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Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium

Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey's fibers of the periodontal ligament (PDL). However, little is known about the regulator...

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Autores principales: Rakian, Audrey, Yang, Wu-Chen, Gluhak-Heinrich, Jelica, Cui, Yong, Harris, Marie A, Villarreal, Demitri, Feng, Jerry Q, MacDougall, Mary, Harris, Stephen E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707077/
https://www.ncbi.nlm.nih.gov/pubmed/23807640
http://dx.doi.org/10.1038/ijos.2013.41
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author Rakian, Audrey
Yang, Wu-Chen
Gluhak-Heinrich, Jelica
Cui, Yong
Harris, Marie A
Villarreal, Demitri
Feng, Jerry Q
MacDougall, Mary
Harris, Stephen E
author_facet Rakian, Audrey
Yang, Wu-Chen
Gluhak-Heinrich, Jelica
Cui, Yong
Harris, Marie A
Villarreal, Demitri
Feng, Jerry Q
MacDougall, Mary
Harris, Stephen E
author_sort Rakian, Audrey
collection PubMed
description Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey's fibers of the periodontal ligament (PDL). However, little is known about the regulatory morphogens that control differentiation and function of these progenitor cells, as well as the progenitor cells involved in crown and root formation. We investigated the role of bone morphogenetic protein-2 (Bmp2) in these processes by the conditional removal of the Bmp2 gene using the Sp7-Cre-EGFP mouse model. Sp7-Cre-EGFP first becomes active at E18 in the first molar, with robust Cre activity at postnatal day 0 (P0), followed by Cre activity in the second molar, which occurs after P0. There is robust Cre activity in the periodontium and third molars by 2 weeks of age. When the Bmp2 gene is removed from Sp7(+) (Osterix(+)) cells, major defects are noted in root, cellular cementum and periodontium formation. First, there are major cell autonomous defects in root-odontoblast terminal differentiation. Second, there are major alterations in formation of the PDLs and cellular cementum, correlated with decreased nuclear factor IC (Nfic), periostin and α-SMA(+) cells. Third, there is a failure to produce vascular endothelial growth factor A (VEGF-A) in the periodontium and the pulp leading to decreased formation of the microvascular and associated candidate stem cells in the Bmp2-cKO(Sp7-Cre-EGFP). Fourth, ameloblast function and enamel formation are indirectly altered in the Bmp2-cKO(Sp7-Cre-EGFP). These data demonstrate that the Bmp2 gene has complex roles in postnatal tooth development and periodontium formation.
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spelling pubmed-37070772013-07-10 Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium Rakian, Audrey Yang, Wu-Chen Gluhak-Heinrich, Jelica Cui, Yong Harris, Marie A Villarreal, Demitri Feng, Jerry Q MacDougall, Mary Harris, Stephen E Int J Oral Sci Original Article Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey's fibers of the periodontal ligament (PDL). However, little is known about the regulatory morphogens that control differentiation and function of these progenitor cells, as well as the progenitor cells involved in crown and root formation. We investigated the role of bone morphogenetic protein-2 (Bmp2) in these processes by the conditional removal of the Bmp2 gene using the Sp7-Cre-EGFP mouse model. Sp7-Cre-EGFP first becomes active at E18 in the first molar, with robust Cre activity at postnatal day 0 (P0), followed by Cre activity in the second molar, which occurs after P0. There is robust Cre activity in the periodontium and third molars by 2 weeks of age. When the Bmp2 gene is removed from Sp7(+) (Osterix(+)) cells, major defects are noted in root, cellular cementum and periodontium formation. First, there are major cell autonomous defects in root-odontoblast terminal differentiation. Second, there are major alterations in formation of the PDLs and cellular cementum, correlated with decreased nuclear factor IC (Nfic), periostin and α-SMA(+) cells. Third, there is a failure to produce vascular endothelial growth factor A (VEGF-A) in the periodontium and the pulp leading to decreased formation of the microvascular and associated candidate stem cells in the Bmp2-cKO(Sp7-Cre-EGFP). Fourth, ameloblast function and enamel formation are indirectly altered in the Bmp2-cKO(Sp7-Cre-EGFP). These data demonstrate that the Bmp2 gene has complex roles in postnatal tooth development and periodontium formation. Nature Publishing Group 2013-06 2013-06-28 /pmc/articles/PMC3707077/ /pubmed/23807640 http://dx.doi.org/10.1038/ijos.2013.41 Text en Copyright © 2013 West China School of Stomatology http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Rakian, Audrey
Yang, Wu-Chen
Gluhak-Heinrich, Jelica
Cui, Yong
Harris, Marie A
Villarreal, Demitri
Feng, Jerry Q
MacDougall, Mary
Harris, Stephen E
Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium
title Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium
title_full Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium
title_fullStr Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium
title_full_unstemmed Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium
title_short Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium
title_sort bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707077/
https://www.ncbi.nlm.nih.gov/pubmed/23807640
http://dx.doi.org/10.1038/ijos.2013.41
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