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Intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity in neonatal rats
BACKGROUND: We previously showed that intra-amniotic lipopolysaccharide (LPS) amplifies alveolar hypoplasia induced by postnatal hyperoxia. We determined whether the priming effect of intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity (AHR). METHODS: LPS or normal saline was injec...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707085/ https://www.ncbi.nlm.nih.gov/pubmed/23563192 http://dx.doi.org/10.1038/pr.2013.58 |
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author | Choi, Chang Won Kim, Beyong Il Mason, Stanley N. Potts-Kant, Erin N. Brahmajothi, Mulugu V. Auten, Richard L. |
author_facet | Choi, Chang Won Kim, Beyong Il Mason, Stanley N. Potts-Kant, Erin N. Brahmajothi, Mulugu V. Auten, Richard L. |
author_sort | Choi, Chang Won |
collection | PubMed |
description | BACKGROUND: We previously showed that intra-amniotic lipopolysaccharide (LPS) amplifies alveolar hypoplasia induced by postnatal hyperoxia. We determined whether the priming effect of intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity (AHR). METHODS: LPS or normal saline was injected into the amniotic cavities of pregnant rats at the 20th day of gestation. After birth, rat pups were exposed to 60% O(2) or air for 14 d. On postnatal day 14, rat pups underwent forced oscillometry, which included a challenge with nebulized methacholine, and the lungs were harvested for morphological studies. RESULTS: Hyperoxia significantly increased airway reactivity and decreased compliance. Intra-amniotic LPS further increased hyperoxia-induced AHR but did not further impair respiratory system compliance. Hyperoxia-induced changes in lung parenchymal and small airway morphology were not further altered by intra-amniotic LPS. However, combined exposure to intra-amniotic LPS and hyperoxia increased the proportion of degranulating mast cells in the hilar airways. CONCLUSION: Intra-amniotic LPS amplified postnatal hyperoxia-induced AHR. This was associated with increased airway mast cell degranulation, which has previously been linked with hyperoxia-induced AHR. There were no morphologic changes of parenchyma or airways that would account for the LPS augmentation of hyperoxia-induced AHR. |
format | Online Article Text |
id | pubmed-3707085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-37070852013-07-10 Intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity in neonatal rats Choi, Chang Won Kim, Beyong Il Mason, Stanley N. Potts-Kant, Erin N. Brahmajothi, Mulugu V. Auten, Richard L. Pediatr Res Basic Science Investigation BACKGROUND: We previously showed that intra-amniotic lipopolysaccharide (LPS) amplifies alveolar hypoplasia induced by postnatal hyperoxia. We determined whether the priming effect of intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity (AHR). METHODS: LPS or normal saline was injected into the amniotic cavities of pregnant rats at the 20th day of gestation. After birth, rat pups were exposed to 60% O(2) or air for 14 d. On postnatal day 14, rat pups underwent forced oscillometry, which included a challenge with nebulized methacholine, and the lungs were harvested for morphological studies. RESULTS: Hyperoxia significantly increased airway reactivity and decreased compliance. Intra-amniotic LPS further increased hyperoxia-induced AHR but did not further impair respiratory system compliance. Hyperoxia-induced changes in lung parenchymal and small airway morphology were not further altered by intra-amniotic LPS. However, combined exposure to intra-amniotic LPS and hyperoxia increased the proportion of degranulating mast cells in the hilar airways. CONCLUSION: Intra-amniotic LPS amplified postnatal hyperoxia-induced AHR. This was associated with increased airway mast cell degranulation, which has previously been linked with hyperoxia-induced AHR. There were no morphologic changes of parenchyma or airways that would account for the LPS augmentation of hyperoxia-induced AHR. Nature Publishing Group 2013-07 2013-05-08 /pmc/articles/PMC3707085/ /pubmed/23563192 http://dx.doi.org/10.1038/pr.2013.58 Text en Copyright © 2013 International Pediatric Research Foundation, Inc. http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Basic Science Investigation Choi, Chang Won Kim, Beyong Il Mason, Stanley N. Potts-Kant, Erin N. Brahmajothi, Mulugu V. Auten, Richard L. Intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity in neonatal rats |
title | Intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity in neonatal rats |
title_full | Intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity in neonatal rats |
title_fullStr | Intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity in neonatal rats |
title_full_unstemmed | Intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity in neonatal rats |
title_short | Intra-amniotic LPS amplifies hyperoxia-induced airway hyperreactivity in neonatal rats |
title_sort | intra-amniotic lps amplifies hyperoxia-induced airway hyperreactivity in neonatal rats |
topic | Basic Science Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707085/ https://www.ncbi.nlm.nih.gov/pubmed/23563192 http://dx.doi.org/10.1038/pr.2013.58 |
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