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Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1

BACKGROUND: Nine gene clusters dedicated to nonribosomal synthesis of secondary metabolites with possible antimicrobial action, including polymyxin and fusaricidin, were detected within the whole genome sequence of the plant growth-promoting rhizobacterium (PGPR) Paenibacillus polymyxa M-1. To surve...

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Autores principales: Niu, Ben, Vater, Joachim, Rueckert, Christian, Blom, Jochen, Lehmann, Maik, Ru, Jin-Jiang, Chen, Xiao-Hua, Wang, Qi, Borriss, Rainer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708778/
https://www.ncbi.nlm.nih.gov/pubmed/23773687
http://dx.doi.org/10.1186/1471-2180-13-137
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author Niu, Ben
Vater, Joachim
Rueckert, Christian
Blom, Jochen
Lehmann, Maik
Ru, Jin-Jiang
Chen, Xiao-Hua
Wang, Qi
Borriss, Rainer
author_facet Niu, Ben
Vater, Joachim
Rueckert, Christian
Blom, Jochen
Lehmann, Maik
Ru, Jin-Jiang
Chen, Xiao-Hua
Wang, Qi
Borriss, Rainer
author_sort Niu, Ben
collection PubMed
description BACKGROUND: Nine gene clusters dedicated to nonribosomal synthesis of secondary metabolites with possible antimicrobial action, including polymyxin and fusaricidin, were detected within the whole genome sequence of the plant growth-promoting rhizobacterium (PGPR) Paenibacillus polymyxa M-1. To survey the antimicrobial compounds expressed by M-1 we analyzed the active principle suppressing phytopathogenic Erwinia spp. RESULTS: P. polymyxa M-1 suppressed the growth of phytopathogenic Erwinia amylovora Ea 273, and E. carotovora, the causative agents of fire blight and soft rot, respectively. By MALDI-TOF mass spectrometry and reversed-phase high-performance liquid chromatography (RP-HPLC), two antibacterial compounds bearing molecular masses of 1190.9 Da and 1176.9 Da were detected as being the two components of polymyxin P, polymyxin P(1) and P(2), respectively. The active principle acting against the two Erwinia strains was isolated from TLC plates and identified by postsource decay (PSD)-MALDI-TOF mass spectrometry as polymyxin P(1) and polymyxin P(2). These findings were corroborated by domain structure analysis of the polymyxin (pmx) gene cluster detected in the M-1 chromosome which revealed that corresponding to the chemical structure of polymyxin P, the gene cluster is encoding D-Phe in position 6 and L-Thr in position 7. CONCLUSIONS: Identical morphological changes in the cell wall of the bacterial phytopathogens treated with either crude polymyxin P or culture supernatant of M-1 corroborated that polymyxin P is the main component of the biocontrol effect exerted by strain M-1 against phytopathogenic Erwinia spp.
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spelling pubmed-37087782013-07-12 Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1 Niu, Ben Vater, Joachim Rueckert, Christian Blom, Jochen Lehmann, Maik Ru, Jin-Jiang Chen, Xiao-Hua Wang, Qi Borriss, Rainer BMC Microbiol Research Article BACKGROUND: Nine gene clusters dedicated to nonribosomal synthesis of secondary metabolites with possible antimicrobial action, including polymyxin and fusaricidin, were detected within the whole genome sequence of the plant growth-promoting rhizobacterium (PGPR) Paenibacillus polymyxa M-1. To survey the antimicrobial compounds expressed by M-1 we analyzed the active principle suppressing phytopathogenic Erwinia spp. RESULTS: P. polymyxa M-1 suppressed the growth of phytopathogenic Erwinia amylovora Ea 273, and E. carotovora, the causative agents of fire blight and soft rot, respectively. By MALDI-TOF mass spectrometry and reversed-phase high-performance liquid chromatography (RP-HPLC), two antibacterial compounds bearing molecular masses of 1190.9 Da and 1176.9 Da were detected as being the two components of polymyxin P, polymyxin P(1) and P(2), respectively. The active principle acting against the two Erwinia strains was isolated from TLC plates and identified by postsource decay (PSD)-MALDI-TOF mass spectrometry as polymyxin P(1) and polymyxin P(2). These findings were corroborated by domain structure analysis of the polymyxin (pmx) gene cluster detected in the M-1 chromosome which revealed that corresponding to the chemical structure of polymyxin P, the gene cluster is encoding D-Phe in position 6 and L-Thr in position 7. CONCLUSIONS: Identical morphological changes in the cell wall of the bacterial phytopathogens treated with either crude polymyxin P or culture supernatant of M-1 corroborated that polymyxin P is the main component of the biocontrol effect exerted by strain M-1 against phytopathogenic Erwinia spp. BioMed Central 2013-06-18 /pmc/articles/PMC3708778/ /pubmed/23773687 http://dx.doi.org/10.1186/1471-2180-13-137 Text en Copyright © 2013 Niu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Niu, Ben
Vater, Joachim
Rueckert, Christian
Blom, Jochen
Lehmann, Maik
Ru, Jin-Jiang
Chen, Xiao-Hua
Wang, Qi
Borriss, Rainer
Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1
title Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1
title_full Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1
title_fullStr Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1
title_full_unstemmed Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1
title_short Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1
title_sort polymyxin p is the active principle in suppressing phytopathogenic erwinia spp. by the biocontrol rhizobacterium paenibacillus polymyxa m-1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708778/
https://www.ncbi.nlm.nih.gov/pubmed/23773687
http://dx.doi.org/10.1186/1471-2180-13-137
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