Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation

A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity i...

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Detalles Bibliográficos
Autores principales: Bogdanov, Evgeny, Dominova, Irina, Shusharina, Natalia, Botman, Stepan, Kasymov, Vitaliy, Patrushev, Maksim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707904/
https://www.ncbi.nlm.nih.gov/pubmed/23874571
http://dx.doi.org/10.1371/journal.pone.0068280
Descripción
Sumario:A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon dioxide nanofilm deposited on the inner surface of PCR tube.

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