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Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells
The migratory capability of cancer cells is one of the most important hallmarks reflecting metastatic potential. Ouabain, an endogenous cardiac glycoside produced by the adrenal gland, has been previously reported to have anti-tumor activities; however, its role in the regulation of cancer cell migr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707866/ https://www.ncbi.nlm.nih.gov/pubmed/23874694 http://dx.doi.org/10.1371/journal.pone.0068623 |
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author | Pongrakhananon, Varisa Chunhacha, Preedakorn Chanvorachote, Pithi |
author_facet | Pongrakhananon, Varisa Chunhacha, Preedakorn Chanvorachote, Pithi |
author_sort | Pongrakhananon, Varisa |
collection | PubMed |
description | The migratory capability of cancer cells is one of the most important hallmarks reflecting metastatic potential. Ouabain, an endogenous cardiac glycoside produced by the adrenal gland, has been previously reported to have anti-tumor activities; however, its role in the regulation of cancer cell migration remains unknown. The present study has revealed that treatment with ouabain at physiological concentrations is able to inhibit the migratory activities of human lung cancer H292 cells. The negative effects of ouabain were found to be mediated through the suppression of migration regulatory proteins, such as focal adhesion kinase (FAK), ATP-dependent tyrosine kinase (Akt), and cell division cycle 42 (Cdc42). We found that the observed actions of ouabain were mediated via a reactive oxygen species (ROS)-dependent mechanism because the addition of ROS scavengers (N-acetylcysteine and glutathione) could reverse the effect of ouabain on cell migration. Furthermore, ouabain was shown to inhibit the spheroidal tumor growth and decrease the cancer cell adhesion to endothelial cells. However, the compound had no significant effect on anoikis of the cells. Together, these findings shed light on the understanding of cancer cell biology by exploring the novel function of this endogenous human substance. |
format | Online Article Text |
id | pubmed-3707866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37078662013-07-19 Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells Pongrakhananon, Varisa Chunhacha, Preedakorn Chanvorachote, Pithi PLoS One Research Article The migratory capability of cancer cells is one of the most important hallmarks reflecting metastatic potential. Ouabain, an endogenous cardiac glycoside produced by the adrenal gland, has been previously reported to have anti-tumor activities; however, its role in the regulation of cancer cell migration remains unknown. The present study has revealed that treatment with ouabain at physiological concentrations is able to inhibit the migratory activities of human lung cancer H292 cells. The negative effects of ouabain were found to be mediated through the suppression of migration regulatory proteins, such as focal adhesion kinase (FAK), ATP-dependent tyrosine kinase (Akt), and cell division cycle 42 (Cdc42). We found that the observed actions of ouabain were mediated via a reactive oxygen species (ROS)-dependent mechanism because the addition of ROS scavengers (N-acetylcysteine and glutathione) could reverse the effect of ouabain on cell migration. Furthermore, ouabain was shown to inhibit the spheroidal tumor growth and decrease the cancer cell adhesion to endothelial cells. However, the compound had no significant effect on anoikis of the cells. Together, these findings shed light on the understanding of cancer cell biology by exploring the novel function of this endogenous human substance. Public Library of Science 2013-07-10 /pmc/articles/PMC3707866/ /pubmed/23874694 http://dx.doi.org/10.1371/journal.pone.0068623 Text en © 2013 Pongrakhananon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pongrakhananon, Varisa Chunhacha, Preedakorn Chanvorachote, Pithi Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells |
title | Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells |
title_full | Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells |
title_fullStr | Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells |
title_full_unstemmed | Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells |
title_short | Ouabain Suppresses the Migratory Behavior of Lung Cancer Cells |
title_sort | ouabain suppresses the migratory behavior of lung cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707866/ https://www.ncbi.nlm.nih.gov/pubmed/23874694 http://dx.doi.org/10.1371/journal.pone.0068623 |
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