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Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy

Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity, fluorophore concentration, and photobleaching compared t...

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Detalles Bibliográficos
Autores principales: Liu, Chao, Wang, Xinwei, Zhou, Yan, Liu, Yuliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707217/
https://www.ncbi.nlm.nih.gov/pubmed/23864834
http://dx.doi.org/10.1155/2013/801901
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author Liu, Chao
Wang, Xinwei
Zhou, Yan
Liu, Yuliang
author_facet Liu, Chao
Wang, Xinwei
Zhou, Yan
Liu, Yuliang
author_sort Liu, Chao
collection PubMed
description Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity, fluorophore concentration, and photobleaching compared to fluorescence intensity imaging techniques. Time-gated fluorescence lifetime imaging microscopy (FLIM) can provide high resolution and high imaging frame during mature FLIM methods. An abstract time-gated FLIM model was given, and important temporal parameters are shown as well. Aiming at different applications of steady and transient fluorescence processes, two different operation modes, timing and lifetime computing algorithm are designed. High resolution and high frame can be achieved by one-excitation one-sampling mode and least square algorithm for steady imaging applications. Correspondingly, one-excitation two-sampling mode and rapid lifetime determination algorithm contribute to transient fluorescence situations.
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spelling pubmed-37072172013-07-17 Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy Liu, Chao Wang, Xinwei Zhou, Yan Liu, Yuliang ScientificWorldJournal Research Article Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity, fluorophore concentration, and photobleaching compared to fluorescence intensity imaging techniques. Time-gated fluorescence lifetime imaging microscopy (FLIM) can provide high resolution and high imaging frame during mature FLIM methods. An abstract time-gated FLIM model was given, and important temporal parameters are shown as well. Aiming at different applications of steady and transient fluorescence processes, two different operation modes, timing and lifetime computing algorithm are designed. High resolution and high frame can be achieved by one-excitation one-sampling mode and least square algorithm for steady imaging applications. Correspondingly, one-excitation two-sampling mode and rapid lifetime determination algorithm contribute to transient fluorescence situations. Hindawi Publishing Corporation 2013-06-24 /pmc/articles/PMC3707217/ /pubmed/23864834 http://dx.doi.org/10.1155/2013/801901 Text en Copyright © 2013 Chao Liu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Chao
Wang, Xinwei
Zhou, Yan
Liu, Yuliang
Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
title Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
title_full Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
title_fullStr Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
title_full_unstemmed Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
title_short Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
title_sort timing and operating mode design for time-gated fluorescence lifetime imaging microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707217/
https://www.ncbi.nlm.nih.gov/pubmed/23864834
http://dx.doi.org/10.1155/2013/801901
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