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Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit

The central nonsense-mediated mRNA decay (NMD) regulator, Upf1, selectively targets nonsense-containing mRNAs for rapid degradation. In yeast, Upf1 preferentially associates with mRNAs that are NMD substrates, but the mechanism of its selective retention on these mRNAs has yet to be elucidated. Prev...

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Detalles Bibliográficos
Autores principales: Min, Ei Ei, Roy, Bijoyita, Amrani, Nadia, He, Feng, Jacobson, Allan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708530/
https://www.ncbi.nlm.nih.gov/pubmed/23801788
http://dx.doi.org/10.1261/rna.039396.113
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author Min, Ei Ei
Roy, Bijoyita
Amrani, Nadia
He, Feng
Jacobson, Allan
author_facet Min, Ei Ei
Roy, Bijoyita
Amrani, Nadia
He, Feng
Jacobson, Allan
author_sort Min, Ei Ei
collection PubMed
description The central nonsense-mediated mRNA decay (NMD) regulator, Upf1, selectively targets nonsense-containing mRNAs for rapid degradation. In yeast, Upf1 preferentially associates with mRNAs that are NMD substrates, but the mechanism of its selective retention on these mRNAs has yet to be elucidated. Previously, we demonstrated that Upf1 associates with 40S ribosomal subunits. Here, we define more precisely the nature of this association using conventional and affinity-based purification of ribosomal subunits, and a two-hybrid screen to identify Upf1-interacting ribosomal proteins. Upf1 coimmunoprecipitates specifically with epitope-tagged 40S ribosomal subunits, and Upf1 association with high-salt washed or puromycin-released 40S subunits was found to occur without simultaneous eRF1, eRF3, Upf2, or Upf3 association. Two-hybrid analyses and in vitro binding assays identified a specific interaction between Upf1 and Rps26. Using mutations in domains of UPF1 known to be crucial for its function, we found that Upf1:40S association is modulated by ATP, and Upf1:Rps26 interaction is dependent on the N-terminal Upf1 CH domain. The specific association of Upf1 with the 40S subunit is consistent with the notion that this RNA helicase not only triggers rapid decay of nonsense-containing mRNAs, but may also have an important role in dissociation of the premature termination complex.
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spelling pubmed-37085302014-08-01 Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit Min, Ei Ei Roy, Bijoyita Amrani, Nadia He, Feng Jacobson, Allan RNA Articles The central nonsense-mediated mRNA decay (NMD) regulator, Upf1, selectively targets nonsense-containing mRNAs for rapid degradation. In yeast, Upf1 preferentially associates with mRNAs that are NMD substrates, but the mechanism of its selective retention on these mRNAs has yet to be elucidated. Previously, we demonstrated that Upf1 associates with 40S ribosomal subunits. Here, we define more precisely the nature of this association using conventional and affinity-based purification of ribosomal subunits, and a two-hybrid screen to identify Upf1-interacting ribosomal proteins. Upf1 coimmunoprecipitates specifically with epitope-tagged 40S ribosomal subunits, and Upf1 association with high-salt washed or puromycin-released 40S subunits was found to occur without simultaneous eRF1, eRF3, Upf2, or Upf3 association. Two-hybrid analyses and in vitro binding assays identified a specific interaction between Upf1 and Rps26. Using mutations in domains of UPF1 known to be crucial for its function, we found that Upf1:40S association is modulated by ATP, and Upf1:Rps26 interaction is dependent on the N-terminal Upf1 CH domain. The specific association of Upf1 with the 40S subunit is consistent with the notion that this RNA helicase not only triggers rapid decay of nonsense-containing mRNAs, but may also have an important role in dissociation of the premature termination complex. Cold Spring Harbor Laboratory Press 2013-08 /pmc/articles/PMC3708530/ /pubmed/23801788 http://dx.doi.org/10.1261/rna.039396.113 Text en © 2013; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.
spellingShingle Articles
Min, Ei Ei
Roy, Bijoyita
Amrani, Nadia
He, Feng
Jacobson, Allan
Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit
title Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit
title_full Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit
title_fullStr Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit
title_full_unstemmed Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit
title_short Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit
title_sort yeast upf1 ch domain interacts with rps26 of the 40s ribosomal subunit
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708530/
https://www.ncbi.nlm.nih.gov/pubmed/23801788
http://dx.doi.org/10.1261/rna.039396.113
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