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MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation

Mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Investigation of the regulation of clock gene expression has mainly focused on transcriptional and posttranslational modifications, and little is known abou...

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Autores principales: Lee, Kyung-Ha, Kim, Sung-Hoon, Lee, Hwa-Rim, Kim, Wanil, Kim, Do-Yeon, Shin, Jae-Cheon, Yoo, Seung-Hee, Kim, Kyong-Tai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708730/
https://www.ncbi.nlm.nih.gov/pubmed/23699394
http://dx.doi.org/10.1091/mbc.E12-12-0849
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author Lee, Kyung-Ha
Kim, Sung-Hoon
Lee, Hwa-Rim
Kim, Wanil
Kim, Do-Yeon
Shin, Jae-Cheon
Yoo, Seung-Hee
Kim, Kyong-Tai
author_facet Lee, Kyung-Ha
Kim, Sung-Hoon
Lee, Hwa-Rim
Kim, Wanil
Kim, Do-Yeon
Shin, Jae-Cheon
Yoo, Seung-Hee
Kim, Kyong-Tai
author_sort Lee, Kyung-Ha
collection PubMed
description Mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Investigation of the regulation of clock gene expression has mainly focused on transcriptional and posttranslational modifications, and little is known about the posttranscriptional regulation of these genes. In the present study, we investigate the role of microRNAs (miRNAs) in the posttranscriptional regulation of the 3′-untranslated region (UTR) of the mouse Cryptochrome 1 (mCry1) gene. Knockdown of Drosha, Dicer, or Argonaute2 increased mCry1-3′UTR reporter activity. The presence of the miRNA recognition element of mCry1 that is important for miR-185 binding decreased mCRY1 protein, but not mRNA, level. Cytoplasmic miR-185 levels were nearly antiphase to mCRY1 protein levels. Furthermore, miR-185 knockdown elevated the amplitude of mCRY1 protein oscillation. Our results suggest that miR-185 plays a role in the fine-tuned regulation of mCRY1 protein expression by controlling the rhythmicity of mCry1 mRNA translation.
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spelling pubmed-37087302013-09-30 MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation Lee, Kyung-Ha Kim, Sung-Hoon Lee, Hwa-Rim Kim, Wanil Kim, Do-Yeon Shin, Jae-Cheon Yoo, Seung-Hee Kim, Kyong-Tai Mol Biol Cell Articles Mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Investigation of the regulation of clock gene expression has mainly focused on transcriptional and posttranslational modifications, and little is known about the posttranscriptional regulation of these genes. In the present study, we investigate the role of microRNAs (miRNAs) in the posttranscriptional regulation of the 3′-untranslated region (UTR) of the mouse Cryptochrome 1 (mCry1) gene. Knockdown of Drosha, Dicer, or Argonaute2 increased mCry1-3′UTR reporter activity. The presence of the miRNA recognition element of mCry1 that is important for miR-185 binding decreased mCRY1 protein, but not mRNA, level. Cytoplasmic miR-185 levels were nearly antiphase to mCRY1 protein levels. Furthermore, miR-185 knockdown elevated the amplitude of mCRY1 protein oscillation. Our results suggest that miR-185 plays a role in the fine-tuned regulation of mCRY1 protein expression by controlling the rhythmicity of mCry1 mRNA translation. The American Society for Cell Biology 2013-07-15 /pmc/articles/PMC3708730/ /pubmed/23699394 http://dx.doi.org/10.1091/mbc.E12-12-0849 Text en © 2013 Lee et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Lee, Kyung-Ha
Kim, Sung-Hoon
Lee, Hwa-Rim
Kim, Wanil
Kim, Do-Yeon
Shin, Jae-Cheon
Yoo, Seung-Hee
Kim, Kyong-Tai
MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation
title MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation
title_full MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation
title_fullStr MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation
title_full_unstemmed MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation
title_short MicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation
title_sort microrna-185 oscillation controls circadian amplitude of mouse cryptochrome 1 via translational regulation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708730/
https://www.ncbi.nlm.nih.gov/pubmed/23699394
http://dx.doi.org/10.1091/mbc.E12-12-0849
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