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Use of a white light supercontinuum laser for confocal interference-reflection microscopy
Shortly after its development, the white light supercontinuum laser was applied to confocal scanning microscopy as a more versatile substitute for the multiple monochromatic lasers normally used for the excitation of fluorescence. This light source is now available coupled to commercial confocal flu...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708109/ https://www.ncbi.nlm.nih.gov/pubmed/22432542 http://dx.doi.org/10.1111/j.1365-2818.2012.03603.x |
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author | Chiu, L-D Su, L Reichelt, S Amos, WB |
author_facet | Chiu, L-D Su, L Reichelt, S Amos, WB |
author_sort | Chiu, L-D |
collection | PubMed |
description | Shortly after its development, the white light supercontinuum laser was applied to confocal scanning microscopy as a more versatile substitute for the multiple monochromatic lasers normally used for the excitation of fluorescence. This light source is now available coupled to commercial confocal fluorescence microscopes. We have evaluated a supercontinuum laser as a source for a different purpose: confocal interferometric imaging of living cells and artificial models by interference reflection. We used light in the range 460–700 nm where this source provides a reasonably flat spectrum, and obtained images free from fringe artefacts caused by the longer coherence length of conventional lasers. We have also obtained images of cytoskeletal detail that is difficult to see with a monochromatic laser. |
format | Online Article Text |
id | pubmed-3708109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37081092013-07-12 Use of a white light supercontinuum laser for confocal interference-reflection microscopy Chiu, L-D Su, L Reichelt, S Amos, WB J Microsc Original Articles Shortly after its development, the white light supercontinuum laser was applied to confocal scanning microscopy as a more versatile substitute for the multiple monochromatic lasers normally used for the excitation of fluorescence. This light source is now available coupled to commercial confocal fluorescence microscopes. We have evaluated a supercontinuum laser as a source for a different purpose: confocal interferometric imaging of living cells and artificial models by interference reflection. We used light in the range 460–700 nm where this source provides a reasonably flat spectrum, and obtained images free from fringe artefacts caused by the longer coherence length of conventional lasers. We have also obtained images of cytoskeletal detail that is difficult to see with a monochromatic laser. Blackwell Publishing Ltd 2012-05 /pmc/articles/PMC3708109/ /pubmed/22432542 http://dx.doi.org/10.1111/j.1365-2818.2012.03603.x Text en © 2012 The Authors Journal of Microscopy © 2012 Royal Microscopical Society http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Original Articles Chiu, L-D Su, L Reichelt, S Amos, WB Use of a white light supercontinuum laser for confocal interference-reflection microscopy |
title | Use of a white light supercontinuum laser for confocal interference-reflection microscopy |
title_full | Use of a white light supercontinuum laser for confocal interference-reflection microscopy |
title_fullStr | Use of a white light supercontinuum laser for confocal interference-reflection microscopy |
title_full_unstemmed | Use of a white light supercontinuum laser for confocal interference-reflection microscopy |
title_short | Use of a white light supercontinuum laser for confocal interference-reflection microscopy |
title_sort | use of a white light supercontinuum laser for confocal interference-reflection microscopy |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708109/ https://www.ncbi.nlm.nih.gov/pubmed/22432542 http://dx.doi.org/10.1111/j.1365-2818.2012.03603.x |
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