Effects of External Potassium (K) Supply on Drought Tolerances of Two Contrasting Winter Wheat Cultivars

BACKGROUND: Drought is a common stress limiting crops growth and productivities worldwide. Water deficit may increase cellular membrane permeability, resulting in K outflow. Internal K starvation may disorder plant metabolism and limit plant growth. However, it is seldom reported about the effects of external K on drought tolerance of contrasting wheat cultivars. METHODOLOGY/PRINCIPAL FINDINGS: A hydroponics experiment was carried out in a non-controlled greenhouse. Seedlings of drought-tolerant SN16 and intolerant JM22 were simultaneously treated by five levels of K(2)CO(3) (0, 2.5, 5, 7.5, 10 mM) and two levels of PEG6000 (0, 20%) for 7 days. External K(2)CO(3) significantly increased shoot K(+) content, water potential, chlorophyll content as well as gas exchange, but decreased electrolyte leakage (EL) and MDA content in both cultivars under PEG6000 stress. Antioxidant enzymes activities were up-regulated by PEG6000 while external K(2)CO(3) reduced those changes. Molecular basis was explained by measuring the expression levels of antioxidant enzymes related genes. Shoot and root biomass were also increased by K(2)CO(3) supply under drought stress. Although adequate K(2)CO(3) application enhanced plant growth for both cultivars under drought stress, SN16 was better than JM22 due to its high drought tolerance. CONCLUSIONS/SIGNIFICANCE: Adequate external K may effectively protect winter wheat from drought injuries. We conclude that drought-tolerant wheat combined with adequate external K supply may be a promising strategy for better growth in arid and semi-arid regions.