining for markers of immature or mature neuronal lineages. RA-primed CD133+ABCG2+CXCR4+ human MSCs were transplanted into the lateral ventricle of male Sprague-Dawley rats, 24 hours after sham or traumatic brain injury (TBI). All animals were evaluated for spatial memory performance using the Morris Water Maze (MWM) Test. Histological examination of sham or TBI brains was done to evaluate MSC survival, migration and differentiation into neural lineages. We also examined induction of apoptosis at the injury site and production of MSC neuroprotective factors. RESULTS: CD133+ABCG2+CXCR4+ MSCs consistently expressed markers of neural lineage induction and were positive for nestin, microtubule associated protein-1β (MAP-1β), tyrosine hydroxylase (TH), neuron specific nuclear protein (NEUN) or type III beta-tubulin (Tuj1). Animals in the primed MSC treatment group exhibited MWM latency results similar to the uninjured (sham) group with both groups showing improvements in latency. Histological examination of brains of these animals showed that in uninjured animals the majority of MSCs were found in the lateral ventricle, the site of transplantation, while in TBI rats MSCs were consistently found in locations near the injury site. We found that levels of apoptosis were less in MSC treated rats and that MSCs could be shown to produce neurotropic factors as early as 2 days following transplantation of cells. In TBI rats, at 1 and 3 months post transplantation cells were generated which expressed markers of neural lineages including immature as well as mature neurons. CONCLUSIONS: These results suggest that PBD CD133+ABCG2+CXCR4+ MSCs have the potential for development as an autologous treatment for TBI and neurodegenerative disorders and that MSC derived cell products produced immediately after transplantation may aid in reducing the immediate cognitive defects of TBI. BioMed Central 2013-01-06 /pmc/articles/PMC3707064/ /pubmed/23290300 http://dx.doi.org/10.1186/scrt151 Text en Copyright © 2013 Nichols et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Nichols, Joan E
Niles, Jean A
DeWitt, Douglas
Prough, Donald
Parsley, Margaret
Vega, Stephanie
Cantu, Andrea
Lee, Eric
Cortiella, Joaquin
Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury
title Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury
title_full Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury
title_fullStr Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury
title_full_unstemmed Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury
title_short Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury
title_sort neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived cd133+ abcg2+cxcr4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707064/
https://www.ncbi.nlm.nih.gov/pubmed/23290300
http://dx.doi.org/10.1186/scrt151
work_keys_str_mv AT nicholsjoane neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury
AT nilesjeana neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury
AT dewittdouglas neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury
AT proughdonald neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury
AT parsleymargaret neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury
AT vegastephanie neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury
AT cantuandrea neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury
AT leeeric neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury
AT cortiellajoaquin neurogenicandneuroprotectivepotentialofanovelsubpopulationofperipheralbloodderivedcd133abcg2cxcr4mesenchymalstemcellsdevelopmentofautologouscellbasedtherapeuticsfortraumaticbraininjury