Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation

A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity i...

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Detalles Bibliográficos
Autores principales: Bogdanov, Evgeny, Dominova, Irina, Shusharina, Natalia, Botman, Stepan, Kasymov, Vitaliy, Patrushev, Maksim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707904/
https://www.ncbi.nlm.nih.gov/pubmed/23874571
http://dx.doi.org/10.1371/journal.pone.0068280
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author Bogdanov, Evgeny
Dominova, Irina
Shusharina, Natalia
Botman, Stepan
Kasymov, Vitaliy
Patrushev, Maksim
author_facet Bogdanov, Evgeny
Dominova, Irina
Shusharina, Natalia
Botman, Stepan
Kasymov, Vitaliy
Patrushev, Maksim
author_sort Bogdanov, Evgeny
collection PubMed
description A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon dioxide nanofilm deposited on the inner surface of PCR tube.
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spelling pubmed-37079042013-07-19 Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation Bogdanov, Evgeny Dominova, Irina Shusharina, Natalia Botman, Stepan Kasymov, Vitaliy Patrushev, Maksim PLoS One Research Article A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon dioxide nanofilm deposited on the inner surface of PCR tube. Public Library of Science 2013-07-10 /pmc/articles/PMC3707904/ /pubmed/23874571 http://dx.doi.org/10.1371/journal.pone.0068280 Text en © 2013 Bogdanov et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bogdanov, Evgeny
Dominova, Irina
Shusharina, Natalia
Botman, Stepan
Kasymov, Vitaliy
Patrushev, Maksim
Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation
title Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation
title_full Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation
title_fullStr Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation
title_full_unstemmed Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation
title_short Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation
title_sort silicon dioxide thin film mediated single cell nucleic acid isolation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707904/
https://www.ncbi.nlm.nih.gov/pubmed/23874571
http://dx.doi.org/10.1371/journal.pone.0068280
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