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Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents

Interesting biological activities have been found for numerous marine compounds. In fact, screening of phylogenetically diverse marine microorganisms from extreme environments revealed to be a rational approach for the discovery of novel molecules with relevant bioactivities for industries such as p...

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Autores principales: Martins, Ana, Tenreiro, Tania, Andrade, Gonçalo, Gadanho, Mário, Chaves, Sandra, Abrantes, Marta, Calado, Patrícia, Tenreiro, Rogério, Vieira, Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707158/
https://www.ncbi.nlm.nih.gov/pubmed/23665957
http://dx.doi.org/10.3390/md11051506
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author Martins, Ana
Tenreiro, Tania
Andrade, Gonçalo
Gadanho, Mário
Chaves, Sandra
Abrantes, Marta
Calado, Patrícia
Tenreiro, Rogério
Vieira, Helena
author_facet Martins, Ana
Tenreiro, Tania
Andrade, Gonçalo
Gadanho, Mário
Chaves, Sandra
Abrantes, Marta
Calado, Patrícia
Tenreiro, Rogério
Vieira, Helena
author_sort Martins, Ana
collection PubMed
description Interesting biological activities have been found for numerous marine compounds. In fact, screening of phylogenetically diverse marine microorganisms from extreme environments revealed to be a rational approach for the discovery of novel molecules with relevant bioactivities for industries such as pharmaceutical and cosmeceutical. Nevertheless, marine sources deliverables are still far from the expectations and new extreme sources of microbes should be explored. In this work, a marine prokaryotic collection from four Mid-Atlantic Ridge (MAR) deep sea hydrothermal vents near the Azores Islands, Portugal, was created, characterized and tested for its photoprotective capacity. Within 246 isolates, a polyphasic approach, using chemotaxonomic and molecular typing methods, identified 23-related clusters of phenetically similar isolates with high indexes of diversity. Interestingly, 16S rRNA gene sequencing suggested the presence of 43% new prokaryotic species. A sub-set of 139 isolates of the prokaryotic collection was selected for biotechnological exploitation with 484 bacterial extracts prepared in a sustainable upscalling manner. 22% of the extracts showed an industrially relevant photoprotective activity, with two extracts, belonging to new strains of the species Shewanella algae and Vibrio fluvialis, uniquely showing UV-A, UV-B and UV-C protective capacity. This clearly demonstrates the high potential of the bacteria MAR vents collection in natural product synthesis with market applications.
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spelling pubmed-37071582013-07-10 Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents Martins, Ana Tenreiro, Tania Andrade, Gonçalo Gadanho, Mário Chaves, Sandra Abrantes, Marta Calado, Patrícia Tenreiro, Rogério Vieira, Helena Mar Drugs Article Interesting biological activities have been found for numerous marine compounds. In fact, screening of phylogenetically diverse marine microorganisms from extreme environments revealed to be a rational approach for the discovery of novel molecules with relevant bioactivities for industries such as pharmaceutical and cosmeceutical. Nevertheless, marine sources deliverables are still far from the expectations and new extreme sources of microbes should be explored. In this work, a marine prokaryotic collection from four Mid-Atlantic Ridge (MAR) deep sea hydrothermal vents near the Azores Islands, Portugal, was created, characterized and tested for its photoprotective capacity. Within 246 isolates, a polyphasic approach, using chemotaxonomic and molecular typing methods, identified 23-related clusters of phenetically similar isolates with high indexes of diversity. Interestingly, 16S rRNA gene sequencing suggested the presence of 43% new prokaryotic species. A sub-set of 139 isolates of the prokaryotic collection was selected for biotechnological exploitation with 484 bacterial extracts prepared in a sustainable upscalling manner. 22% of the extracts showed an industrially relevant photoprotective activity, with two extracts, belonging to new strains of the species Shewanella algae and Vibrio fluvialis, uniquely showing UV-A, UV-B and UV-C protective capacity. This clearly demonstrates the high potential of the bacteria MAR vents collection in natural product synthesis with market applications. MDPI 2013-05-10 /pmc/articles/PMC3707158/ /pubmed/23665957 http://dx.doi.org/10.3390/md11051506 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Martins, Ana
Tenreiro, Tania
Andrade, Gonçalo
Gadanho, Mário
Chaves, Sandra
Abrantes, Marta
Calado, Patrícia
Tenreiro, Rogério
Vieira, Helena
Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents
title Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents
title_full Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents
title_fullStr Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents
title_full_unstemmed Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents
title_short Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents
title_sort photoprotective bioactivity present in a unique marine bacteria collection from portuguese deep sea hydrothermal vents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707158/
https://www.ncbi.nlm.nih.gov/pubmed/23665957
http://dx.doi.org/10.3390/md11051506
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