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MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation
Mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Investigation of the regulation of clock gene expression has mainly focused on transcriptional and posttranslational modifications, and little is known abou...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708730/ https://www.ncbi.nlm.nih.gov/pubmed/23699394 http://dx.doi.org/10.1091/mbc.E12-12-0849 |
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author | Lee, Kyung-Ha Kim, Sung-Hoon Lee, Hwa-Rim Kim, Wanil Kim, Do-Yeon Shin, Jae-Cheon Yoo, Seung-Hee Kim, Kyong-Tai |
author_facet | Lee, Kyung-Ha Kim, Sung-Hoon Lee, Hwa-Rim Kim, Wanil Kim, Do-Yeon Shin, Jae-Cheon Yoo, Seung-Hee Kim, Kyong-Tai |
author_sort | Lee, Kyung-Ha |
collection | PubMed |
description | Mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Investigation of the regulation of clock gene expression has mainly focused on transcriptional and posttranslational modifications, and little is known about the posttranscriptional regulation of these genes. In the present study, we investigate the role of microRNAs (miRNAs) in the posttranscriptional regulation of the 3′-untranslated region (UTR) of the mouse Cryptochrome 1 (mCry1) gene. Knockdown of Drosha, Dicer, or Argonaute2 increased mCry1-3′UTR reporter activity. The presence of the miRNA recognition element of mCry1 that is important for miR-185 binding decreased mCRY1 protein, but not mRNA, level. Cytoplasmic miR-185 levels were nearly antiphase to mCRY1 protein levels. Furthermore, miR-185 knockdown elevated the amplitude of mCRY1 protein oscillation. Our results suggest that miR-185 plays a role in the fine-tuned regulation of mCRY1 protein expression by controlling the rhythmicity of mCry1 mRNA translation. |
format | Online Article Text |
id | pubmed-3708730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-37087302013-09-30 MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation Lee, Kyung-Ha Kim, Sung-Hoon Lee, Hwa-Rim Kim, Wanil Kim, Do-Yeon Shin, Jae-Cheon Yoo, Seung-Hee Kim, Kyong-Tai Mol Biol Cell Articles Mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Investigation of the regulation of clock gene expression has mainly focused on transcriptional and posttranslational modifications, and little is known about the posttranscriptional regulation of these genes. In the present study, we investigate the role of microRNAs (miRNAs) in the posttranscriptional regulation of the 3′-untranslated region (UTR) of the mouse Cryptochrome 1 (mCry1) gene. Knockdown of Drosha, Dicer, or Argonaute2 increased mCry1-3′UTR reporter activity. The presence of the miRNA recognition element of mCry1 that is important for miR-185 binding decreased mCRY1 protein, but not mRNA, level. Cytoplasmic miR-185 levels were nearly antiphase to mCRY1 protein levels. Furthermore, miR-185 knockdown elevated the amplitude of mCRY1 protein oscillation. Our results suggest that miR-185 plays a role in the fine-tuned regulation of mCRY1 protein expression by controlling the rhythmicity of mCry1 mRNA translation. The American Society for Cell Biology 2013-07-15 /pmc/articles/PMC3708730/ /pubmed/23699394 http://dx.doi.org/10.1091/mbc.E12-12-0849 Text en © 2013 Lee et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Lee, Kyung-Ha Kim, Sung-Hoon Lee, Hwa-Rim Kim, Wanil Kim, Do-Yeon Shin, Jae-Cheon Yoo, Seung-Hee Kim, Kyong-Tai MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation |
title | MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation |
title_full | MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation |
title_fullStr | MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation |
title_full_unstemmed | MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation |
title_short | MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation |
title_sort | microrna-185 oscillation controls circadian amplitude of mouse cryptochrome 1 via translational regulation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708730/ https://www.ncbi.nlm.nih.gov/pubmed/23699394 http://dx.doi.org/10.1091/mbc.E12-12-0849 |
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